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Detection and Isolation of Mycoplasma capricolum Subspecies Capricolum from East Azerbaijan Sheep Flocks | ||
| Archives of Razi Institute | ||
| مقاله 6، دوره 72، شماره 4، اسفند 2017، صفحه 243-248 اصل مقاله (169.2 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.113303 | ||
| نویسندگان | ||
| A. Jafarizadeh1؛ S.A. Pourbakhsh* 1؛ K. Tadayon2؛ M. Jamshidian1 | ||
| 1Department of Microbiology, Faculty of Veterinary Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Mycoplasma Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran | ||
| چکیده | ||
| Mycoplasma capricolum subspecies capricolum (Mcc) is one of the causative agents of contagious agalactia (CA), which is an important disease in sheep and goats in the Mediterranean and Middle East countries. Mycoplasma agalactiae is the classic agent of CA in sheep and goats. Mycoplasma mycoides subspecies Capri (Mmc), Mycoplasma capricolum subspecies capricolum (Mcc), and Mycoplasma putrefaciens (Mp) produce a clinically similar disease, more often in goats. The aim of the present study was to detect Mcc in sheep flocks in East Azerbaijan Province of Iran. Milk, ear canal, and eye swab samples were collected from 49 sheep flocks with clinical signs of CA or a history of a disease. All the samples were examined using both culture and molecular methods. In the molecular method,positive samples for the Mycoplasma genus were tested for M. mycoides cluster and Mcc. From 272 samples, 67, 87, and 62 samples were shown to be positive using the culture method, polymerase chain reaction (PCR) method, and both culture and PCR methods, respectively. Mcc was detected in all the four M. mycoides cluster positive samples, including milk, ear canal, and eye swab samples. This is the first report of Mcc detection from East Azerbaijan. Our results showed that eye, milk, and ear canal samples could be suitable sources for Mcc detection in sheep flocks. | ||
| کلیدواژه‌ها | ||
| Mycoplasma capricolum subspecies capricolum؛ Sheep؛ Culture؛ PCR؛ East Azerbaijan Province | ||
| مراجع | ||
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A.R, A., 2013. Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran.Arch Raz Inst 68, 11-16. Al-Momani, W., Halablab, M.A., Abo-Shehada, M.N., Miles, K., McAuliffe, L., Nicholas, R.A., 2006. Isolation and molecular identification of small ruminant mycoplasmas in Jordan. Small Rumin Res 65, 106-112. Awan, M., Abbas, F., Yasinzai, M., Nicholas, R., Babar, S., Ayling, R., et al., 2009. Prevalence of Mycoplasma Capricolum Subspecies Capricolum and Mycoplasma Putrefaciens in Goats in Pishin District of Balochistan . Pak Vet J 29. Benkirane, A., Amghar, S., Kirchhoff, H., 1993. Analysis of Membrane Proteins of Mycoplasma capricolum Strains by SDS‐PAGE and Immunoblotting. Zoonoses Public Health 40, 119-124. Borry, G., Entessar, F., 1963. Etude sur l agalaxie des chevre et des moutens. Arch Raz Inst 15, 45-63. Cordy, D., Adler, H., Yamamoto, R., 1955. A pathogenic pleuropneumonialike organism from goats. Cornell Vet 45, 50-68. Corrales, J., Esnal, A., De la Fe, C., Sánchez, A., Assunçao, P., Poveda, J., et al., 2007. Contagious agalactia in small ruminants. Small Rumin Res 68, 154-166. De la Fe, C., Gutierrez, A., Poveda, J., Assuncao, P., Ramírez, A., Fabelo, F., 2007. First isolation of Mycoplasma capricolum subsp. capricolum, one of the causal agents of caprine contagious agalactia, on the island of Lanzarote (Spain). The Vet J 173, 440-442. Gómez-Martín, Á., Amores, J., Paterna, A., De la Fe, C., 2013. Contagious agalactia due to Mycoplasma spp. in small dairy ruminants: Epidemiology and prospects for diagnosis and control. The Vet J 198, 48-56. Heller, M., Schwarz, R., Noe, G., Jores, J., Fischer, A., Schubert, E., et al., 2015. First human case of severe septicaemia associated with Mycoplasma capricolum subsp. capricolum infection. JMM Case Reports 2. Hotzel, H., Sachse, K., Pfützner, H., 1996. A PCR scheme for differentiation of organisms belonging to the Mycoplasma mycoides cluster. Vet Microbiol 49, 31-43. Kheirkhah, B., Pourbakhsh, S.A., Nadalian, M.-g., Banani, M., Ashtari, A., 2011. Detection of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) methods from Iranian goats. Afr J Microbiol Res 5, 1668-1972. Khezri, M., Pourbakhsh, S., 2014. A survey of Mycoplasma agalactiae in small ruminants with contagious agalactiae syndrome in Iran. Bang J Vet Med 12, 67-72. Khezri, M., Pourbakhsh, S., Ashtari, A., Rokhzad, B., 2015. Investigation of Mycoplasma agalactiae in sheep in Kurdistan province by PCR. Iran.Arch Raz Inst 70, 73-80. May, M., Balish, M.F., Blanchard, A., 2014. The Order Mycoplasmatales. The Prokaryotes, Springer, pp. 515-550. Moslemi, M., Pourbakhsh, S., Jamshidian, M., Tadayon, K., 2013. Isolation and molecular detection of M. mycoides Nicholas, R., Ayling, R., McAuliffe, L., 2008. Mycoplasma diseases of ruminants, CABI. Ostrowski, S., Thiaucourt, F., Amirbekov, M., Mahmadshoev, A., Manso-Silván, L., Dupuy, V., et al., 2011. Fatal Outbreak of mycoplasma capricolum pneumonia in endangered markhors. Emerging infectious diseases 17, 2338. Pooladgar, A., Looni, R., Ghaemmaghami, S., Pourbakhsh, A., Ashtari, A., Shirudi, A., 2015. Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from affected sheep to contagious agalactia of Khuzestan province, Iran. Arch Raz Inst Institute 70, 21-27. Pourbakhsh, S., Abtin, A., Ashtari, A., Bayatzadeh, M., Barani, S., Asli, E., 2015. Detection of Mycoplasma capricolum capricolum from goats of Qom province, Iran. Arch Raz Inst 70, 45-50. | ||
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