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شناسایی ساختار شیمیایی و بیوشیمیایی بذر گیاه دارویی بالنگو شیرازی (Lallemantia royleana (Benth.) Benth. in Wall) و بررسی تغییرات این صفات تحت شرایط پیری تسریع شده | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 6، شماره 1 - شماره پیاپی 11، خرداد 1396، صفحه 23-37 اصل مقاله (757.89 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2017.113285 | ||
| نویسندگان | ||
| محمدشاهین دانشمندی* 1؛ رضا توکل افشاری2؛ رضا صدرآبادی حقیقی3 | ||
| 1محقق ارشد بیولوژی، بیوشیمی و بیوفیزیک بذرهای دارویی/ دانشکده کشاورزی و منابع طبیعی، دانشگاه تربت حیدریه، ایران. | ||
| 2عضو هیئت علمی/ گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه فردوسی، مشهد، ایران. | ||
| 3عضو هیئت علمی/ گروه زراعت و اصلاح نباتات، واحد مشهد، دانشگاه آزاد اسلامی، مشهد، ایران. | ||
| چکیده | ||
| این پژوهش جهت شناسایی ساختارهای شیمیایی و بیوشیمیایی روغن بذر بالنگو شیرازی (Lallemantia royleana) و بررسی تغییرات این صفات تحت شرایط پیری تسریع شده با مدت زمان 24، 48 و 72 ساعت با رطوبت نسبی 100 درصد و دمای 41 درجه سانتی‌گراد در قالب طرح آماری کاملاً تصادفی بررسی شد. نتایج نشان داد بذر این گیاه دارویی دارای 19.26 درصد روغن است. پروفایل اسیدهای چرب روغن شامل 90.71 درصد اسیدهای چرب غیراشباع در مقابل 9.29 درصد اسیدهای چرب اشباع بود که اسید لینولنیک (C18:3)، اسید اولئیک (C18:1) و اسید پالمتیک (C16:0) به ترتیب مهمترین اسید چرب چند غیر اشباع، تک غیر اشباع و اسید چرب اشباع روغن بودند. آنتی اکسیدان‌های بذر بالنگو شیرازی شامل 427.8 پی‌پی‌ام توکوفرول و210 میلی‌گرم پلی‌فنل بود. شرایط پیری تسریع شده محتوای روغن بذر را تا 15.2 درصد کاهش داد همچنین بطور نسبی باعث افزایش اسیدهای چرب اشباع در مقابل اسیدهای چرب غیراشباع گردید. اوج اکسیداسیون و تولید پراکسید بین شاهد و تیمار پیری تسریع شده 24 ساعت رخ داد. حداکثر مقاومت روغن (رنسیمت) در تیمار پیری تسریع شده 72 ساعت تا 5/1 برابر کاهش پیدا کرد. نتایج نشان داد آزمون‌های جوانه‌زنی تحت تاثیر پیری تسریع شده به صورت خطی کاهش پیدا کرد. ارزش جوانه‌زنی در شاهد به ترتیب 6/1، 5/2 و 8/4 برابر بیشتر از سایر تیمارها بود. دستاوردهای این تحقیق نشان داد اکسیداتیو آنزیمی علاوه بر افزایش سرعت زوال، می‌تواند باعث تغییر ماهیت شیمیایی و بیوشیمیایی ترکیبات ذخیره‌ای بذر بالنگو شیرازی گردد. | ||
| کلیدواژه‌ها | ||
| ارزش جوانه‌زنی؛ اکسیداتیو آنزیمی؛ اسید چرب؛ توکوفرول؛ پراکسید؛ رنسیمت | ||
| عنوان مقاله [English] | ||
| Identification of Chemical and Biochemical Characteristics of Balangu Seeds (Lallemantia royleana (Benth.) Benth. in Wall) under Accelerated Aging Conditions | ||
| نویسندگان [English] | ||
| M.Sh. Daneshmandi1؛ R. Tavakkol Afshari2؛ R. Sadrabadi Haghighi3 | ||
| 1Lecturer/ Faculty of Agriculture and Natural Resources, University of Torbat Heydarieh, Torbat Heydarieh, Iran. | ||
| 2Professor/ Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| 3Professor/ Department of Agronomy and Plant Breeding, Mashhad Branch, Islamic Azad University, Mashhad, Iran | ||
| چکیده [English] | ||
| In this study the identification of chemical and biochemical characteristics of balangu seed oil (Lallemanthia royleana) and their parameters were investigated under accelerated aging conditions at 24 h, 48 h and 72 h at 41°C and 100% RH in a completely randomized design. The results showed that balangu seed oil content was 19.26%.Fatty acid profiles were 90.71% of unsaturated fatty acids (USFA) and 9.29% of saturated fatty acids (SFA), also the linolenic acid (C18:3), oleic acid (C18:1) and palmitic acid (C16:0) were important of PUSFA, MUSFA and SFA oil seeds, respectively.The antioxidants of oil seeds include 427.8 ppm tocopherols and 210 ml.l-1 of polyphenols. The results showed that 15.2% reduction in oil seed under accelerated aging treatment, also this treatments was the cause of increase of SFA compared to USFA. The oxidation and peroxide production peak was between control and 24 h of accelerated aging treatment. Oil resistance (rancimat) in the 72 h of aging treatment was 1.5 times less than to the control. The germination value (GV) was 1.6, 2.5 and 4.5 higher than the accelerated aging treatments, respectively. In general, it is likely that the changes of chemical and biochemical characteristics of seed oil is happened by oxidative enzymes and this condition is quicker in deteriorated balangu seeds | ||
| کلیدواژه‌ها [English] | ||
| fatty acids, Germination Value, Oxidative Enzymes, PEROXIDE VALUE, RANCIMAT, Tocopherols | ||
| مراجع | ||
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