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Identification of a sex-linked SCAR marker for Plecoglossus altivelis and its application for identifying gender in cultivated and wild populations | ||
| Iranian Journal of Fisheries Sciences | ||
| مقاله 10، دوره 13، شماره 4، دی 2014، صفحه 895-906 اصل مقاله (982.66 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114404 | ||
| نویسندگان | ||
| C Wang* ؛ M Li؛ J Wang؛ Z Tu؛ H Fan | ||
| چکیده | ||
| Ayu (Plecoglossus altivelis), one kinds of valuable cultured fish species, show almost no morphological difference between male and female until sexual maturity. Here, we report the identification of sex-linked markers for the ayu, based on Amplified Fragment Length Polymorphism (AFLP) generated from cultured fish (15 males and 15 females) by using 63 different primer combinations. Genomic fragments (n = 3733) were produced with a mean frequency of 59 bands per primer pair. A male-specific of 139 base pair band was amplified and converted to a sequence-characterized amplified region (SCAR marker) designated as Ayu102. Six distinct genomic fragments were produced in 12 wild samples (6 males and 6 females). The fragments designated a, b, c, d, and e were detected only in males, and one fragment (f) was detected in both genders. Nucleotide sequence analysis showed that fragments e and f were 96.67% identical. Ayu102 marker was detected in 45-d-old larvae, in both cultured and wild fish populations, and in offsprings generated by gynogenesis. Fragment e was detected in all males, and in only 6.7% of females in cultured fish. Data suggest that Ayu102 marker is a male-specific marker linked to the sex-determining locus of the male ayu and can be used for gender identification. | ||
| کلیدواژهها | ||
| AFLP؛ Plecoglossus altivelis؛ Sex-linked marker | ||
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