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تشکیل کالوس و باززایی علف دریایی از طریق کشت بافت | ||
| مجله علمی شیلات ایران | ||
| مقاله 1، دوره 9، شماره 3، مهر و آبان 1379، صفحه 1-10 اصل مقاله (295.79 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/isfj.2000.115978 | ||
| نویسندگان | ||
| حسن ابراهیم زاده؛ فرح کریمی؛ سید رضا ابهری | ||
| چکیده | ||
| کشت بافت ، کالوس | ||
| کلیدواژه‌ها | ||
| کشت بافت؛ کالوس | ||
| عنوان مقاله [English] | ||
| Callous Formation and Regeneration of Sargasopsis zanardinii through Culturing Media | ||
| نویسندگان [English] | ||
| H. Ebrahimzadeh؛ F. Karimi؛ R. Abhari | ||
| چکیده [English] | ||
| Noedays, seaweeds have many uses in food industry, weaving, pharmacy, medicine, biotechnology and so on. Many efforts has been made to regenerate seaweeds in artificial media in order to carry out genetic engineering process since 1970. In this study we have tried to establish a proper method for sterilization and tissue culture of brown seaweed, Sargasopsis zanardinii, in 1/2PES, PES and 2PES media. After sterilization, the explants of the seaweed were cultured in liquid and solid media. consequently turgidity in margin of explants, longitudinal cell divission and formation of hairy strands, and microcallous formation were showed by explants which cultured in PES and 2PES solid media. After transporting from liquid to solid media, juglike bodies were formed on the surface of solid PES media and then some brown strands were grown from these bodies that finally were flattened. None of these results was obtained from 1/2PES media. | ||
| کلیدواژه‌ها [English] | ||
| Tissue culture, Callous, Sargasopsis zanardinii | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 160 تعداد دریافت فایل اصل مقاله: 138 |
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