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Zhang, A.J., Liu, S.L., Zhu, J.Y., Gu, Z.M., Zhou, Z.M., Zhang, G.F., Lu, K.H.. (1395). Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers. سامانه مدیریت نشریات علمی, 15(4), 1425-1440. doi: 10.22092/ijfs.2018.114620
A.J. Zhang; S.L. Liu; J.Y. Zhu; Z.M. Gu; Z.M. Zhou; G.F. Zhang; K.H. Lu. "Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers". سامانه مدیریت نشریات علمی, 15, 4, 1395, 1425-1440. doi: 10.22092/ijfs.2018.114620
Zhang, A.J., Liu, S.L., Zhu, J.Y., Gu, Z.M., Zhou, Z.M., Zhang, G.F., Lu, K.H.. (1395). 'Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers', سامانه مدیریت نشریات علمی, 15(4), pp. 1425-1440. doi: 10.22092/ijfs.2018.114620
Zhang, A.J., Liu, S.L., Zhu, J.Y., Gu, Z.M., Zhou, Z.M., Zhang, G.F., Lu, K.H.. Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers. سامانه مدیریت نشریات علمی, 1395; 15(4): 1425-1440. doi: 10.22092/ijfs.2018.114620

Transcriptome analysis of the freshwater pearl mussel, Hyriopsis cumingii (Lea) using illumina paired-end sequencing to identify genes and markers

Iranian Journal of Fisheries Sciences
مقاله 12، دوره 15، شماره 4، دی 2016، صفحه 1425-1440    اصل مقاله (215.86 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114620
نویسندگان
A.J. Zhang؛ S.L. Liu؛ J.Y. Zhu؛ Z.M. Gu؛ Z.M. Zhou؛ G.F. Zhang؛ K.H. Lu*
چکیده
The transcriptome of triangle sail mussel Hyriopsis cumingii (Lea) using Illumina paired-end sequencing technology was conducted and analyzed. Equal quantities of total RNA isolated from six tissues, including gonad, hepatopancreas, foot, mantel, gill and adductor muscle, were pooled to construct a cDNA library. A total of 58.09 million clean reads with 98.48 % Q20 bases were generated. Clustering and assembling of these reads produced a non-redundant set of 92,347 unigenes with an average length of 1,150.61 bp. 11,174 unigenes were involved in the molecular function, cellular component and biological process categories by GO Gene Ontology）analysis. Potential genes and their functions were predicted by KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway mapping and COG（Cluster of Orthologous Groups of proteins）analysis. More than 8 putative genes of interest involved in sex determination/differentiation were identified. Furthermore, 8,014 SSRs and 38,288 SNPs were identified in this transcriptome dataset.
کلیدواژه‌ها
Hyriopsis cumingii؛ Illumina paired-end sequencing؛ Transcriptome؛ Sex determination؛ molecular marker
آمار
تعداد مشاهده مقاله: 138
تعداد دریافت فایل اصل مقاله: 146


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