اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها2,873
تعداد مقالات32,555
تعداد مشاهده مقاله42,418,873
تعداد دریافت فایل اصل مقاله19,194,474
Mirbakhsh, M, Akhavan sepahy, A, Afsharnasab, M, Khanafari, A, Razavi, M.R. (1393). Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing. سامانه مدیریت نشریات علمی, 13(2), 384-393. doi: 10.22092/ijfs.2018.114364
M Mirbakhsh; A Akhavan sepahy; M Afsharnasab; A Khanafari; M.R Razavi. "Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing". سامانه مدیریت نشریات علمی, 13, 2, 1393, 384-393. doi: 10.22092/ijfs.2018.114364
Mirbakhsh, M, Akhavan sepahy, A, Afsharnasab, M, Khanafari, A, Razavi, M.R. (1393). 'Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing', سامانه مدیریت نشریات علمی, 13(2), pp. 384-393. doi: 10.22092/ijfs.2018.114364
Mirbakhsh, M, Akhavan sepahy, A, Afsharnasab, M, Khanafari, A, Razavi, M.R. Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing. سامانه مدیریت نشریات علمی, 1393; 13(2): 384-393. doi: 10.22092/ijfs.2018.114364

Molecular Identification of Vibrio harveyi From Larval Stage of Pacific White Shrimp (Litopenaeus vannamei) Boone (Crustacea:Decapoda)By Polymerase Chain Reaction and 16S rDNA Sequencing

Iranian Journal of Fisheries Sciences
مقاله 11، دوره 13، شماره 2، تیر 2014، صفحه 384-393
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114364
نویسندگان
M Mirbakhsh* ؛ A Akhavan sepahy؛ M Afsharnasab؛ A Khanafari؛ M.R Razavi
چکیده
A Gram-negative, rod-shaped, motile and halophilic bacterium identified as Vibrio harveyi was isolated from hatchery-reared larval of Iran Shrimp Research Centerfollowing mass mortality . Seven isolates of Vibrio spp.were collected from nauplii of Litopenaeus vannamei and then were evaluated for characteristics including morphology, physiology and biochemistry. The results indicated that the isolates belonged to a single species that grew on Thiosulfate citrate- Bile Sucrose agar and in 1.5 to 6% NaCl, positive lysine and ornithine decarboxylation and susceptible to O/129 disk. Identification of Vibrio harveyi strainIS01 (1 of 7 isolates) was confirmed by Polymerase Chain Reaction assay for Vibrio harveyi (expected amplicon 1493 bp). The 16S ribosomal DNA sequence (National Center for Biotechnology Information, GenBank accession number GU974342.1) gave 99% sequence identity to V. harveyi ATCC: 35084 (GenBank accession number EU130475.1).
کلیدواژه‌ها
Vibriosis؛ shrimp diseases؛ Litopenaeus vannamei؛ 16S rDNA sequencing
آمار
تعداد مشاهده مقاله: 155


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب