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بررسی تأثیر پیش تیمار بذر با آب و پلی آمین ها بر بنیه و مقاومت به سرمای جو رقم یوسف برداشت شده با رطوبت های مختلف | ||
| علوم و فناوری بذر ایران | ||
| مقاله 17، دوره 6، شماره 2 - شماره پیاپی 12، آبان 1396، صفحه 213-228 اصل مقاله (869.71 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2018.116773 | ||
| نویسندگان | ||
| لیلا یاری* 1؛ محمد صدقی2؛ آیدین حمیدی3؛ رئوف سید شریفی4 | ||
| 1دانش آموخته مقطع دکتری رشته علوم و تکنولوژ ی بذر دانشگاه محقق اردبیلی | ||
| 2گروه زراعت و اصلاح نباتات دانشکده کشاورزی و منابع طبیعی دانشگاه محقق اردبیلی | ||
| 3دانشیار پژوهش/مؤسسه تحقیقات ثبت و گواهی بذر و نهال،کرج، البرز، ایران | ||
| 4دانشیار/گروه زراعت و اصلاح نباتات، دانشکده کشاورزی و منابع طبیعی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| چکیده | ||
| به منظور بررسی تأثیر رطوبت زمان برداشت و تیمارهای بذری بر وضعیت بنیه بذر و مقاومت به سرمای گیاهچه جو در شرایط کنترل شده، آزمایشی در مؤسسه تحقیقات ثبت و گواهی بذر و نهال کرج بصورت فاکتوریل با طرح پایه کاملاً تصادفی با چهار تکرار در سال93-1392 انجام گرفت. تیمارها عبارت بودند از رطوبت بذر در زمان برداشت در سه سطح 14، 16 و 18% و پیش تیمار بذر در پنج سطح شامل سه نوع محلول پلی آمین پوترسین، اسپرمیدین، اسپرمین، آب وعدم پیش تیمار بذر به عنوان شاهد بودند. براساس نتایج پیش تیمارکردن بذرها با پلی آمینها موجب بهبود بنیه بذر، کاهش نشت الکترولیت، افزایش محتوای پرولین گیاهچه ها و افزایش رشد گیاهچه های حاصل از بذرهای برداشت شده در هر سه سطح رطوبتی 14، 16 و 18 % در مقایسه با شاهد( عدم پیش تیمار) گردید. بعلاوه تیمارپرایمینگ بذرها با آب نیز باعث کاهش در نشت الکترولیت ها، افزایش وزن خشک بخش هوایی گاهچه ها، وزن خشک ریشه و طول ساقه بعد از شرایط تنش سرما گردید. حداکثر میزان پرولین و وزن خشک بخش هوایی گیاهچه ها بعد از شرایط تنش سرما، از تیمار پوترسین (Put) و حداقل نشت الکترولیتها نیز برای تیمار Put ثبت گردید. همچنین بذرهایی که زودتر برداشت شده بودند (با رطوبت 18 درصد) از بنیه بهتر، وزن خشک اندام هوایی بالاتر و نشت الکترولیت کمتر درشرایط سرما برخوردار بودند. به طورکلی برداشت بذرها بارطوبت در محدوده 17% تا18%، انبار نمودن آنها در شرایط مناسب و سپس استفاده از تیمارهای بذری آب و PAs(یک ماه قبل از کاشت)، بویژه Put برای بهبود در بنیه بذر و مقابله گیاهچه ها با شرایط تنش سرما قابل توصیه است . | ||
| کلیدواژه‌ها | ||
| آب؛ پرولین؛ پلی آمین؛ رشد گیاهچه؛ رطوبت زمان برداشت؛ نشت الکترولیت | ||
| عنوان مقاله [English] | ||
| Effect of water and Poly Amines treatment on barley (Hordeum vulgare L.) c.v Usef seedling vigour and chilling tolerance at harvesting in different seed moisture content | ||
| نویسندگان [English] | ||
| L. Yari1؛ M. Sedghi2؛ A. Hamidi3؛ R. Seyed Sharifi4 | ||
| 1Ph.D., graduated in Seed Science and Technology, University of Mohaghegh Ardabili | ||
| 2University of Mohaghegh Ardabili, Faculty of Agriculture and Natural Resources, Department of Agronomy and Plant Breeding | ||
| 3Assoc. Prof./ Seed and Plant Certification and Registration Institute (SPCRI), Karaj-IRAN | ||
| 4Assoc. Prof./ Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| چکیده [English] | ||
| This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the seed and Plant certification and Registration Research Institute in 2013 -2014. The seeds were hand harvested at three initial moisture contents including 18, 16, and 14% wet weight basis .The seed samples then were sealed in polythene bags and stored in conditioned storage and equilibrium for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, Seeds dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again until one month. Experimental units were arranged as factorial in a completely randomized design with four replications after treat. The results indicated that Mc18% improved the germination percentage (GP) and seedling vigour compared to Mc 16 and 14%. The electrolyte leakage decreased in seeds harvested by Mc 18% when compared with Mc16 and Mc 14.0%. Also seed treatments significantly increased the seed viability. Likewise, in chilling stress condition maximum proline content and areal dry weight were obtained from Put treat, whereas the lowest electrolyte leakage was recorded for Put treat. Meanwhile, interaction between seed moisture content ×PAs treatment in cold condition significantly affected on plumule length and radicle dry weight, statistically maximum plumule length and radicle dry weight were recorded from Put ×18.0% mc. Also seed priming with water had positive effect on seedling growth after chilling stress. Generally, the effectiveness of PAs on improving chilling tolerance and increasing seedling vigor was more pronounced in Put treatment along with sample with 18.0% mc. | ||
| کلیدواژه‌ها [English] | ||
| electrolyte leakage, Moicture Content at harvesting, Poly amine, Proline, seedling growth, Water | ||
| مراجع | ||
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