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استخراج و شناسایی ترکیب های شیمیایی موجود در عصاره استونی پوست سرخدار (Taxus baccata L.) | ||
| تحقیقات علوم چوب و کاغذ ایران | ||
| مقاله 9، دوره 33، شماره 1 - شماره پیاپی 62، فروردین 1397، صفحه 88-99 اصل مقاله (599.49 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijwpr.2017.115389.1436 | ||
| نویسندگان | ||
| صدیقه افراز1؛ سید خلیل حسینی هاشمی* 2 | ||
| 1کارشناسی ارشد، گروه علوم و صنایع چوب و کاغذ، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران | ||
| 2دانشیار، گروه علوم و صنایع چوب و کاغذ، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران | ||
| چکیده | ||
| در این بررسی عصاره پوست تنه درخت سرخدار (Taxus baccata L.) رویش یافته در ذخیره گاه جنگل افرا تخته علی آباد کتول واقع در استان گلستان به کمک حلال استون و به روش سوکسله استخراج گردید و سپس ترکیب های شیمیایی موجود در آن مورد شناسایی قرار گرفت. پوست دیسک های تهیه شده جداسازی و به آرد تبدیل شد. سپس عصاره محلول در حلال استون بر طبق روش استاندارد TAPPI تهیه گردید. به منظور سایلیل دار کردن ترکیب ها، مقداری از پودر خشک عصاره در یک ویال شیشه ای ریخته شد و مواد مشتق ساز (پیریدین، BSTFA و TMCS 1%) به آن اضافه شد و نمونه برای شناسایی به دستگاه کروماتوگرافی گازی- طیف سنجی جرمی (GC/MS) تزریق گردید. نتایج حاصل از این تحقیق نشان داد که میانگین درصد عصاره استونی پوست سرخدار 63/13 درصد بود. به طور کلی 46 ترکیب در پوست سرخدار مورد شناسایی قرار گرفت. عمده ترین ترکیب های مورد شناسایی شامل: تترا سیلوکسان، دکا متیل- (80/14%)، هگزا دکانوییک اسید، هیدروکسی استر (24/11%)، 3-(3- آمینو پروپیل)-6-(3،3- دی متیل آلیل آمینو) پیورین (85/9%)، دی- ریبوفورانوز، 1، 2، 3، 5- تتراکیس-O-(هیدروکسی)- (31/9%) و 2- متیل پایرولو]1، 2-a[ پایرازین-1(H2)-ان (73/6%) بودند. ترکیب های شیمیایی متفاوتی از جمله فنولی، فلاونویید، آلکالویید، اسید چرب، استر اسید چرب، قند، آلکان، الکل چرب، قند الکلی و استرویید در عصاره استونی پوست سرخدار یافته شد که بعضی از آنها را می توان به عنوان مواد حفاظتی برای از بین بردن موجودات مخرب چوب سنتز کرد. مهمترین ترکیب های سیتوتوکسیک و ضد سرطان شناسایی شده در عصاره استونی پوست سرخدار، 4- پیپریدینون، 2، 2، 6، 6- تترا متیل (19/0%) و فنول، 2، 4- بیس(1، 1- دی متیل اتیل)- (34/3%) بود. | ||
| کلیدواژه‌ها | ||
| پوست سرخدار؛ عصاره استونی؛ ترکیب های سیتوتوکسیک و ضد سرطان | ||
| عنوان مقاله [English] | ||
| Extraction and Identification of Chemical Composition within Acetone Extract of Taxus baccata L. Bark | ||
| نویسندگان [English] | ||
| Seddigheh Afraz1؛ Seyyed Khalil Hosseinihashemi2 | ||
| 1M.Sc., Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| 2Associate Prof., Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| چکیده [English] | ||
| In this research, the bark extract of the yew (Taxus baccata L.) tree trunk grow in AfraTakhte forest cache of Aliabad-e Katul) located in Golestan province was extracted with acetone solvent and by Soxhlet apparatus method and then the chemical components within it were identified. The bark of prepared disks was separated and converted to the flour. Afterwards, extract insoluble acetone solvent was prepared according to TAPPI standard method. In order to silylation of compounds, amount of dry powder of extract was poured into a glass vial and the derivative materials (pyridine, BSTFA + TMCS 1%) were added to it and to identification the sample was injected to the gas chromatography- mass spectrometry device. The results from this research indicated that the mean value of acetone extract of yew bark was 13.63%. Generally, 46 compounds were identified in the yew bark. The most abundant identified compounds were including: tetrasiloxane, decamethyl- (14.80%), hexadecanoic acid, hydroxy ester (11.24%), 3-(3-aminopropyl)-6-(3,3-dimethylallylamino)purine (9.85%), d-ribofuranose, 1,2,3,5-tetrakis-O-(hydroxy)- (9.31%), 2-methylpyrrolo[1,2-a]pyrazin-1(2H)-one (6.73%). The various chemical components such as phenolic, flavenoid, alkaloid, fatty acid, fatty acid ester, sugar, alkane, fatty alcohol, alcoholic sugar, and stroied were identified in the acetone extract of yew bark that some of them can synthesized as preservatives for inhibition of wood-degrading organisms. The most important identified cytotoxic and anticancer compounds in the acetone extract of yew bark has been 4-piperidinone, 2,2,6,6-tetramethyl- (0.19%) and phenol, 2,4-bis(1,1-dimethylethy)- (3.32%). | ||
| کلیدواژه‌ها [English] | ||
| Yew bark, acetone extract, cytotoxic and anticancer components | ||
| مراجع | ||
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