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تغییرات تنوع اکوتیپی جمعیتهای گونه بلندمازو (Quercus castaneifolia) در طبقات ارتفاعی مختلف جنگل (مطالعه موردی: جنگلهای شرق استان گلستان) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 12، دوره 26، شماره 2 - شماره پیاپی 52، دی 1397، صفحه 279-291 اصل مقاله (336.27 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.117966 | ||
| نویسندگان | ||
| اعظم احمدی مزرعچه1؛ داوود آزادفر* 2؛ زهره سعیدی3 | ||
| 1دانشآموخته کارشناسی ارشد جنگلشناسی و اکولوژی جنگل، دانشکده علوم جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 2نویسنده و مسئول مکاتبات، دانشیار، بیوتکنولوژی جنگل، دانشکده علوم جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، پست الکترونیکی: azadfar.d@gmail.com | ||
| 3دانشآموخته دکترای علوم جنگل، دانشکده علوم جنگل، دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1397.2.279.52.26.1578.41 آگاهی و شناخت تنوع ژنتیکی گونهها برای سازگاری با تغییرات محیطی و بقای طولانی مدت آنها و همچنین مدیریت حفاظت منابع ژنتیکی جنگل بسیار مهم و حیاتی است و باید برای گونههای مهم صنعتی یا اکولوژیکی در اولویت مطالعه قرار گیرد. هدف از این پژوهش بررسی تغییرات تنوع اکوتیپی جمعیتهای بلندمازو در طبقههای ارتفاعی (ارتفاع پایینی، میانی و بالایی) شرق استان گلستان، با استفاده از فعالیت کیفی نشانگر بیوشیمیایی پراکسیداز شاخههای دوساله در شش جمعیت مختلف بود. مطالعه کیفی نشانگر پراکسیداز با استفاده از الکتروفورز عمودی و بهروش الکتروفورز ژل پلی اکریل آمید (PAGE) انجام شد. نتایج نشان داد که جمعیتهای طبقههای ارتفاعی از نظر میزان تنوع در هر دو رویشگاه متفاوت بوده و جمعیت بالایی رویشگاه علیآباد کتول دارای بیشترین تنوع بودند. بنابراین برای افزایش تنوع باید ضمن تعیین پایههای شاخص اکوتیپی در محوطههای بذرگیری هر منطقه و کمک به تجدید حیات طبیعی آنها، نسبت به جریان ژنی از طریق بذر پایههای شاخص جمعیتهای همجوار با شرایط اکولوژیک مشابه همراه با کنترل تعداد پایههای شاخص انتخابی با توجه به نیاز افزایش تنوع در هر رویشگاه اقدام نمود. | ||
| کلیدواژهها | ||
| استان گلستان؛ بلندمازو؛ تنوع اکوتیپی؛ طبقه ارتفاعی؛ نشانگر پراکسیداز | ||
| عنوان مقاله [English] | ||
| Ecotypic diversity alteration of Quercus castaneifolia populations in different elevation classes of forest (case study: East of Golestan province) | ||
| نویسندگان [English] | ||
| A. Ahmadi1؛ D. Azadfar2؛ Z. Saeedi3 | ||
| 1M.Sc., Graduated Student of Silviculture and Forest Ecology, Forest Science Faculty, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran. | ||
| 2Corresponding Author, Assoc. Prof., in Forest Biotechnology, Forest Science Faculty, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran, Email: azadfar.d@gmail.com | ||
| 3Ph.D. in Forest Sceince, Forest Science Faculty, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, I.R.Iran. | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1397.2.279.52.26.1578.41 Knowledge and understanding the genetic diversity of species is very important for adaptation to environmental changes and their long-term survival and also for the management of forest genetic resources conservation. Therefore, study of industrial or ecologically important species should be done in order of priority. The aim of this research was exploring the ecotypic diversity changes of Quercus castaneifolia populations in elevation classes of Golestan province (low, middle, and upper elevation) by the qualitative study of peroxidase biochemical marker in the biennial branches of six different populations. Qualitative study of peroxidase marker was done by vertical electrophoresis and PAGE method (Poly Acrylamide Gel Electrophoresis). Based on the results, populations of altitudinal classes were different regarding amount of diversity and ecotypes were separable from each other. Therefore, index bases in the seed production areas should be determined and their natural regeneration should be increased and gene flow should be improved through the seeds of index bases of the adjacent populations with similar ecological conditions with controlling the number of selected index bases considering the increased diversity in each habitat. | ||
| کلیدواژهها [English] | ||
| Ecotype diversity, elevation classes, Golestan Province, Quercus castaneifolia, peroxidase marker | ||
| مراجع | ||
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