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شناسایی مولکولی گونه های نوزما در زنبورستان های استان کردستان | ||
| علوم و فنون زنبور عسل ایران | ||
| مقاله 1، دوره 09، شماره 16، شهریور 1397، صفحه 2-11 اصل مقاله (477.38 K) | ||
| شناسه دیجیتال (DOI): 10.22092/hbsj.2018.117152 | ||
| نویسندگان | ||
| محمد خضری* 1؛ مجتبی محرمی2؛ حسین مدیرروستا3؛ مریم ترکمن4؛ صالح صالحی1؛ بابک رخزاد1؛ هومن خانبابایی1 | ||
| 1مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی کردستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، سنندج، ایران | ||
| 2بخش تشخیص و تحقیق بیماری های زنبورعسل،کرم ابریشم و حیات وحش، موسسه تحقیقات واکسن وسرم سازی رازی، مؤسسه تحقیقات، آموزش و ترویج کشاورزی،کرج، ایران | ||
| 3بخش تشخیص و تحقیق بیماری های زنبورعسل،کرم ابریشم و حیات وحش، موسسه تحقیقات واکسن وسرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| 4بخش تشخیص و تحقیق بیماری های زنبورعسل،کرم ابریشم و حیات وحش، موسسه تحقیقات واکسن وسرم سازی رازی، کرج، ایران | ||
| چکیده | ||
| میکروسپوریدیاها انگلهای فرصت طلب داخل سلولی اسپورزا هستند که بطور طبیعی قادر به آلوده نمودن کلیه بی مهره گان بالاخص حشرات و همچنین مهره داران از جمله انسان می باشند. نوزموزیس یکی از مهمترین و شایعترین بیماری های زنبورعسل بالغ در سراسر جهان است. با توجه به این که گونه های مختلفی از نوزما درزنبورعسل موجب بیماری می گردند و شناسایی گونه مسبب بیماری باعث تدوین استراتژی مناسب مبارزه می گردد لذا به منظور شناسایی مولکولی عامل بیماری نوزموزیس در استان کردستان اقدام به نمونه برداری از100 زنبورستان استان گردید و سپس با استفاده از روشPCR اقدام به شناسایی عامل این بیماری در نمونه ها گردید. نتایج بدست آمده از این تحقیق نشان داد که تنها گونه نوزمای شناسایی شده در استان کردستان به روش مولکولی گونه نوزما سرانه می باشد و در نمونه های آزمایش شده اثری از دیگر گونه های نوزما مشاهده نشد، لذا با توجه به نتایج بدست آمده می توان اذعان نمود که محتملاً گونه نوزما سرانه عامل بیماری نوزموزیس درزنبورستان های استان کردستان می باشد. | ||
| کلیدواژه‌ها | ||
| نوزما سرانه؛ کردستان؛ شناسایی مولکولی؛ نوزموزیس | ||
| عنوان مقاله [English] | ||
| Molecular identification of Nosema species in the apiary of Kurdistan province of Iran | ||
| نویسندگان [English] | ||
| Mohammad Khezri1؛ Mojtaba Moharrami2؛ Hosein Modirrousta3؛ Mariam Torkaman4؛ Saleh Salehi1؛ Babak Rokhzad1؛ Homan Khanbabaie1 | ||
| 1Veterinary Research Department, Kurdistan , Agricultural Research Education and Extension (AREEO), Sanandaj, Iran | ||
| 2Department of Honey Bee, Silk Worm and Wildlife Research Diseases, Razi Vaccine and Serum Research Institute,Agricultural Research Education and Extension Organization(AREEO) , Karaj, Iran | ||
| 3Department of Honey Bee, Silk Worm and Wildlife Research Diseases, Razi Vaccine and Serum Research Institute,Agricultural Research Education and Extension Organization (AREEO) , Karaj, Iran | ||
| 4Department of Honey Bee, Silk Worm and Wildlife Research Diseases, Razi Vaccine and Serum Research Institute, Karaj, Iran | ||
| چکیده [English] | ||
| Nosema disease is the most important adult bee disease but is mostly by beekeepers as there are no characteristic obvious symptoms. Hence, Nosema disease is also referred to as "the silent killer". The aim of the present study was to determine the molecular identification of Nosema spices in Kurdistan province of Iran. 100 samples were collected from apiaries (870 hives) of Kurdistan. Samples were examined by a light microscope and polymerase chain reaction (PCR) assay. The light microscope was used for determination of the presence of Nosema spores in all of the collected samples. PCR assay based on 16S ribosomal RNA has been used to differentiate between Nosema apis and Nosema ceranae. Only Nosema ceranae was found in all samples, indicating that this species present in Kurdistan | ||
| کلیدواژه‌ها [English] | ||
| Nosema ceranae, Kurdistan, molecular identification, Nosemosis | ||
| مراجع | ||
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