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Motamedi Sedeh, F., Afsharnasab, M., Heidarieh, M., Shafaee, S. K., Rajabifar, S., Dashtiannasab, A., Razavi, M. H.. (1390). Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. سامانه مدیریت نشریات علمی, 11(1), 145-155. doi: 10.22092/ijfs.2018.114191
F. Motamedi Sedeh; M. Afsharnasab; M. Heidarieh; S. K. Shafaee; S. Rajabifar; A. Dashtiannasab; M. H. Razavi. "Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus". سامانه مدیریت نشریات علمی, 11, 1, 1390, 145-155. doi: 10.22092/ijfs.2018.114191
Motamedi Sedeh, F., Afsharnasab, M., Heidarieh, M., Shafaee, S. K., Rajabifar, S., Dashtiannasab, A., Razavi, M. H.. (1390). 'Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus', سامانه مدیریت نشریات علمی, 11(1), pp. 145-155. doi: 10.22092/ijfs.2018.114191
Motamedi Sedeh, F., Afsharnasab, M., Heidarieh, M., Shafaee, S. K., Rajabifar, S., Dashtiannasab, A., Razavi, M. H.. Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus. سامانه مدیریت نشریات علمی, 1390; 11(1): 145-155. doi: 10.22092/ijfs.2018.114191

Titration of the Iranian White Spot Virus isolate, on Crayfish Astacus leptodactylus and Penaeus semisulcatus

Iranian Journal of Fisheries Sciences
مقاله 12، دوره 11، شماره 1، فروردین 2012، صفحه 145-155    اصل مقاله (483.11 K)
شناسه دیجیتال (DOI): 10.22092/ijfs.2018.114191
نویسندگان
F. Motamedi Sedeh* ؛ M. Afsharnasab؛ M. Heidarieh؛ S. K. Shafaee؛ S. Rajabifar؛ A. Dashtiannasab؛ M. H. Razavi
چکیده
  White Spot Virus (WSV) is currently the most serious viral pathogen of shrimp worldwide it causes mortality up to 100% within 7-10 days in commercial shrimp farms. Infected Indian white shrimp Fenneropenaeus indicus samples were collected from Guatr shrimp site in Sistan and Baluchestan province in south of Iran and WSV infection was confirmed by Nested PCR. WSV was isolated from infected shrimp samples by centrifugation and filtration and multiplied in crayfish by intramuscular inoculation, the isolated virus was called WSV/IRN/1/2010. In order to determine the dilution resulting in 90-100% mortality in Penaeus semiculcatus, diluted virus stock in steps from 100 till 105 times in sterile PBS was injected intramuscularly to 14 shrimps in each group. Also the virus stock was diluted in steps from 1/2 till 1/32 times in sterile PBS and injected intramuscularly in Astacus leptodactylus crayfish. Therefore the LD50 of live virus stock in Astacus leptodactylus and Penaeus semiculcatus crayfish were calculated by the Karber method 10 3.29 /ml and 10 5.35 /ml, respectively.
کلیدواژه‌ها
White Spot Virus؛ Titration؛ Karber Formula؛ ASTACUS LEPTODACTYLUS؛ PENAEUS SEMISULCATUS
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