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تأثیر نانو اکسید روی و باکتریهای محرک رشد بر فعالیت آنزیم فسفاتاز اسیدی، میزان روی، پروتئین و صفات وابسته به رشد دانه تریتیکاله | ||
| علوم و فناوری بذر ایران | ||
| مقاله 13، دوره 6، شماره 1 - شماره پیاپی 11، خرداد 1396، صفحه 151-163 اصل مقاله (679.99 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2017.113549 | ||
| نویسندگان | ||
| حسین کمری1؛ رئوف سید شریفی* 2 | ||
| 1دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| 2عضو هیئت علمی/دانشکده کشاورزی، دانشگاه محقق اردبیلی، اردبیل، ایران. | ||
| چکیده | ||
| به منظور بررسی تأثیر نانو اکسید روی و تلقیح بذر با باکتریهای محرک رشد بر فعالیت آنزیم فسفاتاز اسیدی، میزان روی، پروتئین و صفات وابسته به رشد دانه تریتیکاله، آزمایشی با سه تکرار در گلخانه تحقیقاتی دانشکده علوم کشاورزی دانشگاه محقق اردبیلی به صورت فاکتوریل در قالب طرح پایه بلوکهای کامل تصادفی در سال 1392 اجرا گردید. فاکتورهای مورد بررسی شامل محلول پاشی با نانو اکسید روی در پنج سطح (عدم محلول پاشی به عنوان شاهد، 0.25، 0.5، 0.75 و 1 گرم در لیتر) و چهار سطح تلقیح بذر با PGPR (عدم تلقیح بذر با باکتری به عنوان شاهد، تلقیح بذر با ازتوباکتر کروکوکوم استرین 5، آزوسپریلیوم لیپوفروم استرین OF و سودوموناس پوتیدا استرین 9) بودند. مقایسه میانگینها نشان داد که بالاترین فعالیت آنزیم فسفاتاز اسیدی (1.35 نانومول بر دقیقه)، میزان روی (31.4 میلی گرم بر کیلوگرم)، پروتئین (134.2 گرم بر کیلوگرم)، سرعت (0.0017 گرم در روز) و طول دوره پر شدن دانه (40.74 روز) به ترکیب تیماری تلقیح بذر با آزوسپریلیوم در محلولپاشی 1 گرم در لیتر نانو اکسید روی و کمترین آنها ( به ترتیب 0.46 نانومول بر دقیقه، 23.1 میلی گرم بر کیلوگرم، 108.6 گرم بر کیلوگرم ، 0.0012 گرم در روز و 24.14 روز) در عدم تلقیح بذر با باکتریهای محرک رشد × عدم محلول پاشی نانو اکسید روی (شاهد) به دست آمد. به نظر میرسد به منظور بهبود کیفیت بذر تولیدی و افزایش میزان عنصر روی، پروتئین دانه و طول دوره پر شدن دانه میتوان پیشنهاد کرد که تلقیح بذر تریتیکاله با باکتری آزوسپریلیوم در محلول پاشی یک گرم در لیتر نانو اکسید روی به کار برده شود. | ||
| کلیدواژه‌ها | ||
| پر شدن دانه؛ تریتیکاله؛ روی؛ کود های زیستی | ||
| عنوان مقاله [English] | ||
| Effects of Nano-Zinc oxide and plant growth promoting rhizobacteria on zinc and protein content, phosphatase activity and related traits to grain growth of Triticale | ||
| نویسندگان [English] | ||
| H. Kamari1؛ R. Seyed Sharifi2 | ||
| 1Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| 2Professor/ Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran. | ||
| چکیده [English] | ||
| In order to study of the effects ofnano-zinc oxide andseed inoculation with plant growth promoting rhizobacteria on phosphatase activity, zinc and protein content and related traits to grain growth of Triticale, a factorial experiment was conducted based on a randomized complete block design with three replications in research greenhouse of faculty of Agriculture Sciences, University of Mohaghegh Ardabili in 2013. Factors were seed inoculation with plant growth promoting rhizobacteria in four levels (without inoculation as control, seed inoculation with Azotobacter chrocoococum strain 5, Azosprillium lipoferum strain OF and Psedomunas putida strain 9) and foliar application of nano-zinc oxide at five levels (0 as control,0.25, 0.5, 0.75 and 1 g.lit-1). Means comparisons showed that maximum of phosphatase activity (1.35 nmol.min-1, zinc (31.4 mg.kg-1 and protein (134.2 g.kg-1) content, rate (0.10017 g.day-1) and grain filling period (40.74 days)were obtained at application of 1 g.lit-1 Nano-Zinc oxide and seed inoculation with Azosprillium and minimum of them (0.46 nmol.min-1, 23.1 mg.kg-1 ,108.6 g.kg-1, 0.0012 g.day-1 and 24.12 days respectively) were recorded at no application of nano-zinc oxide and without of seed inoculation with PGPR. Therefore, it seems that in order to improve seed quality, increasing of grain filling period, zinc and protein content, it can be suggested that be applied 1 g.lit-1 Nano-Zinc oxide ×seed inoculation with Azosprillium. | ||
| کلیدواژه‌ها [English] | ||
| Grain filling period, Bio fertilizers, triticale, Zinc | ||
| مراجع | ||
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