| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,110 |
| تعداد مقالات | 34,666 |
| تعداد مشاهده مقاله | 49,824,475 |
| تعداد دریافت فایل اصل مقاله | 80,170,457 |
تأثیر پوترسین و تیدیازورون بر اندامزایی درون شیشهای گیاه مریمگلی (Salvia officinalis L.) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 3، دوره 25، شماره 2 - شماره پیاپی 50، مهر 1396، صفحه 201-211 اصل مقاله (538.59 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.113311 | ||
| نویسندگان | ||
| سمیرا جعفری1؛ محمدحسین دانشور2؛ محمدرضا صالحی سلمی* 3؛ امین لطفی جلال آبادی4 | ||
| 1دانشآموخته کارشناسی ارشد گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ایران | ||
| 2استاد، گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ایران | ||
| 3استادیار، گروه علوم باغبانی، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان | ||
| 4استادیار گروه زراعت، دانشگاه کشاورزی و منابع طبیعی رامین خوزستان، ایران | ||
| چکیده | ||
| مریمگلی (Salvia officinalis L.) یکی از با ارزشترین گیاهان تیره نعناع است و بومی ایران، مدیترانه، جنوبشرق آفریقا، آسیای مرکزی و جنوب آمریکا گسترش است. با توجه به قوه نامیه پایین بذر و پرهزینه و وقتگیر بودن تکثیر رویشی آن از یکسو و از سوی دیگر حفظ ذخایر ژرمپلاسم این گیاه دارویی در معرض انقراض، تکثیر انبوه آن توسط تکنیکهای کشت بافت امری ضروریست. در این مطالعه اندامزایی غیرمستقیم مریمگلی مورد بررسی قرار گرفت. ریزنمونههای برگ (5/0 در 5/0 سانتیمتر) و میانگره (1 سانتیمتر)، از گیاهچههای 45 روزه رشد کرده در شرایط درون شیشهای، در محیط کشت موراشیگ و اسکوگ (MS) حاوی 12 ترکیب مختلف از 2,4-D یا NAA بههمراه BAP قرار گرفتند. بهترین کالوسزایی از ریزنمونههای میانگره در محیط کشت MS حاوی 1 میلیگرم در لیتر NAA و 5/0 میلیگرم در لیتر BAP بهدست آمد. بهترین شاخهزایی (66/5 عدد) از کالوسهای بهدست آمده از ریزنمونههای میانگره در محیط کشت MS حاوی 50 میلیگرم در لیتر پوترسین و 5/0 میلیگرم در لیتر تیدیازورون بهدست آمد. بهترین رشد طولی شاخه و ریشهزایی در محیط کشت MS حاوی 1 میلیگرم در لیتر IBA ایجاد شد و گیاهان حاصل در گلخانه سازگار شدند. این روش میتواند بهمنظور باززایی گیاهان پس از انتقال ژن و بدون تغییر شیمری، مورداستفاده قرار گیرد. | ||
| کلیدواژهها | ||
| پلیآمین؛ دارویی؛ ریشهدهی؛ شاخهزایی؛ کالوس؛ کشت بافت | ||
| عنوان مقاله [English] | ||
| Influence of putrescine and thidiazuron on in vitro organogenesis in Salvia officinalis L. | ||
| نویسندگان [English] | ||
| S. Jafari1؛ M.H. Daneshvar2؛ M.R. Salehi Salmi3؛ Amin Lotfi Jalal-Abadi4 | ||
| 1M.Sc., Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran. | ||
| 2Prof., Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran | ||
| 3Assist. Prof., Department of Horticulture Science, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran. | ||
| 4epartment of Agronomy, Ramin University of Agriculture and Natural Resources, Khoozestan, I.R. Iran | ||
| چکیده [English] | ||
| In this study, an indirect organogenesis regeneration protocol for Salvia officinalis L. is described. Leaf (0.5 × 0.5 cm) and internode (1 cm in length) explants sections, from 45 day old in vitro germinated plant, were cultured in Murashige and Skoog (MS) basal medium supplemented with 12 different combinations of 2,4-dichlorophenoxy acetic acid (2,4-D) or 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). The best calli induction response was observed when 1 mg/l NAA and 0.5 mg/l BAP combination was applied to the internode explants. Also, results showed an interesting possibility for the stimulation of shoot regeneration (5.66) through internode segments cultured on MS medium containing 50 mg/l putrescine and 0.5 mg/l TDZ. Shoot elongation and rooting were achieved on MS medium with 1mg/l IBA. Excellent acclimatization to greenhouse conditions was observed for all transferred plantlets. By this procedure no morphological differences were observed between the regenerated and mother plants. This protocol may be also utilized to carry out plant regeneration after genetic transformation, in order to develop transformed plants without the presence of chimeric zones. | ||
| کلیدواژهها [English] | ||
| Callus, Medicinal, Polyamine, rooting, Shoot regeneration, Tissue culture | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
- Alessandra, F.M., Marco, A.O., Armando, R.T., Fabio, V. and Giuseppina, P.P.L., 2009. Endogenous and exogenous polyamines in the organogenesis in Curcuma longa L. Scientia Horticulturae, 121 (4): 501-504. - Ali, R.M., Abbas, H.M. and Kamal, R.K., 2009. The effect of treatment with polyamines on dry matter and some metabolites in salinity stressed chamomile and sweet majoram seedlings. Plant and Soil Environment, 55 (11): 477-483. - Azza, A., Tawfic, M. and Mohamed, F., 2007. Regeneration of Salvia (Salvia officinalis L.) via induction of meristematic callus. In vitro Cellular and Developmental Biology-Plant, 43 (1): 21-27. - Bagni, N. and Torrigiani, P., 1982. Polyamines: A new class of growth substances. Progress in plant growth regulation, 13: 264-275. - Bais, H.P., Sudha, G. and Ravishankar, G.A., 2000. Putrecine and Silver nitrate influence shoot multiplication, in vitro flowering and endogenous titers of polyamines in Cichorium intybus L. cv. Lucknowlocal. Plant Growth Regulator, 19 (2): 238-248. - Baricevic, D., Sosa, S., Della Loggia, R., Tubaro, A., Simonovska, B. and Krasna, A., 2001. Topical inflammatoty activity of Salvia officinalis L. Leaves: the relevance of ursolic acid. Journal of Ethnopharmacology, 75 (2-3): 125-132. - Chien, C.F., Wu, Y.T. and Tasi, T.H., 2011. Biological analysis of herbal medicines used for the treatment of liver diseases. Biomedical Chromatography, 25(1-2): 21-38. - Desai, H.V. and Mehta, A.R., 1985. Changes in polyamine levels during shoot formation, root formation and callus induction in cultured Passiflora leaf discs. Journal of Plant Physiology, 119 (1): 45-53. - Egea-Cortines, M. and Mizrahi, Y., 1991. Polyamines in cell division, fruit set and development, seed germination. In: R.D., Slocum. and H. Flores. (eds.) Biochemistry and Physiology of Polyamines in Plants, 143-158. - Eidi, A., Eidi, M. and Badie, M., 2008. Antinociceptive effect of Salvia officinalis L. leaf essential oil using formalin test in male mice. Journal of Medicinal Plants, 7(4): 94-99. - Ganesan, M. and Jayabalan, N., 2006. Influence of cytokinins, auxins and polyamines on in vitro mass multiplication of cotton (Gossypium hirsutum L. cv. SVPR2). Indian Journal of Exprimental Biology, 44 (6): 506-513. - Ghasemi Lemraski, M., Eftekhari, M., Faraji, M. and Samadi Zarrini, S., 2014. Study of Callus Induction in common sage (Salvia Officinalis L.). International Journal of Agriculture and Crop Sciences, 7(7): 386-389. - Gikloo, T.S., Elhami, B. and Khosrowchahli, M., 2012. Effects of explants type, plant growth regulators and activated charcoal on direct organogenesis of Silybum marianum. African Journal of Biotechnology, 11: 9023– 9027. - Heywood, V.H. 1985. Flowering plants of the world. Oxford University Press. pp 309–311. - Hosseini, B. and Bighamat, A., 2016. Effects of different concentrations of growth regulators and explants type on callus induction, embryogenesis and shoot regeneration of Origanum vulgare ssp. Gracile. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24: 264-276. - Hoseinpanahi, S., Majdi, M. and Mirzaghaderi, Gh., 2016. Effects of growth regulators on in vitro callogenesis and regeneration of black cumin (Nigella sativa). Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24: 232-242. - Huetteman, C.A. and Preece, J.E., 1993. Thidiazuron: A potent cytokinin for woody plant tissue culture. Plant Cell, Tissue and Organ Culture, 33 (2): 105-119. - Kennedy, D.O., Pace, S., Haskell, C., Okello, E.J., Milne, A. and Scholey, A.B., 2006. Effect of cholinesterase inhibiting sage (Salvia officinalis L.) on mood, anxiety and performance on a psychological stressor battery. Neuropsychopharmacology, 31(4): 845-852. - Kintzios, S., Nicolaou, A. and Skoula, M., 1999. Somatic embryogenesis and in vitro rosmarinic acid accumulation in Salvia officinalis and Salvia friticosa leaf callus cultures. Plant Cell Reports, 18 (6): 462-466. - Liu, H., Guoping, Z., Guozheng, S., Songlin, R. and Qiaojuan, F., 2012. Callus induction and plant regeneration from mature seeds of Salvia splendens. International Journal of Agriculture and Biology, 14 (3): 445-452. - Longaray, P., Delamare, A., Moschen-Pistorello, I., Liaane, A., Lusiana, A. and Echeverrigaray, S., 2007. Antibacterial activity of the rssential oils Salvia officinalis L. and Salvia triloba L. cultivated in South Brazil. Food Chemistry, 100 (2): 603-608. - Modares, M., Lahuti, M., Gangali, A. and Asili, G., 2012. Studies micropropagation Salvia leriifolia using cultured. Journal of Plant Biology, 14 (4): 89-100. - Murthy, B.N., Murch, S.J. and Praveen, K., 1998. Thidiazuron: a potent regulator of in vitro plant morphogenesis. Biologica Plantarum, 34 (4): 267-275. - Ndiaye A., Diallo, M.S., Niang, D. and Gassama-Dia, Y.K., 2006. In vitro regeneration of adult trees of Bambusa vulgaris. African Journal of Biotechnology, 5 (3): 1245-1248. - Neena, K. and Pardha Sarathi, P., 1992. Regeneration of plants from callus cultures of Origanum vulgare L. Plant Cell Reports, 11: 476-479. - Nurinas, M., 2004. Inclusion of polyamines in the medium improves shoot elongation in hazelnut (Corylus avellana L.) micropropagation. Turkish Journal of Agriculture and Forestry, 28: 189-194. - Nowruzian, A., Masoumian, M., Ebrahimi, M.A. and Bakhshi Khaniki, G.R., 2016. Micropropagation of Ferula assa-foetida L. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 24: 123-133. - Pandey, A. and Sushma, T., 2012. Influence of kinetin on in vitro rooting and survival of banj oak (Quercus leucotrichophora L.). African Journal of Biotechnology, 1: 12538-12545. - Paschalidis, K.A. and Roubelakis-Anelakis, K.A., 2005. Spatial temporal distribution of polyamine levels and polyamine anabolism in different organs/tissues of the tobacoo plant. Plant Physiology, 138(1): 142-152. - Perry, E.K., Pickering, A.T., Wang, W.W., Houghton, P.J. and Perry, N.S.L., 2005. Medicinal plants and Alzheimer’s disease: from ethnobotany to phytotherapy. Journal of Pharmacological. 51 (5): 527–534. - Pinto, E., Salgueiro, L.R., Cavaleiro, C., Palmeira, A., and Goncalves, J., 2007. In vitro susceptibility of some species of yeasts and filamentous fungi of essential oils of Salvia officinalis. Industrial Crops and Products, 26(2): 135-141. - Quoirin, M., Bittencourt, M.J., Zanette, F. and De Oliveria D.E., 1998. Effect of growth regulators on indirect organogenesis of Acacia mearnsii tissues cultured in vitro. Revista Brasileira de Fisiologia Vegetal, 10: 101-105. - Rajeswari, V. and Paliwal, K., 2008. In vitro adventitious shoot organogenesis and plant regeneration from seedling explants of Albizia odoratissima L.f. (Benth.) In vitro Cellular and Development Biology-Plant 44: 78–83. - Shriram, V., Kumar, V. and Shitole, M.G., 2008. Indirect organogenesis and plant regeneration in Helicteres isora L., an important medicinal plant. In vitro Cellular and Developmental Biology-Plant, 44: 186–193. - Taarit, M.B., Msaada, K., Hosni, K. and Marzouk, B., 2010. Changesin fatty acid and essential oil composition of sage (Salvia officinalis L.) leaves under NaCl strees. Food Chemistry, 119 (3): 959-951. - Tenerife, L., 2004. In vitro callus induction and plants from stem and petiole explants of Salvia canariensis. Plant Tissue Cultre, 14(2): 167-172. - Thiruvengadam, M., Chung, I. and Chun, S., 2012. Influence of polyamines on in vitro organogenesis in bitter melon (Momordica charantia L.). Journal of Medicinal Plants Research, 6(19): 3579-3585. - Tomsone, S. and Gertnere, D., 2003 In vitro shoot regeneration from flower and leaf explants in Rhododendron. Biologia Plantarum, 46 (3): 463-465. - Tripthi, L. and Tripathati, J.N., 2003. Role of biotechnology in mediacinal plants. Pharmaceutical Reserch, 2 (2): 243-253. - Vasudevan, A., Selvaraj, N., Ganapthi, A., Kasthurirengan, S., Ramesh Anbazhagan, V., Manickavasagam, M. and Choi, C.W., 2008. Leucine and spermidine enhance shoot differentiation in cucumber (Cucumis sativus L.). In vitro Cellular and Developmental Biology, 4: 300-306. - Walch, S.G., Tinzoh, L.N., Zimmermann, B.F., Stuhlinger,W. and Lachenmeier, D.W., 2011. Antioxidant capacity and polyphenolic composition as quality Indicators for aqueous infusions of Salvia officinalis L. (sage tea). Frontiers in Pharmacology, 19: 72-79. - Walden, R., Cordeiro, A. and Tiburcio, A.F., 1997. Polyamines: Small molecules triggering pathways in plant growth and development. Plant Physiology, 113: 1009-1013. - Zhu, C. and Chen, Z., 2005. Role of polyamines in adventitious shoot morphogenesis from cotyledons of cucumber in vitro. Plant Cell, Tissue and Organ Culture, 81: 45-53.
| ||
|
آمار تعداد مشاهده مقاله: 1,400 تعداد دریافت فایل اصل مقاله: 987 |
||
