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اثر پیش تیمار بذر بر افزایش تحمل گیاهچه آفتابگردان در خاک‌های آلوده به سرب در شرایط گلخانه | ||
| علوم و فناوری بذر ایران | ||
| مقاله 11، دوره 7، شماره 2 - شماره پیاپی 14، بهمن 1397، صفحه 153-169 اصل مقاله (944.64 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.109514.1063 | ||
| نویسندگان | ||
| نجمه افتخار1؛ سینا فلاح* 2؛ علی عباسی سورکی3؛ حبیب خداوردی لو4 | ||
| 1دانشجو/ دانشگاه شهرکرد | ||
| 2عضو هیأت علمی دانشگاه شهرکرد | ||
| 3عضو هیأت علمی/ دانشگاه شهرکرد | ||
| 4عضو هیأت علمی/ دانشگاه ارومیه | ||
| چکیده | ||
| در خاک‌های آلوده استقرار ضعیف گیاهچه و در نتیجه محدویت زیست توده گیاه، کارآیی فناوری گیاه‌پالایی را کاهش میدهد. در این پژوهش، اثر سطوح مختلف پیشتیمار بذر ( سالیسیلیک اسید، نیترات پتاسیم و شاهد) و 3 غلظت سرب شامل (صفر، 200، 400 و 600 میلی‌گرم سرب بر کیلوگرم خاک) بر رشد گیاهچهای آفتابگردان بررسی گردید. آزمایش به صورت فاکتوریل در قالب طرح کاملا تصادفی با سه تکرار در گلخانه تحقیقاتی دانشگاه شهرکرد در سال 1393 اجرا شد. بطور کلی صفات مورد مطالعه با افزایش غلظت سرب به‌طور معنی‌داری کاهش یافت. در سمی‌ترین غلظت سرب تیمارهای نیترات پتاسیم و سالیسیلیک اسید مانع از کاهش 42% وزن خشک ریشه نسبت به تیمار شاهد شدند. تیمار نیترات پتاسیم از کاهش وزن خشک برگ و کلرفیل a (به ترتیب 24% و 60%) تیمار و سالیسیلیک اسید از کاهش کلرفیلb (55%) نسبت به بذور پرایم نشده جلوگیری کرد. وزن خشک ساقه با تیمارهای پرایمینگ بذر تغییر معنی‌داری نشان نداد. به موازات افزایش غلظت سرب میزان کاراتنوئید، پرولین و نشت یونی گیاهچه افزایش یافت. این در حالی است تیمار سالیسیلیک اسید موجب کاهش 21% نشت الکترولیتی شد. تیمار نیترات پتاسیم مانع از افزایش 24% کاروتنوئید گردید ولی میزان پرولین از 104 به 111 میکرومول بر گرم افزایش داد. بطور کلی نتیجهگیری میشود که کاربرد سالییلیک اسید و نیترات پتاسیم با کاهش میزان کاروتنوئید و نشت الکترولیتی و افزایش محتوای پرولین بردباری گیاهچه آفتابرگردان را به سمیت سرب افزایش میدهد. | ||
| کلیدواژه‌ها | ||
| پرولین؛ سرب؛ کلروفیل؛ گیاه پالایی؛ نشت الکترولیتی | ||
| عنوان مقاله [English] | ||
| Effect of Seed Pretreatments on Sunflower Seedling Tolerance in Soils Contaminated with Lead under Greenhouse Conditions | ||
| نویسندگان [English] | ||
| S. Fallah2؛ | ||
| 2Faculty Member, Univ. of Shahrekord | ||
| چکیده [English] | ||
| Poor seedling establishment and total plant biomass reduction in contaminated soils, decrease the efficiency of phytoremediation techniques. In this study, the effects of different types of seed pretreatments (such as salicylic acid, and potassium nitrate) and three concentrations of lead (0, 200, 400 and 600 mg lead/kg soil) on sunflower seedling growth under greenhouse conditions were evaluated. These factorial experiment was conducted in completely randomized design with three replications in research greenhouse of Shahrekord University, in 2015. In general, the studied traits significantly decreased with increasing concentrations of lead. In most toxic concentrations of lead, Potassium nitrate and salicylic acid prevented the decreasing of dry weight of root compared to control up to 42%. Potassium nitrate prevented the decreasing of dry weight of leaf and chlorophyll a (with 24% and 60%, respectively), Salicylic acid prevented the decreasing of chlorophyll b (55%) compared to control. Shoot dry weight didn’t affect by seed priming treatments. Increasing in concentrations of lead elevated carotenoids, proline content and electrolyte leakage. Salicylic acid treatment reduced of electrolyte leakage (by 21%). Potassium nitrate prevents the increasing of carotenoids (by 24%), however increased proline content from 104 to 111 µm/g. Generally it could be concluded that salicylic acid and potassium nitrate increase seedling tolerance to lead toxicity through reducing carotenoids content, electrolyte leakage and increasing proline content. This technique can be used to increase the efficiency of phytoremediation of soils contaminated with lead. | ||
| کلیدواژه‌ها [English] | ||
| chlorophyll, electrolyte leakage, Lead, phytoremediation, Proline | ||
| مراجع | ||
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