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Keivanloo, Saeide, Sudagar, Mohammad, Mazandarani, Mohammad. (1398). Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C. سامانه مدیریت نشریات علمی, 18(4), 1035-1045. doi: 10.22092/ijfs.2019.118495
Saeide Keivanloo; Mohammad Sudagar; Mohammad Mazandarani. "Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C". سامانه مدیریت نشریات علمی, 18, 4, 1398, 1035-1045. doi: 10.22092/ijfs.2019.118495
Keivanloo, Saeide, Sudagar, Mohammad, Mazandarani, Mohammad. (1398). 'Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C', سامانه مدیریت نشریات علمی, 18(4), pp. 1035-1045. doi: 10.22092/ijfs.2019.118495
Keivanloo, Saeide, Sudagar, Mohammad, Mazandarani, Mohammad. Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C. سامانه مدیریت نشریات علمی, 1398; 18(4): 1035-1045. doi: 10.22092/ijfs.2019.118495

Evaluating the suitability of cryopreservation solutions for common carp (Cyprinus carpio) embryos stored at -2 °C

Iranian Journal of Fisheries Sciences
مقاله 157، دوره 18، شماره 4، دی 2019، صفحه 1035-1045    اصل مقاله (240.51 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2019.118495
نویسندگان
Saeide Keivanloo1؛ Mohammad Sudagar* 2؛ Mohammad Mazandarani3
1University of Agricultural Science and Natural Res
2د.گرگان
3Gorgan University of Agricultural Sciences & Natur
چکیده
In the present study, cryopreservation of common carp (Cyprinus carpio) embryos was studied using a stepwise cooling protocol. Embryos at heartbeat stage were selected, placed in 15- mL plastic tubes and stored at -2 °C for 1, 7, 14, and 21 days in 2 different cryopreservation solutions: S1 (methanol + dimethyl sulfoxide + sucrose) and S2 (methanol + propylene glycol + sucrose). Embryo viability was assessed by survival rate, counting live larvae and number of failed eggs under a stereomicroscope.  The results showed that storage of common carp embryos was possible for up to 7 days at -2 °C, while the survival did not occur when storage period were 14 and 21 days. The combination of methanol with propylene glycol (S2) gave higher survival rate after 1 and 7 days stored at -2 °C. Further studies are needed in order to extend the storage time and to improve the survival rate for this species.
کلیدواژه‌ها
Cryopreservation؛ common carp؛ Cryoprotectant
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