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Gheisary, Behnam, Hosseini, Bahman, Hassanpour, Hamid, Rahimi, Amir. (1397). Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots. سامانه مدیریت نشریات علمی, 7(2), 135-144. doi: 10.22092/jmpb.2018.118141
Behnam Gheisary; Bahman Hosseini; Hamid Hassanpour; Amir Rahimi. "Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots". سامانه مدیریت نشریات علمی, 7, 2, 1397, 135-144. doi: 10.22092/jmpb.2018.118141
Gheisary, Behnam, Hosseini, Bahman, Hassanpour, Hamid, Rahimi, Amir. (1397). 'Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots', سامانه مدیریت نشریات علمی, 7(2), pp. 135-144. doi: 10.22092/jmpb.2018.118141
Gheisary, Behnam, Hosseini, Bahman, Hassanpour, Hamid, Rahimi, Amir. Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots. سامانه مدیریت نشریات علمی, 1397; 7(2): 135-144. doi: 10.22092/jmpb.2018.118141

Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots

Journal of Medicinal plants and By-product
مقاله 2، دوره 7، شماره 2، آذر 2018، صفحه 135-144    اصل مقاله (314.89 K)
شناسه دیجیتال (DOI): 10.22092/jmpb.2018.118141
نویسندگان
Behnam Gheisary1؛ Bahman Hosseini* 1؛ Hamid Hassanpour1؛ Amir Rahimi2
1Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
2Department of Agronomy, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده
Lattice henbane (Hyoscyamus reticulatus L.) is an herbaceous, biennial plant belonging to Solanaceae family. H. reticulatus hairy roots were established from two-week-old leaves infected by A7 strain of Agrobacterium rhizogenes on solid Murashige and Skoog (MS) medium. In this study, abiotic elicitors including; Sodium silicate (Na2SiO3) with different concentrations (0, 1, 5 and 7 mM) and silver nitrate (AgNO3) concentrations (0, 0.5, 1 and 2 mM) were added to hairy roots culture media. The results showed that, Na2SiO3 and AgNO3 significantly affected hairy roots fresh weight after 24h. Also, the highest hairy root fresh weight was observed in the control, and with broadening elicitor concentrations, fresh weight was decreased in both treated hairy roots with AgNO3 and Na2SiO3 but the effect of exposure duration was not significant. Biochemical analysis showed that total antioxidant activity (TAA), total phenol (TP), catalase (CAT), ascorbate peroxidase (APX) and Guaiacolperoxidase (GPX) activities were enhanced in elicitated hairy roots compared to non elicitated hairy roots. The highest CAT, APX and GPX activities were observed in hairy roots treated with 7mM Na2SiO3 and 2mM AgNO3. Our results suggest that, Na2SiO3 and AgNO3 can stimulate the antioxidant defense systems and protect the plants from subsequent stresses.
کلیدواژه‌ها
Agrobacterium rhizogenes؛ Hyoscyamus؛ Abiotic stresses؛ Total antioxidant
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