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Bioinformatics Analysis of Upstream Region and Protein Structure of Fungal Phytase Gene | ||
| Archives of Razi Institute | ||
| مقاله 8، دوره 73، شماره 3، آذر 2018، صفحه 229-237 اصل مقاله (419.62 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.109655.1115 | ||
| نویسندگان | ||
| M. Gholizadeh* 1؛ M.R. Nassiry2؛ M. R. Saberi3؛ A. A. Haddad-Mashadrizeh4 | ||
| 1Department of Animal Sciences, Faculty of Animal and Food Sciences, Khuzestan Ramin Agriculture and Natural Resources University, Mollasani, Ahvaz, Iran | ||
| 2Department of Genetics, Faculty of Animal Sciences, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| 3Department of Medical Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 4Cell and Molecular Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده | ||
| Phytase increases the bioavailability of phytate phosphorus in seed-based animal feeds and reduces the phosphorus pollution of animal waste. Since most animal feeds for pellets are heated up to 65-80 °C, the production of a thermostable structure for phytase can be useful. In this study, we sought to perform bioinformatics analysis of the upstream region and protein structure of fungal phytase to improve its expression and thermostability properties. We used bioinformatics methods such as similarity search, multiple alignment, statistical analysis of physicochemical properties of amino acids, pattern recognition, and protein modeling to find out the effective factors in heat resistance of phytase. Change in Gibbs free energy (ΔG) of the best pattern promoter resulting from the interaction between RNA polymerase and the promoter sequences of modified genes of phytase was equal to -9 kcalmol-1, which is lower compared to other interactions. The evaluation of the three-dimensional structure of new phytases showed that amino acid substitutions aimed at improving thermostability did not change the form and structure of the protein. The results of Prochek, Whatcheck, and ERRAT for structural analysis and verification were 84, 72, and 70, respectively, that were satisfactory. | ||
| کلیدواژه‌ها | ||
| Fungal Phytase؛ Bioinformatics؛ Homology Modelling؛ Molecular Docking؛ Upstream Region Analysis | ||
| عنوان مقاله [English] | ||
| تجزیه و تحلیل بیوانفورماتیکی نواحی بالادست ژن و ساختار پروتئین فیتاز قارچی | ||
| نویسندگان [English] | ||
| مریم قلی‌زاده1؛ محمدرضا نصیری2؛ محمدرضا صابری3؛ علی‌اکبر حداد مشهدریزه4 | ||
| 1گروه علوم دامی، دانشکده علوم غذایی و جانوری، دانشگاه منابع طبیعی و کشاورزی رامین خوزستان، اهواز، ایران | ||
| 2گروه ژنتیک، دانشکده علوم دامی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| 3گروه شیمی پزشکی، دانشکده داروسازی، دانشگاه علوم پزشکی مشهد، مشهد، ایران | ||
| 4گروه تحقیقاتی بیوتکنولوژی سلولی و مولکولی، موسسه بیوتکنولوژی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| چکیده [English] | ||
| فیتاز آنزیمی است که به طور اختصاصی بر روی فیتات عمل می‌کند و با شکستن آن فسفر را آزاد و به فرم دردسترس برای حیوان در می‌آورد. یک راه موثر برای افزایش دسترسی به فسفر در خوراک حیوانات که مبتنی بر دانه است استفاده از فیتاز به عنوان مکمل می‌باشد. از آنجا که اکثرا خوراک حیوانات برای پلت شدن تا دمای 65-85 درجه سانتیگراد حرارت می‌بینند بنابراین تولید یک ساختار مقاوم به حرارت برای فیتاز می تواند مفید باشد. هدف از این مطالعه تجزیه و تحلیل بیوانفورماتیکی ناحیه بالادست و ساختار پروتئینی ژن فیتاز قارچی به منظور بهبود ویژگی‌های بیان و مقاومت گرمایی است. روش‌های بیوانفورماتیکی مانند جستجوی شباهت، هم‌ترازی چندگانه، تجزیه و تحلیل آماری خواص فیزیکوشیمیایی اسیدهای آمینه، شناسایی الگو و مدل سازی پروتئین برای پیدا کردن عوامل موثر بر مقاومت در برابر حرارت فیتاز بود. ما همچنین برهمکنش RNA پلی‌مراز با توالی راه‌انداز ژن اصلاح شده فیتاز را برای رسیدن به بهترین الگوی راه‌انداز که قادر به بیان پروتئین فیتاز مقاوم به حرارت باشد را مورد مطالعه قرار دادیم. ارزیابی ساختار سه بعدی فیتاز جدید نشان داد که جایگزینی اسید آمینه به منظور بهبود مقاوت حرارتی، شکل و ساختار پروتئین را تغییر نداده است. | ||
| کلیدواژه‌ها [English] | ||
| فیتاز قارچی, بیوانفورماتیک, مدل سازی همسانی, چفت مولکولی, تجزیه و تحلیل نواحی بالادست | ||
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