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شناسایی مولکولی و ریختشناسی دو گونه از کنههای خانوادهPachylaelapidae Berlese (Acari: Mesostigmata) در استان گیلان | ||
| نامه انجمن حشره شناسی ایران | ||
| مقاله 10، دوره 38، شماره 1 - شماره پیاپی 73، خرداد 1397، صفحه 117-127 اصل مقاله (720.02 K) | ||
| نوع مقاله: مقاله کامل، فارسی | ||
| شناسه دیجیتال (DOI): 10.22117/jesi.2018.122186.1233 | ||
| نویسندگان | ||
| رضا حسینی* 1؛ سمانه مجاهد2؛ جلیل حاجی زاده3؛ علی احدیت4 | ||
| 1گروه گیاهپزشکی ، داتشکده ی علوم کشاورزی، دانشگاه گیلان، رشت ، ایران | ||
| 2گروه گیاهپزشکی، دانشکده ی علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 3گروه گیاهپزشکی، دانشکده‌ی علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 4گروه حشره‌شناسی کشاورزی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران | ||
| چکیده | ||
| کنههای خانوادهBerlese, 1913 Pachylaelapidae کنههایی شکارگر با زندگی آزاد هستند و از بندپایان و نماتودهای خاکزی تغذیه میکنند. از آنجا که تعداد قابل توجهی از کنههای این خانواده شباهتهای ریختشناسی به هم داشته و همچنین توصیفکنندگان تاکسونهای جدید به برخی از ویژگیهای ریختشناسی استناد کردهاند که وجه تمایز کافی برای افتراق آنها از تاکسونهای نزدیک را ندارند، شناسایی برخی از این کنهها دشوار است. در این موارد استفاده از روشهای نوین شناسایی میتواند در این زمینه راهگشا باشد. بنابراین، در این بررسی تلاش شده تا همراه با شناسایی بر مبنای ویژگیهای ریختشناسی و اندازهای (شناسایی مورفومتریک)، از روش مولکولی بر پایهی توالییابی ناحیه ژنی ITS (Internal Transcribed Spacer) برای شناسایی دو گونه Olopachys compositus Koroleva, 1976 و Olopachys caucasicus Koroleva, 1976 استفاده شود. به این منظور پس از استخراج DNA، با استفاده از آغازگر مناسب ناحیهی ITS این دو گونه تکثیر و توالییابی شدند و توالیهای به دست آمده در بانک ژن ثبت شد. پس از همردیفسازی توالیها، میزان اختلاف ژنتیکی بین آنها 3/2 درصدمحاسبه شد. در نتیجه این بررسیها، علاوه بر تفکیک دو گونه O. compositus و O. caucasicus بر اساس اختلافات ریختی، روش مولکولی نیز به خوبی اختلاف این دو گونه را تأیید کرد. | ||
| کلیدواژه‌ها | ||
| کنه؛ ITS؛ توالییابی؛ Pachylaelapidae؛ Olopachys | ||
| عنوان مقاله [English] | ||
| Molecular and morphological identification of two species of mites of the family Pachylaelapidae Berlese (Acari: Mesostigmata) in Guilan province | ||
| نویسندگان [English] | ||
| R. Hosseini1؛ Samaneh Mojahed2؛ J. Hajizadeh3؛ Ali Ahadiyat4 | ||
| 1Department of Plant protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 2Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht | ||
| 3Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran | ||
| 4Department of Agricultural Entomology, Faculty of Agriculture And Natural Resources, Islamic Azad University, Branch of Sciences and Researches, Tehran, Iran | ||
| چکیده [English] | ||
| Mites of the family Pachylaelapidae Berlese, 1913 are a group of predatory mites with free-living species, feeding on arthropods and soil-inhabiting nematodes. Identification of these mites is difficult, because of morphological similarities in a significant number of species and also the fact that new taxons descriptors have referred to some morphological features that have not sufficient distinction criteria to differentiate them from close taxons. Therefore, the use of new identification methods can be helpful in this context. In the present study, along with identification, based on morphological characteristics and size (morphometric identification), we used a molecular method, based on the sequencing of the internal transcribed spacer (ITS) gene to identify two species Olopachys compositus Koroleva, 1976 and Olopachys caucasicus Koroleva, 1976. After DNA extraction, ITS region were amplified and sequenced, using the appropriate primers and afterward the sequences were recorded in GeneBank. Alignment of sequences was performed and the genetic differences between species was calculated as 2.3 percent. As a result of these investigations, in addition to the separation of the two species O. compositus and O. caucasicus based on morphological differences, the molecular method also well confirmed the difference between these two species. | ||
| کلیدواژه‌ها [English] | ||
| Mite, ITS, Sequencing, Olopachys, Pachylaelapidae | ||
| مراجع | ||
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