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بافت‌شناختی تنش اکسیداتیو و تولید گونه‌های فعال اکسیژن در مقابل پیشرفت عامل بیماری آتشک در ارقام گلابی | ||
| به زراعی نهال و بذر | ||
| مقاله 1، دوره 33، شماره 2، بهمن 1396، صفحه 139-162 اصل مقاله (813.76 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/sppj.2018.116423 | ||
| نویسندگان | ||
| حمید عبدالهی* ؛ زینب صالحی | ||
| پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| مقاومت به بیماری آتشک در ارقام گلابی به دو صورت پیشرفت کند بیماری در ارقام نیمه‌مقاوم و توقف سریع بیماری در ارقام مقاوم بروز می‌کند. با توجه به نقش موثر و دوگانه گونه‌های فعال اکسیژن (ROS)، به ویژه سه گونه سوپراکسید (O2•-)، پراکسیدهیدروژن (H2O2) و هیدروکسیل (OH•-) در ایجاد بیماری و دفاع علیه آن، در این تحقیق تولید این گونه‌های فعال اکسیژن در رقم مقاوم درگزی، رقم نیمه مقاوم هاروسوئیت (Harrow Sweet) و رقم حساس ویلیامز (Williams) در واکنش به حمله باکتری Erwinia amylovora در شرایط درون‌شیشه‌ای مقایسه شد. سرعت پیشرفت نکروز در شاخه‌چه‌های درون شیشه بر اساس میزان حساسیت رقم در شرایط گلخانه‌ای بود. در هر سه گونه فعال اکسیژن، توسعه بافتی از استوانه مرکزی به سرعت به صورت عمودی آغاز و به تدریج به صورت افقی در پارانشیم ساقه توسعه یافت. دو شاخص شدت و سرعت تولید گونه‌های فعال اکسیژن در پی حمله عامل بیماری منطبق بودند، ولی در رابطه با رادیکال سوپراکسید، ارقام تفاوت قابل توجهی نشان ندادند. با توجه به نقش بازدارنده پراکسید هیدروژن در برابر عامل بیماری، در دو رقم هاروسوئیت و ویلیامز، الگوی تولید و گسترش آن توجیه کننده میزان مقاومت در ارقام بود. همچنین در رقم مقاوم درگزی، بیش‌ترین تاخیر در تولید پراکسید هیدروژن تا 96 ساعت پس از مایه‌زنی مشاهده شد. بر اساس الگوی مشاهده شده تولید گونه‌های فعال اکسیژن، به نظر می‌رسد ارقام مختلف گلابی از مکانیسم‌های متفاوتی برای مقابله با عامل بیماری آتشک استفاده می‌کنند. | ||
| کلیدواژه‌ها | ||
| ارقام گلابی؛ Erwinia amylovora؛ نکروز؛ هیدروکسیل؛ پراکسید هیدروژن؛ سوپراکسید | ||
| عنوان مقاله [English] | ||
| Histology of Oxidative Stress and Generation of Reactive Oxygen Species Against Progress of Fire Blight Causal Agent in Pear Cultivars | ||
| نویسندگان [English] | ||
| H. Abdollahi؛ Z. Salehi | ||
| Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده [English] | ||
| Resistance to fire blight disease in pears appears as slow disease progress in moderately resistant and sharp prevention of disease progress in resistant cultivars. Regarding to the importance and dual roles of reactive oxygen species (ROS), especially superoxide (O2•-), hydrogen peroxide (H2O2) and hydroxyl (OH•-) in disease development and defense against its progress, in this research, generation of these species of active oxygen were studied in pear cultivars Dar Gazi (resistant), Harrow Sweet (moderately resistant) and Williams (susceptible) after inoculation with Erwinia amylovora in in vitro conditions. The necrosis progress rates in in vitro shootlets were according to the disease susceptibility of cultivars and were comparable with the greenhouse results. For all species of active oxygen, first traces was observed in central cylinder of the in vitro shootlets in vertical direction, followed by horizontal expansion of active oxygen species in stem parenchyma. Two indices of velocity and severity of each singular active oxygen species were correspondence. Comparison of superoxide generation showed similar rates in all tested cultivars, while based on the deterrent role of H2O2 on disease progress, H2O2 generation in cultivar Harrow Sweet was earlier and higher than other cultivars. In pear cultivar Dar Gazi, H2O2 generation was postponed up to 96h after inoculation. According to the results, it seems that pear cultivars use different strategies for defense against fire blight invasion. | ||
| کلیدواژه‌ها [English] | ||
| Pear cultivars, Erwinia amylovora, necrosis, hydroxyl, hydrogen peroxide, superoxide | ||
| مراجع | ||
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Abdollahi, H., 2010. Pear, Botany, Cultivars and Rootstocks. Iranian Agricultural Ministry Publications. Tehran, Iran, 210pp. (in Persian).
Abdollahi, H., Ghahremani, Z., Erfani Nia, K., and Mehrabi, R. 2015. Role of electron transport chain of chloroplasts in oxidative burst of interaction between Erwinia amylovora and host cells. Photosynthesis Research 124: 231–242.
Abdollahi, H., Ghasemi, A., and Mehrabipour, S. 2008. Evaluation of fire blight resistance in some quince (Cydonia oblonga Mill.) genotypes, II. Resistance of genotypes to the disease. Seed and Plant 24: 529-541 (in Persian).
Abdollahi, H., and Majidi Heravan, E. 2005. Relation between fire blight resistance and different vegetative and reproductive traits in apple (Malus domestica Borkh.) cultivars. Seed and Plant 21: 501-513 (in Persian).
Abdollahi, H., Muleo, R., and Rugini, E. 2005. Study of basal growth media, growth regulators and pectin effects on micropropagation of pear (Pyrus communis L.) cultivars. Seed and Plant 21: 373-384 (in Persian).
Abdollahi, H., Ruzzi, M., Rugini, E., and Muleo, R. 2004. In vitro system for studying the interaction between Erwinia amylovora and genotypes of pear. Plant Cell, Tissue and Organ Culture 79: 203-212.
Ahmadi, S., Alipour, M., Abdollahi, H., and Atashkar, D. 2013. Comparison of efficiency of indices for fire blight susceptibility evaluation in quince (Cydonia oblonga Mill.) in orchard condition. Seed and Plant Improvement Journal 29-1: 331-347 (in Persian).
Aldwinckle, H. S., and Preczewski, J. L. 1976. Reaction of terminal shoots of apple cultivars to invasion by Erwinia amylovora. Phytopathology 66: 1439–1444.
Ali, B., and Kazempour, M. N. 2004. Presentation of Erwinia amylovora from Guilan province. Proceedings of the 16th Iranian Plant Protection Congress, Tabriz, Iran. Page 422 (in Persian).
Azarabadi, S. R. 2014. Assessment of resistance and survey on some tolerance mechanisms to fire blight (Erwinia amylovora) in some new pear cultivars and rootstocks. MSc. Thesis, College of Agriculture, Varamin- Pishva Branch, Islamic Azad University, Varamin, Iran. 153pp. (in Persian).
Azarabadi, S., Abdollahi, H., Torabi, M., Salehi, Z., and Nasiri, J. 2017. ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in response to Erwinia amylovora in pear (Pyrus communis L). European Journal of Plant Pathology 147: 279-294.
Bhattacharjee, S. 2011. Sites of generation and physicochemical basis of formation of reactive oxygen species in plant cell. pp. 1-30. In: Dutta Gupta, S. (ed.). Reactive Oxygen Species and Antioxidants in Higher Plants. CRC Press, Boca Raton, FL, USA.
Davoudi, A. 1998. Evaluation of fire blight resistance in some apple and pear cultivars. MSc. Thesis, University of Tabriz, Tabriz, Iran. 200pp. (in Persian).
Davoudi, A., Majidi Heravan, E., Rahimian, H., and Valizade, M. 2005. The intensity of disease of pear cultivars to fire blight with use the standard system of USDA. Journal of Science and Technology of Agriculture and Natural Resources, Water and Soil Science 9: 159-168 (in Persian).
Erfani, J., Abdollahi, H., Ebadi, A., Fatahi Moghadam, M. R., and Arzani, K. 2013. Evaluation of fire blight resistance and the related markers in some European and Asian pear cultivars. Seed and Plant Improvement Journal 29-1: 659-672 (in Persian).
Erfani, J., Ebadi, A., Abdollahi, H., and Fatahi Moghadam, M. R. 2012. Genetic diversity of some pear cultivars and genotypes using simple sequence repeat (SSR) markers. Plant Molecular Biology Reporter 30: 1065–1072.
Erfani, J., Ebadi, A., Abdollahi, H., and Fatahi Moghadam, M.R. 2014. Investigation of changes in antioxidant enzyme and total phenol level in some pear cultivars inoculated with fire blight disease. Iranian Journal of Horticultural Science 45: 127-136 (in Persian).
Gowda, S. S., and Goodman, R. N. 1970. Movement and persistence of Erwinia amylovora in shoot, stem and root of apple. Plant Disease Reporter 54: 576–580.
Halliwell, B., and Gutteridge, J. M. C. 1999. The Chemistry of Free Radicals and Related Reactive Species. Oxford University Press, Oxford, UK. 704pp.
Klein, S. M., Cohen, G., and Cederbaum, A. L. 1981. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating systems. Biochemistry 20: 6006-6012.
Leblay, C., Chevreau, E., and Robin, L.M. 1991. Adventitious shoot regeneration from in vitro leaves of several pear cultivar (Pyrus communis L.). Plant Cell, Tissue and Organ Culture 25: 99-105.
Le Deunff, E., Davoine, C., Le Dantec, C., Billard, J. P., and Huault, C. 2004. Oxidative burst and expression of germin/oxo genes during wounding of ryegrass leaf blades: comparison with senescence of leaf sheaths. Plant Journal 38: 421-431.
Maleki Balajoo, O., Keshavarzi, M., Rezaee-Danesh, Y., Damyar, S., and Jaafari, M. 2011. Fire blight resistance in a number of local Iranian apple germplasm. Seed and Plant Improvement Journal 27-1: 25-36 (in Persian).
Maroofi, A., and Mostafavi, M. 1996. Evaluation of the resistance of apple, pear and quince varieties to fire blight. Acta Horticulturae 411: 395-400.
Mazarei, M., Zakeri, Z., and Hassanzadeh, N. 1994. Fire blight situation on fruit trees in West Azerbaijan and Ghazvin provinces. Iranian Journal of Plant Pathology 30: 25-32 (in Persian).
Mehrabipour, S., Abdollahi, H., and Ghasemi, A. A. 2010. Study of rootstock role on susceptibility of some quince genotypes to fire blight disease. Applied Entomology and Phytopathology 78: 26-42. (in Persian).
Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
Nourmohammadi, N., Abdollahi, H., Moeini, A., and Roohalamin, E. 2015. Effects of growth media and Fe source on micropropagation and rooting of semi-dwarf pear rootstocks, Pyrodwarf and OH×F87. Seed and Plant Production Journal 31-2: 265-278 (in Persian).
Oh, C. S., and Beer, S. V. 2005. Molecular genetics of Erwinia amylovora involved in the development of fire blight. FEMS Microbiology Letters 253:185-192.
Oh, C. S., Carpenter, S. C. D., Hayes, M. L., and Beer, S. V. 2010. Secretion and translocation signals and DspB/F-binding domains in the type III effector DspA/E of Erwinia amylovora. Microbiology 156: 1211–1220.
Quoirin, M., and Lepoivre, P. 1977. Etude de mileux adaptes aux cultures in vitro de Prunus. Acta Horticulturae 78: 437-442.
Sahandpour, A., and Ghasemi, A. 2004. Occurrence of the fire blight of pome trees in Fars province. Proceedings of the 16th Iranian Plant Protection Congress, Tabriz, Iran, Page 429 (in Persian).
Steiner, P. W. 2000. Integrated orchard and nursery management for the control of fire blight. pp. 339-358.In: Vanneste, J. L. (ed.). Fire Blight: the Disease and its Causative Agent, Erwinia amylovora. CAB International Press, Wallingford, UK.
Tahzibi Hagh, F., and Abdollahi, H. 2009. Histological and pomological characterization of sclereid cell clusters in some Iranian local and European pear cultivars. Acta Horticulturae 877: 765-769.
Thordal-Christensen, H., Zhang, Z., Wie, Y., and Collinge D. B. 1997. Subcellular localization of H2O2 in plants, accumulation in papillae and hypersensitive response during the barley- powdery mildew interaction. Plant Journal 11: 1187-94.
van Der Zwet, T., and Keil, H. L. 1979. Fire Blight: A Bacterial Disease of Rosaceous Plants. United States Department of Agriculture, Agricultural Handbook No. 510, Washington D.C., USA. 650pp.
Venisse, J. S., Guller G., and Brisset M. N. 2001. Evidence for the involvement of an oxidative stress in the initiation of infection of pear by Erwinia amylovora. Plant Physiology 125: 2164-2172.
Xie, Z., and Chen, Z. 2000. Harpin-induced hypersensitive cell death is associated with altered mitochondrial functions in tobacco cells. Molecular Plant Microbe Interaction 13:183-190.
Zakeri, Z., and Sharif Nabi, B. 1991. Fire blight disease in Karaj. Proceedings of the 10th Iranian Plant Protection Congress, Kerman, Iran. page 157 (in Persian).
Zohur, A., and Rahmani Moghadam, N. 2004. Outbreak of fire blight in Khorasan province. Proceedings of the 16th Iranian Plant Protection Congress, Tabriz, Iran. Page 423 (in Persian).
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