| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 2,784 |
| تعداد مقالات | 31,679 |
| تعداد مشاهده مقاله | 40,110,194 |
| تعداد دریافت فایل اصل مقاله | 17,604,386 |
تولید فاژهای پلیکلونال حاوی قطعات ژنی آنتیبادی اختصاصی باکتری Xanthomonas citri subsp. citri با استفاده از فنآوری نمایش فاژی | ||
| آفات و بیماری‌های گیاهی | ||
| مقاله 11، دوره 85، شماره 2 - شماره پیاپی 105، اسفند 1396، صفحه 265-276 اصل مقاله (236.33 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2017.115980.1194 | ||
| نویسندگان | ||
| حمیده رئیسی1؛ محمد رضا صفرنژاد* 2؛ سید مهدی علوی3؛ سیدعلی الهی نیا4؛ ناصر فرخی5 | ||
| 1دانش آموخته دکتریt ﺑﺨﺶ گیاهپزشکی، دانشکده علوم کشاورزی، ﺩﺍﻧﺸﮕﺎﻩ گیلان، رشت | ||
| 2دانشیار پژوهش، ﺑﺨﺶ ﻭﻳﺮﻭﺱ ﺷﻨﺎﺳﻲ ﮔﻴﺎﻫﻲ، ﻣﻮﺳﺴﻪ ﺗﺤﻘﻴﻘﺎﺕ ﮔﻴﺎﻩ ﭘﺰﺷﻜﻲ ﻛﺸﻮﺭ، سازمان تحقیقات آموزش و ترویج کشاورزی، ﺗﻬﺮﺍﻥ | ||
| 3استادیار پژوهش، ﺑﺨﺶ ﺑﻴﻮﺗﻜﻨﻮﻟﻮژﻱ ﮔﻴﺎﻫﻲ، ﭘﮋﻭﻫﺸﮕﺎﻩ ﻣﻠﻲ ﻣﻬﻨﺪﺳﻲ ژﻧﺘﻴﻚ ﻭ ﺯﻳﺴﺖ ﻓﻦ ﺁﻭﺭﻱ، تهران | ||
| 4استاد، ﺑﺨﺶ گیاهپزشکی، دانشکده علوم کشاورزی، ﺩﺍﻧﺸﮕﺎﻩ گیلان، رشت | ||
| 5دانشیار، گروه بیولوژی سلولی و مولکولی، دانشکده علوم زیستی و بیوتکنولوژی، دانشگاه شهید بهشتی، تهران | ||
| چکیده | ||
| شانکر باکتریایی مرکبات از بیماری‌های باغات لیمو در جنوب کشور با عامل Xanthomonas citri subsp. citriمیباشد. تکنیک فاژنمایی از راهکارهای تولید آنتیبادیهای اختصاصی به منظور شناسایی گیاهان آلوده و همچنین تولید گیاهان مقاوم به بیماری است. در این تحقیق قابلیت استفاده از این سیستم برای تولید فاژهای نوترکیب حاوی قطعات آنتیبادی اختصاصی برعلیه باکتری عامل بیماری شانکر مرکبات بررسی شد. برای این منظور، پروتئین تاثیرکننده (pthA) و پیلوس (HrpE) که از اجزای اساسی سیستم ترشحی نوع سه باکتری هستند و نقش ضروری در بیماریزایی پاتوژن دارند، به عنوان آنتیژن انتخاب شدند. ابتدا پروتئین‌های pthA و HrpE به صورت نوترکیب در میزبان باکتریایی تولید شدند و با روش کروموتوگرافی خالص گردیدند. از کتابخانه های فاژی حاوی قطعات ژنی نواحی متغیر آنتیبادی برای جداسازی فاژهای اختصاصی استفاده شد. غنیسازی جمعیت فاژهای اختصاصی با انجام سه مرحله غربالگری بر علیه پروتئین های خالص شده pthA و HrpEانجام شد و اختصاصیت فاژهای حاصله بر علیه آنتیژن توسط آزمون الیزا بررسی شد. فاژهای نوترکیب قابلیت اتصال به پروتئین‌های pthA و HrpE را داشته و قادر به ردیابی گیاهان آلوده به بیماری شانکر باکتریایی مرکبات نیز بودند. از فاژهای جداسازی شده میتوان به منظور تولید آنتیبادی اختصاصی مونوکلونال استفاده نمود. | ||
| کلیدواژه‌ها | ||
| افکتور پروتئین pthA؛ پروتئین HrpE؛ شانکر باکتریایی مرکبات؛ غربالگری؛ نمایش فاژی | ||
| عنوان مقاله [English] | ||
| Production of polyclonal phages harbouring antibody fragment genes against Xanthomonas citri subsp. citri using phage display technology | ||
| نویسندگان [English] | ||
| H. RAEISI1؛ M. R. SAFARNEJAD2؛ S. M. ALAVI3؛ S. A. ELAHINIA4؛ N. FARROKHI5 | ||
| 1Plant protection,agriculture,guilan university | ||
| 2Department of Plant Viruses, Iranian Research Institute of Plant Protection, Agricultural research education and extension organization of Iran. Tehran | ||
| 3Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran | ||
| 4Department of Plant Protection, College of Agricultural Sciences, Guilan University, Rasht | ||
| 5Departement of Plant Sciences & Biotechnology, Faculty of Life Sciences & Biotechnology, Shahid Beheshti University G.C, Tehran | ||
| چکیده [English] | ||
| Citrus bacterial canker, caused by Xanthomonas citri subsp. citri (Xcc),is amongst the important diseases of lime orchards in southern parts of Iran. Phage display has been used to produce specific antibodies for detection of pathogen-infected plants as well as development of resistant varieties. An effector, namely pthA and a pilus protein, HrpE, the major critical components of type III secretion (T3S) system with roles in pathogenesis, were chosen as antigens. Recombinant forms of the proteins (pthA and HrpE) were expressed in a bacterial host and purified via affinity chromatography. Tomlinson phage display libraries including single chain variable fragments were used for isolation of the specific antibodies. Biopanning, 3 rounds against pthA and HrpE proteins, allowed enriching antigen-specific phages. The specificity of phages was tested using ELISA. Moreover, the phages were able to detect the plants infected with citrus bacterial canker. | ||
| کلیدواژه‌ها [English] | ||
| Biopanning, citrus bacterial canker, effector protein pthA, HrpE protein, phage display | ||
| مراجع | ||
|
ALIZADEH, A. and H. RAHIMIAN, 1990. Citrus canker in Kerman province. Iran Journal. Plant Pathology, 26: 42. (in persian with english summary)
AUSUBEL, F., R. BRENT, R. KINGSTONE, D. MOORE, J. G. SEIDMAN, J. A. SMITH and K. STRUHL, 1995. Current Protocols in Molecular Biology. 4648 Pp. New York, Wiley Interscience.
BARBAS, C. F., D. R. BURTON, J. K. SCOTT and G. J. SILVERMAN, 2001. Phage Display: A Laboratory Manual. CSH Laboratory Press, Cold Spring Harbor, NY.
BERRY, J. D. 2005. Rational monoclonal antibody development to emerging pathogens, biothreat agents and agents of foreign animal disease: The antigen scale. The Veterinary Journal, 170: 193–211.
CLACKSON, T., H. R. HOOGENBOOM, A. D. GRIFFITHS and G. WINTER, 1991. Making antibody fragments using phage display libraries. Nature, 352 (6336): 624-628.
COLETTA-FILHO, H. D., M. A. TAKITA, A. A. SOUZA, J. R. NETO, S. A. DESTEFANO, J. S. HARTUNG and M. A. MACHADO, 2006. Primers based on the rpf gene region provide improved detection of Xanthomonas axonopodis pv. citri in naturally and artificially infected citrus plants. Journal Application Microbiology, 100:279-285.
CORNELIS, G. R. and F. VAN GIJSEGEM, 2000. Assembly and function of type III secretory systems. Annual Review of Microbiology, 54: 735-774.
COOMBER, D. W. J. 2002. Panning of Antibody Phage-Display Libraries. In: O’Brien, P.M., R. Aitken, (eds) Antibody Phage Display. Methods in Molecular Biology, 178:133-45.
CUBERO, J. and J. H. GRAHAM, 2005. Quantitative real-time polymerase chain reaction for bacterial enumeration and allelic discrimination to differentiate xanthomonas strains on citrus. Phytopathology, 95:1333-1340.
DUAN, Y. P., A. L. CASTANEDA, G. ZHAO, G. ERDOS, and D. W. GABRIEL, 1999. Expression of a single, host-specific, bacterial pathogenicity gene in plant cells elicits division, enlargement and cell death. Molecular. Plant Microbe Interaction, 12: 556 – 560
EBRAHIMI, Y. 2009. Citrus situation in Iran. Ministry of Jihad e Agriculture. pP39, (In Farsi)
FANG, Y. and R. P. RAMASAMY, 2015. Current and prospective methods for plant disease detection. Biosensors, 4: 537–561.
FISCHER, R., N. EMANS and S. SCHILLBERG, 2001. Achieving plant disease resistance by antibody expression. Canadian Journal Plant Pathology, 23: 236–45.
FU, W., J. LIN and P. CEN, 2007. 5-Aminolevulinate production with recombinant Escherichia coli using a rare codon optimizer host strain. Applied Microbiology and Biotechnology, 75:777-782.
FUJIKAWA, T., H. ISHIHARA, J. E., LEACH and S. TSUYUMU, 2006. Suppression of defense response in plants by the avrBs3/PthA gene family of Xanthomonas spp. Molecular. Plant Microbe Interaction. 19: 342–349.
GOTTWALD, T. R., G. HUGHES, J. H. GRAHAM, X. SUN and T. RILEY, 2001. The citrus canker epidemic in Florida: the scientific basis of regulatory eradication policy for an invasive species. Phytopathology, 91:30-34.
GOTTWALD, T. R., J. H. GRAHAM and T. S. SCHUBERT, 2002. Citrus Canker: The pathogen and its impact. Plant Health Progress, DOI:10.1094/PHP-2002-0812-01-RV.
HOOGENBOOM, H. R. and P. CHAMES, 2000. Natural and designer binding sites made by phage display technology. Immunology Today, 21: 371–378.
HU, Z. Q., J. L. LIU, H. P. LI, S. XING, S. XUE, J. B. ZHANG, J. H. WANG, G. NoLKE and Y. C. LIAO, 2012. Generation of a highly reactive chicken-derived single-chain variable fragment against Fusarium verticillioides by phage display. International Journal of Molecular Sciences, 13(6): 7038–7056.
HUANG, J., B. RU and P. DAI, 2011. Bioinformatics resources and tools for phage display. Molecules, 16: 694–709.
JALAN, N., D. KUMAR, M. O. ANDRADE, F. YU, J. B. JONES, J. H. GRAHAM, F. F. WHITE, J. C. SETUBAL and N. WANG, 2013. Comparative genomic and transcriptome analyses of pathotypes of Xanthomonas citri subsp. citri provide insights into mechanisms of bacterial virulence and host range. BMC Genomics, 14:551.
KIERNY, R. M., T. D CUNNINGHAM and B. K. KAY, 2012. Detection of Biomarkers Using Recombinant Antibodies Coupled to Nanostructured Platforms. Nano Reviews, 3: 17240.
KOEBNIK, R. 2005. The type III-dependent Hrp pilus is required for productive interaction of Xanthomonas campestris pv.vesicatoria with pepper host plants. Journal of Bacteriology, 187: 24587
KREBBER, A., S. BORNHAUSER, J. BURMESTER, A. HONEGGER, J. WILLUDA, H. R. BOSSHARD, 1997. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. Journal of Immunological Methods, 201: 35-55.
LE GALL, F., J. M. BOVE and M. GARNIER, 1998. Engineering of a single-chain variable-fragment (scfv) antibody specific for the stolbur phytoplasma (mollicute) and its expression in Escherichia coli and tobacco plants. applied and environmental microbiology, 64: 4566–72.
MAVRODIEVA, V., L. LEVY and D. W. GABRIEL, 2004: Improved sampling methods for realtime polymerase chain reaction diagnosis of citrus canker from field samples. Phytopathology, 94:61-68.
MOKHTARI, M., M. R. SAFARNEJAD, S. M. ALAVI and A. TORKAMANZEHI, 2015. Isolation, gene expression and PthA effector protein production of Xanthomonas citri subsp citri causal agent of citrus bacterial canker. Journal of Agricultural Biotechnology, 7(2): 155-170. (In persian with english summary)
MOSTOFIZADEH-GHALAMFARSA, R. and H. RAHIMIAN, 1996. Incidence of the Asiatic form of citrus canker in Iran. Iranian Journal of Plant Pathology, 32: 189 (short report).
PRUVOST, O., B. BOHER, C. BROCHERIEUX, M. NICOLE and F. CHIROLEU, 2002. Survival of Xanthomonas axonopodis pv. citri in leaf lesions under tropical environmental conditions and simulated splash dispersal of inoculums. Phytopathology, 92: 336–346.
RAKONJAC, J., N. J. BENNETT, J. SPAGNUOLO, D. GAGIC and M. RUSSEL, 2011. Filamentous bacteriophage: biology, phage display and nanotechnology applications. Current Issues in Molecular Biology, 13: 51–57.
RIGANO, L. A., M. R. MARANO, A. P. CASTAGNARO, A. M. D. AMARAL and A. A. VOJNOV, 2010. Rapid and sensitive detection of citrus bacterial canker by loop-mediated isothermal amplification combined with simple visual evaluation methods. BMC Microbiology, 10: 176.
ROSANO, G. L. and E. A. CECCARELLI, 2014. Recombinant protein expression in Escherichia coli: advances and challenges. Frontiers in Microbiology, 5: 172.
SAFARNEJAD, M. R., G. R. SALEHI JOUZANI, M. TABATABAIE, R. M. TWYMAN and S. SCHILLBERG, 2011. Antibody-mediated resistance against plant pathogens. Biotechnology Advances, 29 (6): 961–971.
SCHAAD, N. W., E. POSTNIKOVA, G. LACY, A. SECHLER, I. AGARKOVA, P. E. STORMBERG, V. K. STORMBERG and A. K. VIDAVER, 2006. Emended classification of Xanthomonad pathogens on citrus. Systemaic and Applied Microbiology, 29: 690–695.
SCHIRRMANN, T., T. MEYER, M. SCHUTTE, A. FRENZEL and M. HUST, 2011. Phage display for the generation of antibodies for proteome research, diagnostics and therapy. Molecules, 16: 412-426.
SHAHRYARI, F., M. SHAMS-BAKHSH, M. R. SAFARNEJAD, N. SAFAIEE and S. ATAIE KACHOIEE, 2013a. Preparation of polyclonal antibody against Immunodominant membrane protein (IMP) of Candidatus Phytoplasma aurantifolia. Iranian Journal of Biotechnology, 11: 14-21.
SHAHRYARI, F., M. R. SAFARNEJAD, M. SHAMS-BAKHSH, G. NOLKE and S. SCHILLBERG, 2013b. Generation and expression on plants of a single-chain variable fragment antibody against the immunodominant membrane protein of Candidatus Phytoplasma aurantifolia. Journal of Microbiology and Biotechnology, 23(8): 1047-1054.
SHEETS, M. D., P. AMERSDORFER, R. FINNERN, P. SARGENT, E. LINDQUIST, R. SCHIER, G. HEMINGSEN, C. WONG, J. C. GERHART, J. D. MARKS and E. LINDQUIST, 1998. Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens. Proceedings of the National Acadamy of Science, 95: 6157-6162.
SWARUP, S., Y. YANG, M. T. KINGSLEY and D. W. GABRIEL, 1992. An Xanthomonas citri pathogenicity gene, PthA, pleiotropically encodes gratuitous avirulence on nonhosts. Molecular. Plant Microbe Interaction, 5: 204–213.
SUN, X., R. E. STALL, J. B. JONES, J. CUBERO, T. R. GOTTWALD, J. H. GRAHAM, W. N. DIXON, T. S. SCHUBERT, P. H. CHALOUX, V. K. STROMBERG, G. H. LACY and B. D. SUTTON, 2004. Detection and character ization of a new strain of citrus canker bacteria from Key/Mexican lime and alemow in South Florida. Plant Diseases, 88: 1179–1188.
TAVLADORAKI, P., E. BENVENUTO, S. TRINCA, D. DE MARTINIS, A. CATTANEO and P. GALEFFI, 1993. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature, 366: 469–72.
TEGEL, H., S. TOURLE, J. OTTOSSON and A. PERSSON, 2010. Increased levels of recombinant human proteins with the Escherichia coli strain Rosetta (DE3). Protein Expression and Purification, 69:159-167.
WEISSER, N. E. and J. C. HALL, 2009. Applications of single-chain variable fragment antibodies intherapeutics and diagnostics. Biotechnology Advances, 27: 502-20
VAUGHAN, T. J., A. J. WILLIAMS, K. PRITCHARD, J. K. OSBOURN, A. R. POPE, J. C. EARNSHAW, J. MCCAFFERTY, R. A. HODITS, J. WILTON and K. S. JOHNSON, 1996. Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature Biotechnology. 14: 309-314.
VERNIERE, C., J. S. HARTUNG, O. P. PRUVOST, E. L. CIVEROLO, A. M. ALVAREZ, P. MAESTRI and J. LUISETTI, 1998. Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from Southwest Asia. European Journal of Plant Pathology, 104: 477–487. | ||
|
آمار تعداد مشاهده مقاله: 674 تعداد دریافت فایل اصل مقاله: 452 |
||