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مطالعه اثر تنوع ژنتیکی در پاسخ مورفو- فیزیولوژیکی و آنتیاکسیدانی گونههای مختلف آژیلوپس به تنش خشکی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 10، دوره 26، شماره 2 - شماره پیاپی 52، دی 1397، صفحه 254-267 اصل مقاله (508.48 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.117964 | ||
| نویسندگان | ||
| صدیقه فابریکی اورنگ* 1؛ بتول شهیدی2 | ||
| 1استادیار، هیات علمی دانشگاه بینالمللی امام خمینی (ره)، قزوین، ایران | ||
| 2دانشجوی کارشناسی ارشد، دانشکده کشاورزی و منابع طبیعی، دانشگاه بینالمللی امام خمینی (ره)، قزوین | ||
| چکیده | ||
| DOR: 98.1000/1735-0891.1397.2.254.52.26.1605.1610 این آزمایش بهمنظور بررسی اثر تنش خشکی بر محتوای رنگیزههای فتوسنتزی، وزن ریشه و اندامهوایی، محتوای پروتئین کل و فعالیت آنزیمهای آنتیاکسیدان آنزیمی و غیرآنزیمی در شش گونه مختلف از جنس Aegilops بههمراه دو رقم گندم نان مقاوم و حساس به تنش خشکی بهعنوان شاهد، در قالب فاکتوریل بر پایه طرح بلوکهای کامل تصادفی با سه تکرار اجرا شد. تیمارهای تنش خشکی در سه سطح عدم تنش (FC=100%)، تنش متوسط (FC=50%) و تنش شدید (FC=25%) اعمال شد. گونههای Ae. caudata، Ae. triuncialis وAe. cylindricaاز نظر صفات وزن تر و خشک ریشه در شرایط تنش نسبت به رقم شاهد مقاوم سیروان در رتبه بالاتری قرار گرفتند. بیشترین مقدار کلروفیل کل در شرایط تنش متوسط در Ae. cylindrica و Ae. umbellulata و در تنش شدید در گونه Ae. caudata مشاهده شد. در تمام گونههای مورد مطالعه بهجز گونه Ae. umbellulata در اثر تنش خشکی میزان کاروتنوئید روند کاهشی نشان داد و گونه Ae. cylindrica نسبت به سایر گونهها از بیشترین مقدار برخوردار بود. بالاترین مقدار آنزیم سوپراکسید دیسموتاز در خشکی شدید در گونه Ae. crassa و رقم مقاوم Sirvanو کمترین مقدار آن در گونه Ae. neglecta مشاهده گردید. بیشترین آنزیم آسکوربات پراکسیداز در خشکی شدید در گونههای Ae. triuncialis و Ae. caudata و کمترین مقدار آن در گونه Ae. neglecta مشاهده شد. بالاترین مقدار آنزیم گایاکول پراکسیداز در گونههای Ae. triuncialis، Ae. caudata و رقم مقاوم Sirvan در تنش خشکی مشاهده شد و گونههای Ae. triuncialis، Ae. caudata و Ae. crassa بیشترین و گونه Ae. umbellulata و رقم حساس Darya کمترین مقدار آنزیم کاتالاز را در خشکی شدید داشتند. در جمعبندی کلی، نظر به اینکه سه گونه Ae. caudata، Ae. Triuncialis و Ae. Cylindrica از نظر اغلب صفات مرتبط با تحمل خشکی ازجمله حجم ریشه، کارتنوئید و آنزیمهای CAT، GPX و APX بالاترین مقدار را داشتند و هر سه گونه در ژنوم CC مشترک بودند، ازاینرو بهنظر میرسد ژنوم CC بتواند ظرفیت بالایی در برنامههای اصلاحی آینده گندم برای افزایش مقاومت به خشکی داشته باشد. | ||
| کلیدواژهها | ||
| آژیلوپس؛ آنتیاکسیدان؛ تنش خشکی؛ رنگیزههای فتوسنتزی | ||
| عنوان مقاله [English] | ||
| Evaluation of genetic diversity effects on morpho-physiological and antioxidant responses in different species of Aegilops under drought stress | ||
| نویسندگان [English] | ||
| S. Fabriki-Ourang1؛ B. Shahidi2 | ||
| 1Assistant Professor, Department of Production and Plant Breeding, Imam Khomeini International University, Qazvin, Iran | ||
| 2M.Sc. Student, Imam Khomeini International University, Qazvin, I.R.Iran | ||
| چکیده [English] | ||
| DOR: 98.1000/1735-0891.1397.2.254.52.26.1605.1610 This experiment was conducted to investigate the effect of drought stress on photosynthetic pigments contents, biomass of roots and shoots, total protein content and antioxidant enzymes activity and non-enzymatic antioxidant of six species of Aegilops genus along with two drought resistant and susceptible wheat cultivars as checks in a factorial based on randomized complete block design with three replications. Drought stress treatments were applied at three levels of non-stress (FC=100%), moderate stress (FC=50%) and severe stress (FC=25%) conditions. Ae. caudata, Ae. triuncialis and Ae. cylindrica were ranked higher than resistance cultivar, Sirvan, as check in term of root fresh and dry weights under stresses. The highest total chlorophyll content was related to Ae. cylindrica, Ae. umbellulata and Ae. caudata in stresses. Carotenoid showed decreasing trend in all studied species except for Ae. umbellulata, and Ae cylindrica had the highest amount at both stress and non- stress conditions. The highest SOD under severe stress was observed in Ae. crassa and Sirvan as resistant cultivar. Maximum APX under severe stress was observed in Ae. triuncialis and Ae. caudata and the lowest activity was in Ae. neglecta. The highest level of GPX was observed in Ae. triuncialis, Ae. caudata and Sirvan cultivar under drought stress. Also the maximum CAT enzyme under severe stress was observed in Ae. triuncialis, Ae. caudata and Ae. crassa, respectively, and Ae. umbellulata and Darya cultivar had the lowest amount of CAT enzyme. In conclusion, because the three species Ae. caudata, Ae. triuncialis and Ae. cylindrica had the highest amounts for the traits associated with increased drought tolerance, including root volume, carotenoid, CAT, GPX and APX enzymes, and also these species were same in having CC genome. Therefore, it seems that the CC genome could have a high potential for future wheat breeding programs in increasing of drought tolerance. | ||
| کلیدواژهها [English] | ||
| Aegiliops, Antioxidants, Drought Stress, photosynthetic pigments | ||
| مراجع | ||
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