
Extraction and Determination of Tropan Alkaloids, Hyoscyamine and Scopolamine, from Different Parts of Hyoscyamus pusillus L. in Different Stages of Plant Growth | ||
تحقیقات گیاهان دارویی و معطر ایران | ||
Article 2, Volume 22, Issue 1 - Serial Number 31, April 2006, Pages 1-10 PDF (1.58 M) | ||
Document Type: Research Paper | ||
DOI: 10.22092/ijmapr.2006.114993 | ||
Authors | ||
K. Dialmaghani* 1; R. Kharvari-Nejad1; H. Fahimi1; H. Hekmat- shoar2 | ||
1Islamic Azad University, Science and Researches Branch | ||
2Tabriz University | ||
Abstract | ||
Hyoscyamus pusillus from Solanaceae family is a medicinal plant producing tropane alkaloids. For investigation of tropane alkaloids levels in H. pusillus, plant materials were collected during three different growth stages, vegetative stage, flowering stage and fruiting stage, from two regions of Azarbayjan, Marand and Tabriz. After extraction and purification of alkaloids from different parts of plants (root, stem, leaves, flowering tops and seeds), were assayed by HPLC. Furthermore, in two regions effects of environmental factors upon tropane alkaloid levels were investigated. The results showed that hyoscyamine and scopolamine content varied in three stages and in different parts of plants. The highest level of hyoscyamine in two regions was observed in leaves at flowering stage, whereas there was lowest level in stems at vegetative stage. Hyoscyamine was dominant alkaloid, with except of seeds, in all organs. Furthermore, the results showed that hyoscyamine and scopolamine content of plants in Marand region at all growth stages was higher than that of plants in Tabriz region. The results of investigation of effects environmental factors on alkaloid levels showed that some of this factors influenced production of tropane alkaloids. For example, as altitude up, alkaloid levels are raised. Furthermore, increased nitrogen and phosphorus concentrations in soil caused increasing alkaloid levels in plants. Whereas, in contrast, reduced potassium in soil caused increasing alkaloid levels. As plant grows alkaloid levels increase. This can be results from it when plant mature, high nitrogen enters hyoscyamine (and as a result scopolamine) structure. | ||
Keywords | ||
Hyoscyamus pusillus; tropane alkaloids; Hyoscyamine; Scopolamine | ||
References | ||
- امید بیگی، ر.، 1376. رهیافتهای تولید و فراوری گیاهان دارویی. جلد دوم. انتشارات طراحان نشر، تهران، 283 صفحه.
- خاتم ساز، م.، 1377 . فلور ایران، شماره 24، تیره سیب زمینی. انتشارات موسسه تحقیقات جنگلها و مراتع، تهران، 112صفحه.
- زرگری، ع.، 1368. گیاهان دارویی. جلد سوم. انتشارات دانشگاه تهران، تهران، 926صفحه.
- صمصام شریعت، س. ه.، 1371. عصاره گیری و استخراج مواد موثره گیاهان دارویی و روشهای شناسایی و ارزش یابی آنها. انتشارات مانی، اصفهان، 422صفحه.
- Ahmed , Z. F. and Fahmy, I. R., 1994. The effect of environment on the growth and alkaloidal content of Hyoscyamus muticus L., Journal of the American Pharmaceutical Association, 484-487. - Bashir Khan, M. and Harborne, J.B., 1991. Potassium deficiency increases tropane alkaloid synthesis in Atropa acuminata via argenine and ornithine decarboxylase levels, Phytochemistry, 30 (11): 3559-3563. - Bernath, J., 1986. Production ecology of secondary plants products. In: herb, spice and medicinal plants. Oryx press, Arizona, Vol. 1.562 p. - Chevallier, A. and Kindersley, D., 1996. The Encyclopedia of Medicinal Plants, 345 p. - Cordell, G. A., 1981. Introduction to alkaloids, John Willey and sons, New york , 567 p. - Doerk-Schmitz, K., Witte, L. and Alfermann, W., 1993. Tropane alkaloid patterns in plants and hairy roots of Hyoscyamus albus. Phytochemistry, 33 (4): 107-110. - Demeyer, K. and Dejaegere, R., 1992. Effect of the nitrogen form used in the growth medium (NO-3, NH+4) on alkaloid production in Datura stramonium L., Plant and Soil, 147: 79 – 86. - El Sheikh, M.O.A., El Hassan, G. M., Tayeb Abdel Hafeez, A.R., Abdalla, A.A. and Antoun, M.D., 1982. Studies on Sudanese medicinal plants III: indigenous Hyoscyamus muticus as commercial source hyoscyamine, Plant Medica, 45: 116-119. - Franz, C., 1983. Nutrient and water management for medicinal and aromatic plants. Acta Horticulture, 132: 203-215. - Hashimoto, T., Hagashi, A., Amono, Y., Kohono, J., Jwanari, H., Usada, S. and Yamada, Y., 1991. Hyoscyamine- 6hydroxylase, an enzyme involved in tropane alkaloid biosynthesis is localized at pericycle of root, The Journal of Biological Chemistry, 266(7): 4648-4653. - Kitamura, Y., Sato, M. and Miura, H., 1992. Differences of atropine esterase activity between intact roots and cultured roots of various tropane-alkaloid producing plants. Phytochemistry, 31(4):1191-1194. - Klan, Z.F., 1931. Influence of period of vegetation and development of plant on the alkaloidal content of Hyoscyamus niger L. American Pharmaceutical Association, XX (11): 1163- 1174. - Kutchan, T.M., 1995. Alkaloid biosynthesis- The basis for metabolic engineering of medicinal plants. The Plant Cell, 7: 1059-1070. - Maldoni, B., 1991. Alkaloids: isolation and purification. Journal of Chemical Education, 68(8): 700-703. - Oksman-Caldenty, K.M., Vrurela, H., Straub, A. and Hiltunen, R., 1987. Variation in the tropane alkaloid content of Hyoscyamus muticus plant and cell culture clones. Planta Medica, 53(4): 349-354. - Parr, A.J., Payne, J., Eagles, J., Chapman, B.T., Robins, R.J. and Rhodes, M.J.C., 1990. Variation in tropane alkaloid accumulation within the Solanaceae and strategies for its exploitation. Phytochemistry, 29(8): 2545-2550. - Sikuli, N.N. and Demeyer, K., 1997. Influence of the ion- composition of the medium on alkaloid production by "hairy roots" of Datura stramonium. Plant Cell, Tissue and Organ Culture, 47: 261-267. - Supria, K.B., 1998. Handbook of medicinal plants. Poiter publishers, India, 607 p. - Woo, S.H., Park, T.M. and Yang, T. W., 1997. Induction of branch roots by cutting method in Hyoscyamus niger root culture. Plant Cell, Tissue and Organ Culture, 48: 131-134. | ||
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