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Prevalence of Canine Monocytic Ehrlichiosis in Canine Population Across India | ||
| Archives of Razi Institute | ||
| مقاله 2، دوره 73، شماره 2، شهریور 2018، صفحه 87-93 اصل مقاله (271.35 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2018.116616 | ||
| نویسندگان | ||
| K. Kukreti1؛ L. Pandey* 2؛ M. Das3؛ A. Rastogi3؛ R. Dubey3؛ P. Sharma3 | ||
| 1Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh | ||
| 2St. aloysius College, Jabalpur (MP), India | ||
| 3Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, 250001, India | ||
| چکیده | ||
| Canine ehrlichiosis is a very important emerging disease in India. This study is the first attempt screening a large number of canines in India for the detection of canine monocytic ehrlichiosis. In the present study, 510 blood samples of dogs were screened for the presence of Ehrlichia canis and other variants of Anaplasmataceae family by serological and molecular methods.Out of the 510 serum samples, 293 (57.5%) cases were found positive for the presence of E. canis antibodies through enzyme-linked immunosorbent assay (ELISA). Furthermore, and 45 (8.8%) and 1 (0.2%) specimens were positive for E. canis and A. platys, respectively, based on the polymerase chain reaction (PCR). In the clinical samples of E. canis, the minimum detection limit for PCR was9 ng. In the immunofluorescence assay (IFA), the positive blood samples showed comparable results with those obtained from the commercially available dot ELISA kit (giving equivalent IFA titer). The results of sequencing were compared with other reported isolates in various regions of the world, and a phylogenetic relationship was established. The 16S rRNA region that was amplified and sequenced for E. canis and A. platys was highly conserved and so was another Vir B9 region. | ||
| کلیدواژهها | ||
| Ehrlichiosis canis؛ Anaplasma platys؛ Polymerase Chain Reaction؛ ELISA؛ India | ||
| عنوان مقاله [English] | ||
| مطالعه شیوع عفونت مونوسیتی سگان ارلی شیوز در جمعیتهای سگان در هند | ||
| نویسندگان [English] | ||
| Kartikaye Kukreti1؛ Laxmi Pandey2؛ Manoj Das3؛ Amit Rastogi3؛ Rahul Dubey3؛ Prashant Sharma3 | ||
| 1Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh | ||
| 2St. aloysius College, Jabalpur (MP), India | ||
| 3Biotechnology Section, Central Military Veterinary Laboratory, remount Veterinary Corps, Meerut Cantt, Uttar Pradesh, 250001, India | ||
| چکیده [English] | ||
| به دلیل خارجی بودن نویسنده مقاله فاقد چکیده فارسی است. | ||
| کلیدواژهها [English] | ||
| به دلیل خارجی بودن نویسنده مقاله فاقد کلید واژه فارسی است | ||
| مراجع | ||
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