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بررسی تنوع ژنتیکی اکوتیپ‌های آویشن شیرازی با استفاده از نشانگرهای مولکولیRAPD و ISSR | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 11، دوره 26، شماره 1 - شماره پیاپی 51، تیر 1397، صفحه 101-113 اصل مقاله (1.12 M) | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.116660 | ||
| نویسندگان | ||
| حسین بی باک* 1؛ کیان آقاعباسی2 | ||
| 1نویسنده مسئول مکاتبات، مربی بخش زیست‌شناسی، دانشکده علوم، دانشگاه جیرفت پست الکترونیک: Hbibak@ujiroft.ac.ir | ||
| 2دانشجوی دکترای رشته بیوتکنولوژی کشاورزی، دانشگاه گیلان | ||
| چکیده | ||
| شناخت تنوع ژنتیکی از فعالیتهای مهم در بهنژادی و مدیریت حفظ ذخائر ژنتیکی گیاهان بهشمار می‌آید. باتوجه به مشاهده گوناگونی صفات در بین آویشن شیرازی، در این تحقیق از نشانگر RAPD و ISSRبهمنظور بررسی تنوع ژنتیکی بین جمعیتی آویشن شیرازی در ایران استفاده شد. برگهای آویشن از 15 مناطق مختلف کشوری از استانهای کرمان، سیستان وبلوچستان، بوشهر، فارس و خوزستان جمعآوری شدند. استخراج DNA از برگ بهکمک روش CTAB با کمی تغییر انجام گردید. ده نشانگر RAPDو ده نشانگر ISSR که باندهای واضحتری در واکنش زنجیرهای پلیمراز تولید کرده بودند برای تجزیه‌وتحلیل استفاده شدند. با کمک نرمافزاز NTSYS-pc با استفاده از ضریب تشابه دایس و الگوریتم میانگین فاصله (UPGMA) دندروگرام مربوطه رسم شد. ضریب کوفنتیک محاسبه شد و پلات دوبعدی بر اساس تجزیه به مؤلفههای اصلی شکل گرفت. باتوجه به نتایج حاصل، دامنه اندازه باندهای تولید شده در آغازگرها بین 120 تا 3100 جفت باز متغیر بود. پانزده منطقه انتخاب شده در 3 گروه مجزا قرار گرفتند .خوشه ایجاد شده با شرایط جغرافیایی همخوانی داشت. آغازگرها در مجموع 207 نوار چندشکل با درصد چندشکلی 9/86 تولید کردند. نتایج حاصل از ضریب تشابه دایس نرمافزار NTYSIS حکایت از این مطلب داشت که تشابه ژنتیکی توده آویشن شیرازی بین8460/0–4390/0 متغیر است. کمترین تشابه بین تودههای جیرفت با ایرانشهر و بیشترین تشابه بین نمونههای اندیمشک و ایذه مشاهده شد. نتایج تجزیه به مؤلفههای اصلی و نتایج حاصل از تجزیه خوشه‌ای با یکدیگر مشابه بودند و ضریب کوفنتیک 6/74 درصد بهدست آمد. نتایج نشان داد که نشانگرهای RAPD وISSR برای بررسی تنوع ژنتیکی بین جمعیتی این توده مناسب هستند. | ||
| کلیدواژه‌ها | ||
| آویشن شیرازی؛ تنوع ژنتیکی؛ نشانگرمولکولی؛ تجزیه خوشه‌ای | ||
| عنوان مقاله [English] | ||
| Assessment of genetic diversity of Zataria multiflora ecotypes by RAPD and ISSR | ||
| نویسندگان [English] | ||
| H. Bibak1؛ K. Aghaabbasi2 | ||
| 1Corresponding author, M.Sc., Department of Biology, Faculty of Science, University of Jiroft, Jiroft, I.R. Iran. Email: hbibak@ujiroft.ac.ir | ||
| 2PhD student, Department of Agriculture Biotechnology, Guilan University, Guilan, I.R. Iran. | ||
| چکیده [English] | ||
| Assessment of genetic diversity and germplasm classification of plant species are important activities in plant improvement and management of genetic resources. Regarding observed variation on various traits of Zataria multiflora Bosis. random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to assess genetic diversity of the species in Iran. Leaves were collected on thyme plants of 15 different regions of Kerman, Sistan and Balochistan, Boushehr, Fars, and Khuzestan provinces. Leaf DNA extraction was carried out by cetyltrimethyl ammonium bromide (CTAB) method with small modifications. Ten RAPD markers and 10 ISSR markers with sharper bands in polymerase chain reaction (PCR) were used for data analysis. NTSYS-pc software and dice similarity coefficient, as well as Unweighted Pair Group Method with Arithmetic Averages (UPGMA) were used to draw the related dendrogram. Cophenetic correlation coefficient was calculated and a two-dimensional graph was created based on principle component analysis (PCA) results. Range of the bands produced by the primers varied from 120 to 3100bp. The 15 selected regions were classified into three separate groups. The established clusters were in a good harmony with geographical conditions. Entirely the markers produced 207 bands with polymorphism percentage of 86.9. The results of the dice similarity coefficient produced by NTYSIS suggested that genetic similarity of thyme varied between 0.4390–0.8460. The most genetic similarity was observed between Andimeshk and Eizeh samples and the least similarity was observed between Iranshahr and Jiroft samples. Results of principle components analysis and cluster analysis were similar. Cophenetic correlation coefficient was 74.60%. The results suggested that RAPD & ISSR markers are useful for investigating genetic variability of Zatraia multiflora germplasm. | ||
| کلیدواژه‌ها [English] | ||
| cluster analysis, Genetic diversity, molecular markers, Zataria multiflora | ||
| مراجع | ||
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