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Molecular Detection of Anaplasma Phagocytophilum as a Zoonotic Agent in Owned and Stray Dogs in Tehran, Iran | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 74، شماره 1، خرداد 2019، صفحه 33-38 اصل مقاله (273.17 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.114893.1142 | ||
| نویسندگان | ||
| A. Yousefi* 1؛ M. R. Chaechi Nosrati2؛ A. Golmohammadi2؛ S. Azami3 | ||
| 1Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Parasitology, Faculty of Paraveteriary Medicine, Bu-Ali Sina uiversity, Hamedan | ||
| چکیده | ||
| The genus Anaplasma is an obligated intracellular Rickettsia and among its species, Anaplasma phagocytophilum (A. phagocytophilum) is a zoonotic agent that infects host neutrophils. The aim of this study was molecular detection of A. phagocytophilum infection based on MSP4 gene in owned and stray dogs in Tehran, capital of Iran. One hundred and fifty blood samples were collected from dogs in Tehran and suburbs of Tehran, Iran. Firstly, the thin blood smears were prepared and Giemsa staining method was conducted. Then, the samples were examined under oil immersion objective and 0.67% of them were observed infected with A. phagocytophilum. The DNA was extracted from blood samples using a DNA isolation kit (MBST, Iran), and MSP4 gene extraction was performed by Polymerase chain reaction (PCR) and nested-PCR. Finally, 2% of the samples were positive for A. phagocytophilum. The data were analyzed using SPSS software (version 19.0) and Chi-square test was performed. There was no significant relation between infection and age, as well as sex and ectoparasitic infestation (P>0.05). This article was a report of A. phagocytophilum infection in dogs and their potentials as host carriers of this important microorganism in Tehran, Iran. | ||
| کلیدواژه‌ها | ||
| Anaplasma phagocytophilum؛ Tick-borne؛ Dog؛ Tehran؛ Iran | ||
| مراجع | ||
|
Ahmadi-Hamedani, M., Khaki, Z., Rahbari, S., Kazemi, B., Bandehpour, M., 2009. Molecular identification of anaplasmosis in goats using a new PCR-RFLP method. Iran J Vet Res 10, 367-372.
Annen, K., Friedman, K., Eshoa, C., Horowitz, M., Gottschall, J., Straus, T., 2012. Two cases of transfusion-transmitted Anaplasma phagocytophilum. Am J Clin Pathol 137, 562-565.
Bagheri, M.M., 2016. Molecular detection of Anaplasma phagocytophilum in hard ticks of Khouzestan Province. Shahid Chamran University, Ahvaz.
Bakken, J.S., Folk, S.M., Paddock, C.D., Bloch, K.C., Krusell, A., Sexton, D.J., et al., 2006. Diagnosis and management of tickborne rickettsial diseases: Rocky Mountain spotted fever, ehrlichioses, and anaplasmosis—United States. MMWR Recomm Rep 55, 1-27.
Bashiribod, H., Kazemi, B., Eslami, G., Bigdeli, E., Bandehpour, M., Rahbarian, N., et al., 2004. First Molecular Detection of Anaplasma phagocytophilum in Ixodes ricinus Ticks in Iran. J Med Sci 4, 282-286.
Chen, S.M., Dumler, J.S., Bakken, J.S., Walker, D.H., 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J Clin Microbiol 32, 589-595.
De la Fuente, J., Massung, R.F., Wong, S.J., Chu, F.K., Lutz, H., Meli, M., et al., 2005. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J Clin Microbiol 43, 1309-1317.
Dreher, U.M., de la Fuente, J., Hofmann-Lehmann, R., Meli, M.L., Pusterla, N., Kocan, K.M., et al., 2005. Serologic cross-reactivity between Anaplasma marginale and Anaplasma phagocytophilum. Clin Diagn Lab Immunoly 12, 1177-1183.
Dumler, J.S., Barbet, A.F., Bekker, C.P., Dasch, G.A., Palmer, G.H., Ray, S.C., et al., 2001. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 51, 2145-2165.
Dumler, J.S., Choi, K.S., Garcia-Garcia, J.C., Barat, N.S., Scorpio, D.G., Garyu, J.W., et al., 2005. Human granulocytic anaplasmosis and Anaplasma phagocytophilum. Emerg Infect Dis 11, 1828-1834.
Ebani, V.V., Bertelloni, F., Turchi, B., Cerri, D., 2013. Serological and molecular survey of Anaplasma phagocytophilum in Italian hunting dogs. Ann Agric Environ Med 20, 289-292.
Ebani, V.V., Nardoni, S., Fognani, G., Mugnaini, L., Bertelloni, F., Rocchigiani, G., et al., 2015. Molecular detection of vector-borne bacteria and protozoa in healthy hunting dogs from Central Italy. Asian Pac J Trop Biomed 5, 108-112.
El Hamiani Khatat, S., Defauw, P., Marynissen, S., Van de Maele, I., van Dongen, A., Daminet, S., 2015. Exposure to Anaplasma phagocytophilum in two dogs in Belgium. Vlaams Diergeneeskd Tijdschr 84, 39-46.
Ewing, S.A., 1981. Transmission of Anaplasma marginale by arthropods. Proceedings of the 7th National Anaplasmosis Conference. Mississippi State University, Mississippi State, pp. 395-423.
Foil, L., 1989. Tabanids as vectors of disease agents. Parasitology today 5, 88-96.
Hosseini-Vasoukolaei, N., Oshaghi, M.A., Shayan, P., Vatandoost, H., Babamahmoudi, F., Yaghoobi-Ershadi, M.R., et al., 2014. Anaplasma infection in ticks, livestock and human in Ghaemshahr, Mazandaran Province, Iran. J Arthropod Borne Dis 8, 204.
Jalali, S., Khaki, Z., Kazemi, B., Bandehpour, M., Rahbari, S., Razi Jalali, M., et al., 2013. Molecular detection and identification of Anaplasma species in sheep from Ahvaz, Iran. Iran J Vet Res 14, 50-56.
Kocan, K.M., de la Fuente, J., Guglielmone, A.A., Melendez, R.D., 2003. Antigens and alternatives for control of Anaplasma marginale infection in cattle. Clin Microbiol Rev 16, 698-712.
Maazi, N., Malmasi, A.A., Nasiri, M., 2010. Seroprevalence of Anaplasma phagocytophilum in dogs in Tehran, Iran. European Veterinary Conference.1.
Melter, O., Stehlik, I., Kinska, H., Volfova, I., Ticha, V., Hulinska, D., 2007. Infection with Anaplasma phagocytophilum in a young dog: a case report. Vet Medicina-Praha- 52, 207.
Milner, J.M., van Beest, F.M., 2013. Ecological correlates of a tick-borne disease, Anaplasma phagocytophilum, in moose in southern Norway. Eur J Wildlife Res 59, 399-406.
Noaman, V., Bastani, D., 2016. Molecular study on infection rates of Anaplasma ovis and Anaplasma marginale in sheep and cattle in West-Azerbaijan province, Iran. Vet Res forum 7, 163-167.
Noaman, V., Shayan, P., 2009. Molecular detection of Anaplasma phagocytophilum in carrier cattle of Iran-first documented report. Iran J Microbiol 1, 37-42.
Pahlevan, S., 2016. Molecular detection of Anaplasma phagocytophilum in rural dogs around Ahvaz city. Shahid Chamran University, Ahvaz.
Potgieter, F.T., 1979. Epizootiology and control of anaplasmosis in South Africa. Journal of the South Afr Vet Assoc 50, 367-372.
Rassouli, M., Aghazamani, G., 2015. Retrospective Study of Tick-Borne Pathogens and Observation of Ehrlichia ewingii/Anaplasma phagocytophilum and Hemotropic Mycoplasma spp. in Dogs’ Blood Films.
Razmi, G.R., Dastjerdi, K., Hossieni, H., Naghibi, A., Barati, F., Aslani, M.R., 2006. An epidemiological study on Anaplasma infection in cattle, sheep, and goats in Mashhad Suburb, Khorasan Province, Iran. Ann NY Acad Sci 1078, 479-481.
Rikihisa, Y., 1991. The tribe Ehrlichieae and ehrlichial diseases. Clinical microbiology reviews 4, 286-308.
Robinson, M.T., Shaw, S.E., Morgan, E.R., 2009. Anaplasma phagocytophilum infection in a multi-species deer community in the New Forest, England. Eur J Wildl Res 55, 439-442.
Rymaszewska, A., Grenda, S., 2008. Bacteria of the genus Anaplasma–characteristics of Anaplasma and their vectors: a review. Vet Med 53, 573-584.
Salehi-Guilandeh, S., Sadeghi-Dehkordi, Z., Sadeghi-Nasab, A., Yousefi, A., 2018. Molecular detection of Anaplasma spp. in cattle of Talesh County, North of Iran. Bulg J Vet Med, Onlin first. DOI: 10.15547/bjvm.2135.
Santos, H.A., Thome, S.M., Baldani, C.D., Silva, C.B., Peixoto, M.P., Pires, M.S., et al., 2013. Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil. Parasites & vectors 6, 348.
Yousefi, A., Rahbari, S., Shayan, P., Sadeghi-dehkordi, Z., Bahonar, A., 2017a. Molecular detection of Anaplasma marginale and Anaplasma ovis in sheep and goat in west highland pasture of Iran. Asian Pac J Trop Biomed 7, 455-459.
Yousefi, A., Rahbari, S., Shayan, P., Sadeghi-dehkordi, Z., Bahonar, A., 2017b. Molecular evidence of Anaplasma phagocytophilum: an emerging tick-borne pathogen in domesticated small ruminant of Iran; first report. Comp Clin Pathol 26, 637-642.
Zhang, L., Liu, H., Xu, B., Zhang, Z., Jin, Y., Li, W., et al., 2014. Rural residents in China are at increased risk of exposure to tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis. BioMed Res Int 2014. | ||
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