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ایجاد و تعیین ویژگی‌های یک رده سلولی جدید از هموسیت‌های لارو زنبور برگخوار رز Arge ochropus (Hymenoptera; Argidae) | ||
| نامه انجمن حشره شناسی ایران | ||
| مقاله 3، دوره 38، شماره 2 - شماره پیاپی 74، شهریور 1397، صفحه 173-186 اصل مقاله (938.28 K) | ||
| نوع مقاله: مقاله کامل، انگلیسی | ||
| شناسه دیجیتال (DOI): 10.22117/jesi.2018.116228.1155 | ||
| نویسندگان | ||
| جلال جلالی سندی* 1؛ بیتا والی زاده2؛ رویا خسروی3؛ رسول صالحی4 | ||
| 1دانشگاه گیلان | ||
| 2گروه گیاه پزشکی دانشگاه گیلان | ||
| 3گیاه پزشکی دانشگاه گیلان | ||
| 4دانشگاه علوم پزشکی اصفهان | ||
| چکیده | ||
| یک ردهی سلولی جدید از سلولهای خونی لارو زنبور برگخوار رز، Arge ochropusایجاد شد. این رده سلولی براساس نام Arge ochropus University of Guilan، AOU-G نامگذاری شد. این ردهی سلولی از سلولهای خونی لارو زنبور برگخوار رز، به منظور ارزیابی خصوصیات و حساسیت به اکدایزون مورد بررسی قرار گرفت. ویژگی‌های شکل‌شناسی سلولهای آن با استفاده از میکروسکوپ‌های نوری بررسی شد. از نظر ریخت شناسی در کشت اولیه سلولهای خونی، چهار نوع سلول مشهود بود که بهطور عمده شامل پروهموسیت‌ها، پلاسماتوسیت‌ها، گرانولوسیت‌ها و انوسیتوئیدها بود. زمان مضاعف شدن جمعیت این رده سلولی در بیستمین کشت مجدد در دمای 28 درجهی سلسیوس روی محیط کشت Ex-cell 400 و گریس بهترتیب 3 و 5 روز بود. مطالعات بیوشیمیایی روی کشت اولیه ناشی از سلولهای خونی در محیط کشت Ex-cell 400 و گریس، نمایانگر تغییرات قابل ملاحظهای در برخی از ترکیبات نظیر گلوکز، پروتئین، اوره و کلسترول در محیط کشت بود. به غیر از اوره که افزایش معنیداری را در محیط کشت نشان داد، بقیه ترکیبات اندازهگیری شده کاهش چشمگیری پیدا نمودند. ردة سلولی AOU-G را به عنوان یک ردة سلولی جدید معرفی میکنیم که میتواند در مطالعات مربوط به زیست‌شناسی، ایمونولوژی، غدد درون ریز وسمشناسی بسیار مفید باشد. | ||
| کلیدواژه‌ها | ||
| زنبور برگخوار رز؛ باکولوویروس؛ کشت سلول؛ اکدایزون؛ هموسیت | ||
| عنوان مقاله [English] | ||
| Establishment and characterizations of a new cell line from larval hemocytes of rose sawfly Arge ochropus (Hymenoptera; Argidae) | ||
| نویسندگان [English] | ||
| J. Jalali-sendi1؛ B. Valizade2؛ R. Khosravi3؛ R. Salehi4 | ||
| 2Plant Protection, University of Guilan | ||
| 3Plant Protection, University of Guilan | ||
| 4University of Isfahan | ||
| چکیده [English] | ||
| We established and characterized a hymenopteran cell line from Arge ochropus for the first time. The established cell line was named AOU-G, standing for Arge ochropus University of Guilan. This cell line was derived from larval hemocytes of the rose sawfly whose characteristics and susceptibility to 20-hydroxyecdysone have been verified. We also noticed four types of hemocytes such as prohemocytes, plasmatocytes, granulocytes and oenocytes. The population doubling time of 20th passage of this new cell line in EX-cell or Grace medium at 28˚C was 3 and 5 days, respectively. Biochemical studies in rose sawfly hemocytes showed considerable changes in some component: protein, glucose, cholesterol and urea. Except urea, all components were significantly reduced. We suggest that the AOU-G cell line as a productive new cell line that could be very useful in studies concerning bio- insecticides, immunology, endocrinology and toxicology. | ||
| کلیدواژه‌ها [English] | ||
| Arge ochropus, Baculovirus, Cell culture, Ecdysone, Hemocytes | ||
| مراجع | ||
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