اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها3,016
تعداد مقالات33,722
تعداد مشاهده مقاله44,955,621
تعداد دریافت فایل اصل مقاله45,749,209
Gheisary, Behnam, Hosseini, Bahman, Hassanpour, Hamid, Rahimi, Amir. (1397). Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots. سامانه مدیریت نشریات علمی, 7(2), 135-144. doi: 10.22092/jmpb.2018.118141
Behnam Gheisary; Bahman Hosseini; Hamid Hassanpour; Amir Rahimi. "Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots". سامانه مدیریت نشریات علمی, 7, 2, 1397, 135-144. doi: 10.22092/jmpb.2018.118141
Gheisary, Behnam, Hosseini, Bahman, Hassanpour, Hamid, Rahimi, Amir. (1397). 'Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots', سامانه مدیریت نشریات علمی, 7(2), pp. 135-144. doi: 10.22092/jmpb.2018.118141
Gheisary, Behnam, Hosseini, Bahman, Hassanpour, Hamid, Rahimi, Amir. Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots. سامانه مدیریت نشریات علمی, 1397; 7(2): 135-144. doi: 10.22092/jmpb.2018.118141

Effects of Silicon and AgNO3 Elicitors on Biochemical Traits and Antioxidant Enzymes Activity of Henbane (Hyoscyamus reticulatus L.) Hairy Roots

Journal of Medicinal plants and By-products
مقاله 2، دوره 7، شماره 2، آذر 2018، صفحه 135-144    اصل مقاله (314.89 K)
شناسه دیجیتال (DOI): 10.22092/jmpb.2018.118141
نویسندگان
Behnam Gheisary1؛ Bahman Hosseini* 1؛ Hamid Hassanpour1؛ Amir Rahimi2
1Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
2Department of Agronomy, Faculty of Agriculture, Urmia University, Urmia, Iran
چکیده
Lattice henbane (Hyoscyamus reticulatus L.) is an herbaceous, biennial plant belonging to Solanaceae family. H. reticulatus hairy roots were established from two-week-old leaves infected by A7 strain of Agrobacterium rhizogenes on solid Murashige and Skoog (MS) medium. In this study, abiotic elicitors including; Sodium silicate (Na2SiO3) with different concentrations (0, 1, 5 and 7 mM) and silver nitrate (AgNO3) concentrations (0, 0.5, 1 and 2 mM) were added to hairy roots culture media. The results showed that, Na2SiO3 and AgNO3 significantly affected hairy roots fresh weight after 24h. Also, the highest hairy root fresh weight was observed in the control, and with broadening elicitor concentrations, fresh weight was decreased in both treated hairy roots with AgNO3 and Na2SiO3 but the effect of exposure duration was not significant. Biochemical analysis showed that total antioxidant activity (TAA), total phenol (TP), catalase (CAT), ascorbate peroxidase (APX) and Guaiacolperoxidase (GPX) activities were enhanced in elicitated hairy roots compared to non elicitated hairy roots. The highest CAT, APX and GPX activities were observed in hairy roots treated with 7mM Na2SiO3 and 2mM AgNO3. Our results suggest that, Na2SiO3 and AgNO3 can stimulate the antioxidant defense systems and protect the plants from subsequent stresses.
کلیدواژه‌ها
Agrobacterium rhizogenes؛ Hyoscyamus؛ Abiotic stresses؛ Total antioxidant
مراجع
1. Deivis TH. Flora of Turkey. Edinburg, University Press, 1978.
2. Evans WC. Tropan alkaloids of the Solanaceae. In: J.G. Hawkes; R.N. Leister & A.D. Skelding (Ed), the biology and taxonomy of the solanaceae. (pp. 241-254.) Academic Press, London, 1979.
3. Oksman-Caldentey KM, Arroo R. Regulation of tropane alkaloid metabolism in plants and plant cell cultures. In: R. Verpoorte & A.W. Alfermann (Ed), metabolic engineering of plant secondary metabolism. (pp. 253-281.) Kluwer, Dordrecht, 2000.
4. Bulgakov VP. Functions of rol genes in plant secondary metabolism. BiotechnolAdv. 2008;26:318-324.
5. Shkryl YN, Veremeichik GN, Bulgakov VP, Tchernoded GK, Mischenko SA, Fedoreyev NP, Zhuravlev YN. Individual and combined effects of the rolA, B, and C genes on anthraquinone production in Rubia cordifolia transformed calli. Biotechnol Bioeng. 2008;100:118-125.
6. Zaheer M, Reddy VD, Giri, CC. Enhanced daidzin production from jasmonic and acetyl salicylic acid elicited hairy root cultures of Psoralea corylifolia L. (Fabaceae). Nat Prod Res. 2016;30:1542-1547.
7. Sauer H, Wartenberg M, Hescheler J. Reactive oxygen species as intracellular messengers during cell growth and differentiation. Cell Physiol Biochem. 2011;11: 173-186.
8. Soleimani T, Keyhanfer M, Piri KH, Hsanloo T. Morphological evaluation of hairy roots induced in Artemisia annua L. and investigating elicitation effects on hairy roots biomass production. Int J Agric Res Rev. 2012;2:1005-1013.
9. Russowski D, Maurmann N, Rech SB, Fett-Neto AG. Improved production of bioactive valepotriates in whole-plant liquid cultures of Valeriana glechomifolia. Ind Crop Prod. 2013;46: 253–257.
10. Liang YC, Sun WC, Zhu YG, Christie P.. Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environ Pollut. 2007;147: 422-428.
11. Sivanesan I, Jeong BR. Silicon promotes adventitious shoot regeneration and enhances salinity tolerance of Ajuga multiflora Bunge by altering activity of antioxidant enzymes. Sci World J. 2014;1-10.
12. Lee SH, Ahsan N, Lee KW, Kim DH, Lee DG, Kwak SS, Kwon SY, Kim TH, Lee BH. Simultaneous over expression of both CuZn superoxide dismutase and ascorbate peroxidase in transgenic tall fescue plants confers increased tolerance to a wide range of abiotic stresses. J Plant Physiol. 2007;164:1626-1638.
13. Bais HP, Sudha G, Ravishankar GA. Influence of putrescine, silver nitrate and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Cichorium intybus and their regenerants. Plant Cell Rep. 2001;20:547-555.
14. Murashige T, Skoog F.A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-497.
15. Bertani G. Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. J Bacteriol. 1952;62:293-300.
16. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vit. 1965;16: 144-158.
17. Brand-Williams W, Cuvellier ME, Berset C. (1995). Use of a free radical to evaluate antioxidant activity. LWT- Food Sci Technol.1995;28:25-30.
18. Kang HM, Saltiveit ME. Chilling tolerance of maize, cucumber and rice seedling (leaves and roots) and differently affected by salicylic acid. Physiol Plant.2002;115:577-576.
19. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 1981;22:867-880.
20. Aebi H. Catalase in vitro.Methods Enzymol. 1984;105: 121-126.
21. Upadhyaya A, Sankhla D, Davis TD, Sankhla N, Smidth BN. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves. J Plant Physiol. 1985;121:453-461.
22. Khan S, Irfan QM, Kamaluddin AT, Abdin MZ. Protocol for isolation of genomic DNA from dry and fresh roots of medicinal plants suitable for RAPD and restriction digestion. Afr J Biotechnol. 2007;26: 175-178.
23. Dragisic Maksimovic J, Bogdanovic J, Maksimovic V, Nikolic M. Silicon modulates the metabolism and utilization of phenolic compounds in cucumber (Cucumis sativus L.) grown at excess manganese. Plant Nutr Soil Sci. 2007;170:739-44.
24. Wang JW, Wu JY. Effective elicitors and process strategies for enhancement of secondary metabolite production in hairy root cultures. Adv Biochem Eng Biotechnol.2013;134:55-89.
25. Zeynali Z, Hosseini B, Rezaei E. Effect of elicitation on antioxidant activity and production of tropane alkaloids in Hyoscyamus reticulatus hairy root cultures. Res J Pharmacogn. 2016;3:43-53.
26. Wu JY, Ng J, Shi M, Wu SJ. Enhanced secondary metabolite (tanshinone) production of Salvia miltiorrhiza hairy roots in a novel root-bacteria co-culture process. Appl Microbiol Biotechnol.2007;77: 543-550.
27. Zhang P, Phansiri S, Puonti KJ. Improvements of cassava shoot organogenesis by the use of silver nitrate in vitro.Plant Cell, Tiss Org Cult. 2001;67: 47-54.
28. Ye M, Song Y, Long J, Wang R, Baerson SR, Pan Z, Zhu-Salzman K, Xie J, Cai K, Luo S, Zeng R. Priming of jasmonate-mediated anti herbivore defense responses in rice by silicon. Proceedings of the National Academy of Sciences of the United States of America 2013;110: 1-9.
29. Zhang FQ, Wang YS, Lou ZP, Dong JD. Effect of heavy metal stress on antioxidative enzymes and lipid peroxidation in leaves and roots of two mangrove plant seedlings (Kandelia candel and Bruguiera gymnorrhiza). Chemosphere. 2007;67:44-50.
30. Song AL, Li P, Li ZJ, Fan FL, Nikolic M, Liang YC. The alleviation of zinc toxicity by silicon is related to zinc transport and antioxidative reactions in rice. Plant & Soil. 2001;344:319-33.
31. Ziv M. Silicon effects on growth acclimatization and stress tolerance of bioreactor cultured Ornithogalum dubium plants. Acta Hort. 2010;865: 29-3.
32. Bulgakov VP, Gorpenchenko TY, Veremeichik GN, Shkryl YN, Tchernoded GK, Bulgakov DV, Aminin DL, Zhuravlev Y.N. The rolB gene suppresses reactive oxygen species in transformed plant cells through the sustained activation of antioxidant defense. Plant Physiol. 2012;158:1371-1381.
33. Nikravesh F, Khavari-Nejad RA, Rahimian H, Fahimi H. Study of antioxidant enzymes activity and isozymes pattern in hairy roots and regenerated plants in Nicotiana tabacum. Acta Physiol Plant. 2012;34:419-427.

آمار
تعداد مشاهده مقاله: 707
تعداد دریافت فایل اصل مقاله: 556


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب