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pahlavan yali, morteza, Jalili, Hassan, noroozi, mostafa, Morady, yazdan, saba, farkhondeh. (1398). Optimization of culture condition for growth of the Aurantiochytrium sp. shy, isolated from Persian Gulf. سامانه مدیریت نشریات علمی, 18(4), 671-656. doi: 10.22092/ijfs.2018.117491
morteza pahlavan yali; Hassan Jalili; mostafa noroozi; yazdan Morady; farkhondeh saba. "Optimization of culture condition for growth of the Aurantiochytrium sp. shy, isolated from Persian Gulf". سامانه مدیریت نشریات علمی, 18, 4, 1398, 671-656. doi: 10.22092/ijfs.2018.117491
pahlavan yali, morteza, Jalili, Hassan, noroozi, mostafa, Morady, yazdan, saba, farkhondeh. (1398). 'Optimization of culture condition for growth of the Aurantiochytrium sp. shy, isolated from Persian Gulf', سامانه مدیریت نشریات علمی, 18(4), pp. 671-656. doi: 10.22092/ijfs.2018.117491
pahlavan yali, morteza, Jalili, Hassan, noroozi, mostafa, Morady, yazdan, saba, farkhondeh. Optimization of culture condition for growth of the Aurantiochytrium sp. shy, isolated from Persian Gulf. سامانه مدیریت نشریات علمی, 1398; 18(4): 671-656. doi: 10.22092/ijfs.2018.117491

Optimization of culture condition for growth of the Aurantiochytrium sp. shy, isolated from Persian Gulf

Iranian Journal of Fisheries Sciences
مقاله 68، دوره 18، شماره 4، دی 2019، صفحه 671-656    اصل مقاله (396.78 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.117491
نویسندگان
morteza pahlavan yali1؛ Hassan Jalili* 2؛ mostafa noroozi3؛ yazdan Morady4؛ farkhondeh saba3
1ph.d student at iranian fisheries research institute
2Life science engineering Dep. Faculty of New Sciences and Technologies-University of Tehran
3Biotechnology Dep. Faculty of Biological sciences-Alzahra University
4Seafood Processing & Biotechnological Dep. Iranian Fisheries sciences Research Institue
چکیده
In this study, a native microalgae strain with a high capability of DHA production has been isolated and purified from Mangrove shores in the Persian Gulf. Morphological and molecular identification based on 18S rRNA gene showed that the isolated strain belongs to Aurantiochytrium genus. In order to optimize the growth of this strain, various environmental factors were examined, including temperature(15, 20, 25, 30 and 35°C), salinity (10, 30 and 50 g/L) and different concentrations of nitrogen sources of Yeast extract, Peptone, Meat Extract and Monosodium glutamate, each of which being tested with 10 g/L concentrations. The findings show that the optimal growth conditions occur in 25-30°, pH 6 and presence of Meat Extract as a nitrogen source. In these conditions, the cellular biomass weight, fatty acids and DHA amounts were obtained as 7.34, 2.25 and 0.4 g/L, respectively. These results imply that the new isolated strain of Aurantiochytrium sp.shy is suitable for lipid production.
 
کلیدواژه‌ها
Isolation؛ Aurantiochytrium sp؛ optimization؛ biomass؛ Microalgae؛ Heterotrophic
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