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Molecular Detection of Anaplasma Phagocytophilum as a Zoonotic Agent in Owned and Stray Dogs in Tehran, Iran | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 74، شماره 1، خرداد 2019، صفحه 33-38 اصل مقاله (273.17 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.114893.1142 | ||
| نویسندگان | ||
| A. Yousefi* 1؛ M. R. Chaechi Nosrati2؛ A. Golmohammadi2؛ S. Azami3 | ||
| 1Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Microbiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Parasitology, Faculty of Paraveteriary Medicine, Bu-Ali Sina uiversity, Hamedan | ||
| چکیده | ||
| The genus Anaplasma is an obligated intracellular Rickettsia and among its species, Anaplasma phagocytophilum (A. phagocytophilum) is a zoonotic agent that infects host neutrophils. The aim of this study was molecular detection of A. phagocytophilum infection based on MSP4 gene in owned and stray dogs in Tehran, capital of Iran. One hundred and fifty blood samples were collected from dogs in Tehran and suburbs of Tehran, Iran. Firstly, the thin blood smears were prepared and Giemsa staining method was conducted. Then, the samples were examined under oil immersion objective and 0.67% of them were observed infected with A. phagocytophilum. The DNA was extracted from blood samples using a DNA isolation kit (MBST, Iran), and MSP4 gene extraction was performed by Polymerase chain reaction (PCR) and nested-PCR. Finally, 2% of the samples were positive for A. phagocytophilum. The data were analyzed using SPSS software (version 19.0) and Chi-square test was performed. There was no significant relation between infection and age, as well as sex and ectoparasitic infestation (P>0.05). This article was a report of A. phagocytophilum infection in dogs and their potentials as host carriers of this important microorganism in Tehran, Iran. | ||
| کلیدواژهها | ||
| Anaplasma phagocytophilum؛ Tick-borne؛ Dog؛ Tehran؛ Iran | ||
| مراجع | ||
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