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باززایی گیاه در معرض انقراض لاله واژگون (Fritillaria raddeana) از طریق ریزنمونههای گلبرگ و برگ | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 9، دوره 26، شماره 1 - شماره پیاپی 51، تیر 1397، صفحه 71-83 اصل مقاله (724.87 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2018.116658 | ||
| نویسندگان | ||
| سلاله صلاحی صدر1؛ هدایت زکی زاده* 2؛ محمد رضا نقوی3؛ جمال العلی الفتی4 | ||
| 1دانشجوی دکترای مهندسی علوم باغبانی (گیاهان زینتی)، دانشکده علوم کشاورزی، پردیس دانشگاهی دانشگاه گیلان | ||
| 2نویسنده مسئول مکاتبات، استادیار گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت. پست الکترونیک:Zakizadeh@guilan.ac.ir | ||
| 3استاد، گروه زراعت و اصلاح نباتات، پردیس کشاورزی و منابع طبیعی دانشگاه تهران | ||
| 4استادیار گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت | ||
| چکیده | ||
| لاله واژگون (raddeana Fritillaria)، یکی از منحصر بهفردترین و زیباترین گیاهان خودرو و بومی ایران است. این جنس از خانواده سوسن بهشدت در معرض انقراض قرار دارد. گونه Fritillaria raddeana مقاوم به خشکی، مناطق سنگلاخی و شیبدار میباشد و بهدلیل مطالعات محدود در این گونه، انجام هر گونه پژوهش در این گیاه اهمیت بهسزایی دارد. با وجود احتمال وقوع جهش، باززایی موفقیتآمیز پیشنیاز کلیه مطالعاتی است که باید در گونههای رو به انقراض انجام شود. این پژوهش کالوسزایی و باززایی غیرمستقیم را از کشت اندامهای گلبرگ و برگ F. raddeana در محیط درون شیشهای مورد بررسی قرار داد. ریزنمونههای برگ پیش از گلدهی و ریزنمونههای گلبرگ در حالت غنچه (سبز تا زرد) برداشت شد و پس از ضدعفونی، در محیط کشت موراشیگ و اسکوگ همراه با غلظتهای مختلف از اکسین و سایتوکینین کشت شدند. ریزنمونهها برای القای کالوس ابتدا در تاریکی و دمای C°18 و بعد به روشنایی با شرایط دمایی قبل انتقال یافتند. پس از واکشتِ کالوسها در محیطهای باززایی، دما به C°20 افزایش یافت. اگرچه هر دو نوع ریزنمونه بهدلیل عدم آسیب به گیاهان مادری، درصد نسبتا پایین آلودگی داخلی، تعداد و اندازه مناسب، ریزنمونههای قابل قبولی بودند، با اینحال ریزنمونه گلبرگ دارای پاسخدهی بهتر و سریعتر به انواع ترکیبات تنظیمکننده رشد بود. بهترین محیط کشت کالوسزایی (66/82%) و باززایی غیرمستقیم (66/36%) از ریزنمونههای گلبرگ، محیط MS حاوی 5/0 میلیگرم در لیتر ایندول بوتیریک اسید همراه با 2 میلیگرم در لیتر تیدیازورون تشخیص دادهشد که نشاندهنده برتری این دو نوع تنظیمکننده در باززایی غیرمستقیم F. raddeana بود. | ||
| کلیدواژهها | ||
| باززایی غیرمستقیم؛ تنظیمکننده رشد؛ کالوسزایی؛ گونههای وحشی؛ گیاه بومی | ||
| عنوان مقاله [English] | ||
| Regeneration of an endangered Fritillaria (Fritillaria raddeana) species via petal and leaf explants | ||
| نویسندگان [English] | ||
| S. Salahi Sadr1؛ H. Zakizadeh2؛ M.R. Naghavi3؛ J.A. Olfati4 | ||
| 1Ph.D. Student, Department of Horticultural Sciences, University Campus 2, University of Guilan, Rasht, I.R. Iran. | ||
| 2Corresponding Author, Assist. Prof., Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R. Iran. Email: Zakizadeh@guilan.ac.ir | ||
| 3Prof., Dept. of Agronomy and Plant Breeding, Agricultural & Natural Resources College, University of Tehran, Karaj, I.R. Iran | ||
| 4Assoc. Prof., Department of Horticultural Sciences, Faculty of Agricultural Sciences, University of Guilan, Rasht, I.R. Iran | ||
| چکیده [English] | ||
| Fritillaria raddeana is one of the most fascinating wild flowers, belonging to Lilliaceae family, native to Iran. It is an important ornamental-medical bulbous plant, tolerant to arid conditions and stony slopes but facing extinction. There have been very few reports exist, so any studies on the species would be of great importance. Regardless of mutation occurrence, successful regeneration will be a prerequisite for further researches that must be done on the endangered plant species. This research presents an in vitro callus induction and indirect regeneration of F. raddeana via culturing petal and leaf. The leaf (before flowering) and petal (green to yellow flower buds) explants excited from plant and surface sterilized and were cultured on MS media containing different concentrations of Auxin and Cytokinin. The explants were first kept at darkness and 18ºC for callus induction, then were transferred to light condition with the same temperature. The temperature was raised to 20ºC, when the calli sub cultured into regeneration media. Although due to the low amount of bacterial and fungal contamination, conservation of parent plants, adequate and ready available explants, both petals and leaves are acceptable explants, among them petal showed better and quicker response to variable plant growth regulators. The best medium for callus formation (82.66%) and indirect regeneration (36.66%) via petal explants was MS medium supplemented with 2 mgl-1 TDZ and 0.5 mgl-1 IBA. Therefore, the mentioned plant growth regulators were more suitable than the others for indirect regeneration of F. raddeana. | ||
| کلیدواژهها [English] | ||
| Callus formation, endemic plant, indirect regeneration, plant growth regulators, wild species | ||
| مراجع | ||
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