| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,129 |
| تعداد مقالات | 34,788 |
| تعداد مشاهده مقاله | 50,060,789 |
| تعداد دریافت فایل اصل مقاله | 80,352,956 |
Designing a polytopic complex vaccine candidate against Gallibacterium anatis: an In-silico study | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 74، شماره 1، خرداد 2019، صفحه 7-20 اصل مقاله (729.49 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.109804.1118 | ||
| نویسندگان | ||
| S. Ataei* 1؛ M. M. Ranjbar2؛ N. Motamed1؛ S. Ataei Kachooei3؛ F. Amini Njafi4 | ||
| 1Department of Avian Bacterial Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran | ||
| 2Department of Animal Viral Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran | ||
| 3Department of Cellular Molecular Sciences, Faculty of Life Sciences, Kharazmi University, Karaj, Iran | ||
| 4R & D, Tose Kosar Group, Tehran, Iran | ||
| چکیده | ||
| The haemolytic biovar of Gallibacterium anatis (G. anatis) is responsible for urogenital, gastrointestinal, and respiratory diseases in chickens. There are numerous reports on the resistance of G. anatis to antibiotics and recurrence of the disease, which raise concerns about antimicrobial treatment efficiency. Vaccination has been considered as the most feasible procedure of prevention in high risk farms. Subunit vaccines containing immunogenic components can have practical protective value in preventive measures regarding the infection. The present study aimed to introduce a polytopic vaccine candidate based on epitope detection. All registered sequences of four immunogenic proteins, includig Flfa, GTxA, Gab_1309, and Gab_2348 were retrieved and directed for variational analysis. A vaccine isolate was selected for each protein and tested for B-cell epitope mapping using different tools. Furthermore, consensus selected immunogenic regions with special patterns fused together by flexible linkers were integrated into two constructs and checked for the best status of proteasomal cleavage sites, as well as hydropathy plot. Moreover, back translations, along with codon optimization were performed, and then some tags were added to the constructs. The selected consensus B-cell immunogenic epitopes were for 12656: AA114-181, 7990: AA114-181, Avicor: AA42-77, 134-197, and IPDH: 61-155 for Flfa protein, AA185-235, AA372-457, and AA807-941 for GtxA-N, AA260-305, AA340-400, and AA110-146 for Gab-1309, and AA125-AA175 for Gab-2348. Two suitable patterns of attachment were selected from the different fusion patterns of epitopes in B-cell polytopic vaccinal constructs. Finally, the examination of these constructs showed their effect and efficacy for immune system stimulation. Based on bioinformatics results, these immunogens could be utilized as potential candidates to develop polytopic protective vaccines and design diagnostic kits. | ||
| کلیدواژهها | ||
| Gallibacterium anatis؛ vaccine؛ Polytopic؛ In-silico | ||
| مراجع | ||
|
Bager, R.J., Kudirkiene, E., da Piedade, I., Seemann, T., Nielsen, T.K., Pors, S.E., et al., 2014. In silico prediction of Gallibacterium anatis pan-immunogens. Vet Res 45, 80.
Bager, R.J., Nesta, B., Pors, S.E., Soriani, M., Serino, L., Boyce, J.D., et al., 2013. The fimbrial protein FlfA from Gallibacterium anatis is a virulence factor and vaccine candidate. Infect Immun 81, 1964-1973.
Bojesen, A.M., Nielsen, O.L., Christensen, J.P., Bisgaard, M., 2004. In vivo studies of Gallibacterium anatis infection in chickens. Avian Pathol 33, 145-152.
Bojesen, A.M., Nielsen, S.S., Bisgaard, M., 2003. Prevalence and transmission of haemolytic Gallibacterium species in chicken production systems with different biosecurity levels. Avian Pathol 32, 503-510.
Christensen, H., Bisgaard, M., Bojesen, A.M., Mutters, R., Olsen, J.E., 2003. Genetic relationships among avian isolates classified as Pasteurella haemolytica, 'Actinobacillus salpingitidis' or Pasteurella anatis with proposal of Gallibacterium anatis gen. nov., comb. nov. and description of additional genomospecies within Gallibacterium gen. nov. International journal of systematic and evolutionary Microbiol 53, 275-287.
Garcia-Gomez, E., Vaca, S., Perez-Mendez, A., Ibarra-Caballero, J., Perez-Marquez, V., Tenorio, V.R., et al., 2005. Gallibacterium anatis-secreted metalloproteases degrade chicken IgG. Avian Pathol 34, 426-429.
Jones, K.H., Thornton, J.K., Zhang, Y., Mauel, M.J., 2013. A 5-year retrospective report of Gallibacterium anatis and Pasteurella multocida isolates from chickens in Mississippi. Poultry Sci 92, 3166-3171.
Kristensen, B.M., Frees, D., Bojesen, A.M., 2010. GtxA from Gallibacterium anatis, a cytolytic RTX-toxin with a novel domain organisation. Vet Res 41, 25.
Kristensen, B.M., Frees, D., Bojesen, A.M., 2011. Expression and secretion of the RTX-toxin GtxA among members of the genus Gallibacterium. Vet Microbiol 153, 116-123.
Mendoza, K., Zavaleta, A., Koga, Y., Rodriguez, J., Alvarado, A., 2014. Genetic variability of strains isolated from commercial Gallibacterium anatis birds of Peru with respiratory infections. J Vet Res Peru 25, 233-244.
Paudel, S., Alispahic, M., Liebhart, D., Hess, M., Hess, C., 2013. Assessing pathogenicity of Gallibacterium anatis in a natural infection model: the respiratory and reproductive tracts of chickens are targets for bacterial colonization. Avian Pathol 42, 527-535.
Pedersen, I.J., Pors, S.E., Bager Skjerning, R.J., Nielsen, S.S., Bojesen, A.M., 2015. Immunogenic and protective efficacy of recombinant protein GtxA-N against Gallibacterium anatis challenge in chickens. Avian Pathol 44, 386-391.
Pors, S.E., Skjerning, R.B., Flachs, E.M., Bojesen, A.M., 2016. Recombinant proteins from Gallibacterium anatis induces partial protection against heterologous challenge in egg-laying hens. Vet Res 47, 36.
Proctor, R.A., von Eiff, C., Kahl, B.C., Becker, K., McNamara, P., Herrmann, M., et al., 2006. Small colony variants: a pathogenic form of bacteria that facilitates persistent and recurrent infections. Nature reviews. Microbiology 4, 295-305.
Ranjbar, M., Mosavi Nasab, D., Ghelian Chian, A., Nazoktabar, A., Ahmadi, N., Khoshnevisan, R., et al., 2013. Immnoinformatics and epitope prediction methods dynamic science with promising achievements. Sci J Ilam Uni Med Sci 21, 300-309.
Ranjbar, M.M., Nayeb Ali, A., Ghorban, K., Ghalyanchi Langeroudi, A., Dadmanesh, M., Amini, H.-R., et al., 2015. Immnoinformatics: novel view in understanding of immune system function, databases and prediction of immunogenic epitopes. Koomesh, 18-26.
Singh, S., Singh, B., Sinha, D., Vinodh, K., Prasanna, V., Monika, B., et al., 2016. Gallibacterium anatis: an emerging pathogen of poultry birds and domiciled birds. J Vet Sci Technol 7.
Wang, C., Robles, F., Ramirez, S., Riber, A.B., Bojesen, A.M., 2016. Culture-independent identification and quantification of Gallibacterium anatis (G. anatis) by real-time quantitative PCR. Avian Pathol 45, 538-544.
Zepeda, A., Ramirez, S., Vega, V., Morales, V., Talavera, M., Salgado-Miranda, C., et al., 2009. Hemagglutinating activity of Gallibacterium strains. Avian Dis 53, 115-118. | ||
|
آمار تعداد مشاهده مقاله: 2,791 تعداد دریافت فایل اصل مقاله: 3,098 |
||