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بررسی تغییرات آنزیم پراکسیداز و پلی فنل اکسیداز در ارقام حساس و متحمل خیار نسبت به نماتد مولد گره ریشه، در اثر قارچ مایکوریز | ||
| آفات و بیماری‌های گیاهی | ||
| مقاله 1، دوره 87، شماره 1 - شماره پیاپی 108، شهریور 1398، صفحه 1-11 اصل مقاله (626.69 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2018.120132.1202 | ||
| نویسندگان | ||
| آمنه حسینی خواه چوشلی1؛ سعید رضائی2؛ سالار جمالی* 3؛ حمیدرضا زمانی زاده4؛ فرهاد رجالی5 | ||
| 1گروه بیماری‌شناسی گیاهی دانشکده کشاورزی و منابع طبیعی دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران. | ||
| 2گروه بیماری شناسی گیاهی دانشکده کشاورزی و منابع طبیعی واحد علوم و تحقیقات دانشگاه آزاد اسلامی تهران، ایران | ||
| 3گروه گیاهپزشکی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران. | ||
| 4دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، دانشکده کشاورزی و منابع طبیعی | ||
| 5موسسه تحقیقات خاک و آب کرج | ||
| چکیده | ||
| هدف از این تحقیق، مقایسه تأثیر قارچ مایکوریز آربوسکولار Funneliformis mosseaeبر فعالیت آنزیمهای دفاعی تولید شده در اثر حمله نماتد مولد گره Meloidogyne incognita به ریشه خیار ارقام متحمل (سوپردومینوس) و حساس (دانیتو) بوده است. به این منظور آزمایشی در گلخانه با چهار تیمار و چهار تکرار در قالب طرح کاملاً تصادفی (طرح خرد شده در زمان) انجام گرفت. ابتدا گیاهچههای خیار در خاک سترون پرورش یافت. به تیمارهای حاوی مایکوریز میزان 75 گرم زادمایه قارچ در هر کیلوگرم خاک اضافه گردید. 45 روز بعد از مایه زنی قارچ مایکوریز، تعداد 1500 عدد لارو سن دوم در هر کیلوگرم خاک اضافه شد. تغییرات کمی آنزیمهای پراکسیداز و پلی فنل اکسیداز در روزهای دوم، چهارم، ششم و هشتم بعد از مایه زنی اندازه‌گیری شد. نتایج نشان داد که در تیمار مایه زنی شده با نماتد، میزان فعالیت آنزیمهای مذکور به نسبت گیاه شاهد افزایش یافت. در تیمار قارچ مایکوریز به تنهایی، میانگین فعالیت آنزیمها در تمام زمان‌ها، بالاتر از میانگین شاهد و تیمار نماتد بود. در هر دو رقم، در تیمار نماتد به همراه قارچ مایکوریز، فعالیت آنزیمهای مذکور به طور قابل ملاحظه‌ای افزایش یافت. به طور کلی، میزان فعالیت آنزیمهای پراکسیداز و پلی فنل اکسیداز در رقم متحمل به طور قابل ملاحظه‌ای بیش‌تر از رقم حساس در همان تیمارها و روزهای اندازه‌گیری بود که میتواند با نقش این آنزیمها در افزایش تحمل نسبت به نماتد ارتباط داشته باشد. تأثیر قارچ مایکوریز در افزایش مقادیر آنزیمهای دفاعی در تیمارهای مورد بررسی از دیگر نتایج این تحقیق به شمار میرود. | ||
| کلیدواژه‌ها | ||
| پراکسیداز؛ پلی فنل اکسیداز؛ Funneliformis mosseae؛ Meloidogyne incognita؛ Cucumis sativus | ||
| عنوان مقاله [English] | ||
| Investigation of peroxidase and polyphenol oxidase enzymes quantitative changes in sensitive and tolerant cucumber to root knot nematode, due to mycorrhizal fungus | ||
| نویسندگان [English] | ||
| amaneh hosseinikhah choshali1؛ Saeed Rezaee2؛ Salar Jamali3؛ hamidreza zamanizadeh4؛ farhad rejali5 | ||
| 11Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
| 2Department of Plant Pathology College of Agriculture and Natural Resources Science and Research Branch Islamic Azad University Tehran, Iran | ||
| 3Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran. | ||
| 41Department of Plant Pathology, College of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
| 5Soil and Water Research Institute, Tehrān, Iran. | ||
| چکیده [English] | ||
| The The aim of this study was to compare the effect of Funneliformis mosseae mycorrhizal fungus on the activity of defense enzymes produced by Meloidogyne incognita attack on roots of tolerant and sensitive cucumber cultivars. For this purpose, an experiment was conducted in a greenhouse with four treatments and four replications in a completely randomized design (split design in time). Cucumber seedlings were planted in sterile soil. Mycorrhizal treatments were added to 75 grams inoculum per kilogram soil. 45 days after F. mosseae inoculation, 1500 J2 were added per each kilogram soil. The quantitative activity of peroxidase and polyphenol oxidase enzymes was measured after six and eigh days after inoculation. The results showed that the activity of the enzymes in inoculated with nematode increased in comparison with the control plants. In the inoculated with AMF alone, the average activity of enzymes in different days of measurement was higher than control and nematode treatment. In both cultivars, the activity of these enzymes increased significantly in nematode with mycorrhizal fungus. Totally, the activity of peroxidase and polyphenol oxidase enzymes in tolerant cultivar was significantly higher than sensitive in the same treatments and days of measurement which can be related to these enzymes roll in increasing tolerance to the nematode. Effect of mycorrhizal fungus on increasing the amount of defense enzymes in the treatments are considered as other results of this research. | ||
| کلیدواژه‌ها [English] | ||
| Cucumis sativus, Funneliformis mosseae, Meloidogyne incognita, Peroxidase, Polyphenol oxidase | ||
| مراجع | ||
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