Gharaei, A., porkazemi, M., Rezvani, S., Amiri majazi, B. (2005). Genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii by means of RAPD Technique. , 14(2), 91-102. doi: 10.22092/isfj.2005.113835
A. Gharaei; M. porkazemi; S. Rezvani; B. Amiri majazi. "Genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii by means of RAPD Technique". , 14, 2, 2005, 91-102. doi: 10.22092/isfj.2005.113835
Gharaei, A., porkazemi, M., Rezvani, S., Amiri majazi, B. (2005). 'Genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii by means of RAPD Technique', , 14(2), pp. 91-102. doi: 10.22092/isfj.2005.113835
Gharaei, A., porkazemi, M., Rezvani, S., Amiri majazi, B. Genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii by means of RAPD Technique. , 2005; 14(2): 91-102. doi: 10.22092/isfj.2005.113835

Genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii by means of RAPD Technique

مجله علمی شیلات ایران
Article 9, Volume 14, Issue 2, December 2005, Pages 91-102    PDF (466.1 K)
Document Type: Research Paper
DOI: 10.22092/isfj.2005.113835
Authors
A. Gharaei1; M. porkazemi2; S. Rezvani3; B. Amiri majazi4
1Zabol
2Rasht
3tehran
4Karaj
Abstract
We studied genetic difference and resemblance between Acipenser persicus and Acipenser gueldenstaedtii using Random Amplified Polymorphic DNA (RAPD) technique. The DNA of tail fin tissue of three A. persicus and A. gueldenstaedtii were extracted using phenol-chloroform method. After electrophoresis of the samples by agarose gel, their concentrations were regulated and Polymerase Chain Reaction (PCR) was conducted by 53 primers. PCR products were electrophoresed on polyacrylamide gel and silver staining was done to reveal the DNA bands of the samples.
 Among 53 primers, 17 had no site on genomic DNA of A. persicus and A. gueldenstaedtii and did not produce any bands while the remaining 36 primers showed band pattern. Analyzing the PCR products data using RAPD PLOT program showed that the maximum and minimum genetic distance between species were 73% and 65% respectively. Also, the mean difference between the species was 70% and the maximum and minimum genetic resemblance between the two species were 35% and 27% respectively. Based on the results, we conclude that A. persicus is an independent species from A. gueldenstaedtii.
Keywords
RAPD technique; Acipenser persicus; ACIPENSER GUELDENSTAEDTII; Caspian Sea; Iran
References
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