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ارزیابی ارتباط بین چند شکلی پنج جایگاه‌های ریزماهواره‌ای واقع بر کروموزم شماره شش با صفت چند قلوزایی در بز نژاد کشمیر | ||
| علوم دامی | ||
| مقاله 23، دوره 30، شماره 115، شهریور 1396، صفحه 303-316 اصل مقاله (1.07 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2017.113283 | ||
| نویسندگان | ||
| رحمان حاجی علیزاده ولیلو1؛ آرش جوانمرد1؛ نادر اسدزاده* 2 | ||
| 1گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز | ||
| 2استادیار موسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| چکیده | ||
| برای ارزیابی ارتباط بین چندشکلی پنج جایگاه ریزماهواره واقع بر کروموزم شماره شش با صفت چندقلوزایی بز نژاد کشمیر، تعداد 65 رأس بز ماده به‌طور تصادفی از گله 600 رأسی انتخاب و عواملی همچون اطلاعات شجره، اثرات ثابت رکورد‌برداری شده همچون سن مادر، تعداد شکم زایش به همراه تعداد فرزند در هر زایش،اطلاعات تغذیه‌ای و اطلاعات آزمایشات فیزیولوژی تولیدمثل مانند سابقه استفاده از هورمون، به‌همراه صفت هدف چندقلوزایی ثبت شد. خون‌گیری از ورید وداجی و استخراج DNA انجام شد. برنامه تکثیر انفرادی جایگاه‌ها در روی ژل اکریلامید الکتروفورز و تجزیه و تحلیل شد. وجود چندشکلی در چهار جایگاه را تأیید کرد و جایگاه BM4621 الگوی مونومورف را نشان داد. رابطهی آماری چند شکلی جایگاه‌های مورد مطالعه با صفت تعداد نتاج در اولین سال زایش معنیدار بود (01/0>p). میانگین تعداد بزغالهی متولد شده از بزهای حاوی ژنوتیپهای 180/180و180/188 برای جایگاه BM1329، ژنوتیپهای 112/110 و 112/112برای جایگاه OarAE101 ، ژنوتیپهای 108/106 و 108/108برای جایگاه BMP143 و در نهایت ژنوتیپ 200/200 برای جایگاه BMP143 بالاتر از سایر ژنوتیپهای بود (01/0>p). روابط آماری معنیدار شناسایی شده در این مطالعه می‌تواند به منظور بهبود راندمان تولیدمثلی بر مبنای انتخاب به کمک نشانگر و همچنین در فرایند غربال‌گری برای شناسایی بزهای ماده چندقلوزا به کار گرفته شود. | ||
| کلیدواژه‌ها | ||
| "بز"' "چندقلوزایی"؛ "نشانگر ریزماهواره"؛ "کروموزوم 6" | ||
| عنوان مقاله [English] | ||
| Association between Microsatellite Markers of Chromosome 6 and Litter size of Cashmere Goats | ||
| نویسندگان [English] | ||
| Rahman Hajializadeh Valilou1؛ Arash Javanmard1؛ nader asadzadeh2 | ||
| 1Graduated from Tabriz university | ||
| 2Assistant professor of Animal Science Research Institute of Iran | ||
| چکیده [English] | ||
| Five microsatellite markers BM1329, OarAE101, BM143, BM4621 and BM415 which were closely linked to the fecundity gene FecB in Booroola sheep were chosen based on their high conservatism in the closest species and were analyzed for polymorphisms and also their correlation with the litter size of a goat. Observed results showed four microsatellite loci show polymorphism in the present study. However, BM4621 locus was monomorphic. The range of allele size of BM1329, OarAE101, BM143 and BM415 were 180 to 216 to 112, 102 to 112 and 198 to 200 in goat respectively. The alleles of the greatest frequency for of BM1329, OarAE101, BM143 and BM415 were 212, 99, 108 and 200bp respectively. Polymorphism information content (Nei Index) for BM1329, OarAE101, BM143 and BM415 were 0.82, 0.82, 0.72 and 0.49 respectively. Mean number of allele, Observed Heterozygosis, PIC and Shannon information index were 4.75 ± 2.2, 0.36 ± 0.12, 0.71 ± 0.15 and 1.41 ± 0.52 respectively. The litter size for first lambing for investigated loci were significantly different (p<0.01). The total mean of litter size of genotype BM1329 180/180, 180/188, oarAE101 110/112, 112/112, BMp143 106/108, 108/108 and BMP143 200/200bp were significantly higher than of other genotypes. This association could be further evaluated for marker-assisted selection and developed PCR methodology would expedite screening for large number of goats required for such studies. | ||
| کلیدواژه‌ها [English] | ||
| "Goat", " Litter Size", "Microsatellite", "Chromosome 6" | ||
| مراجع | ||
|
Banabazi, M. H., Mirai Ashtiani, S. R., Moradi Shahrbabak, M. & Esmailkhanian, S. (2006). Study of genetic diversity within and between five Iranian sheep populations using microsatellite markers. Journal of Science and Technology Agriculture and Natural Resources, 10(4), 481-488. (In Farsi). Beaumont, M. A., and Bruford, M. W. (1999). Microsatellites in conservation genetics. In D. B. Goldstein and C. Schlotterer (Eds.), Microsatellites: Evolution and applications (pp. 165/180). Oxford: Oxford University Press. Bindon, B.M. and Piper, L.R. (1986). The reproductive biology of prolific sheep breeds. Oxford Rev. Reprod. Biol., l8: 414 Chemineaup., G. Barila, B. Leboeufb, M.C. Maurela and Y. Cogniea.(1998 ).Recent advances in the control of Goat reproduction. ressources.ciheam.org Chu, M. X., Jiao, C. L., He, Y. Q., Wang, J. Y., Liu Z. H. and Chen, G. H. (2007). Association between PCR/SSCP of bone morphogenetic protein 15 gene and prolificacy in Jining Grey goats. Anim Biotechnol.18(4): 263/74. Davis, G. H., Balakrishnan, L., Ross, I. K., Wilson, T., Galloway, S. M., Lumsden, B. M., Hanrahan, J. P., Mullen, M., Mao, X. Z., Wang, G. L., Zhao, Z. S., Zeng, Y. Q., Robinson, J. J., Mavrogenis, A. P., Papachristoforou, C., Peter, C., Baumung, R., Cardyn, P., Boujenane, I., Cockett, N. E., Eythorsdottir, E., Arranz, J. J. and Notter, D. R. (2006). Investigation of the Booroola (FecB) and Inverdale (FecX(I)) mutations in 21 prolific breeds and strains of sheep sampled in 13 countries. Anim Reprod Sci.92(1/2): 87/96. Fitz Simmons, N. N., Tanksley, S., Forstner, R.J., Louis, E.E., Daglish, R., Gratten, J. and Davis, S. (2000). Microsatellite markers for Crocodylus: New genetic tools for population genetics, mating system studies and forensics. In G. C. Grigg, F. Seebacher, and C. E. Franklin (Eds.). Crocodilian biology and evolution (pp. 51/57). Chipping Norton: Surrey Beatty and Sons. Gootwine, E., Reicher, S. and Rozov, A. (2007). Prolificacy and lamb survival at birth in Awassi and Assaf sheep carrying the FecB (Booroola) mutation. Anim Reprod Sci. Hagger, By C. (2000).Genetic and environmental influences on size of first litter in sheep, estimated by the REML method. Journal of Animal Breeding and Genetics, Volume 117, Number 1, Febuary 2000, pp. 57/64(8) Hou, J.X., Wang, J.G., An, X.P., Zhu, G.Q. and Cao, B.Y. (2014). Polymerisation effects of four microsatellites on litter size in Xinong Saanen goats. Animal Production Science. 55: 1051-1055 Hua, G. H., Chen, S. L., Ai, J. T. and Yang, L. G. (2007). None of polymorphism of ovine fecundity major genes FecB and FecX was tested in goat. Anim Reprod Sci. Jean/Marie Luginbuhl. (2002). Breeds and Production traits of meat goats. North Carolina State University, College of Agriculture and Life Sciences. www.cals.ncsu.edu/an_sci/extensio Lord, E.A., Lumesden, J.M.,Dodds, K.G., Henry,H.M., Crawford, A.M., Ansari, H.A.,PEARCE, P.D., Maher, D.W., Stone, R.T., Kappes, S.M., Beattie, C.W. and Montegomery, G.W.(1996). The linkage map of sheep chromosme 6 compared with orthologous regions in other species.Mammalian Genome 7, 373-376 Maddox ,J.F., Davies, K.PCrawford, A.M., Hulme, D.J., Vaiman, D., Cribiu, E.P., Freking, B.A., Beh, K.J., Cockett, N.E., Kang, N., Riffkin, C.D. Drinkwater, R., Moore, S.S., Dodds, K.G., Lumsden, J.M. van Stijn, T.C., Phua, S.H., Adelson, D.L., Burkin, H.R., Broom, J.E., Buitkamp, J., Cambridge, L., Cushwa, W.T., Gerard, E.,Galloway, S.M. Harrison, B., Hawken, R.J., Hiendleder, S., Henry, H.M., Medrano, J.F. Paterson, K.A., Schibler, L.,Stone, R.T. and van Hest, B. (2001). An enhanced linkage map of the sheep genome comprising more than 1000 loci. Genome Res. 11 pp. 1275–1289. Montgomery, G.W., Crawford, A.M., Penty, J.M., Dodds, K.G., Henry, H.M., Pierson, A.J., Lord, E.A., Galloway, C.A., Schmack, A.E., Sise, J.A., Swarbrick, P.A., Hanrahan, V., Buchanan , F.C. and Hill, D.F. (1993). The ovine Booroola fecundity gene (FecB) is linked to markers from a region of human chromosome 4q. Nat. Genet., 4: 410–414. Montgomery, G.W., Lord, E.A., Penty, J.M., Dodds, K.G., Broad, T.E., Cambridge, L., Sunden, S.L., Stone, R.T and Crawford, A.M. (1994). The Booroola fecundity (FecB) gene maps to sheep chromosome 6. Genomics. Jul 1;22(1):148–153. Nejadgashti, M. (2004). Molecular study of some microsatellite markers in six Iranian native goat populations (Markhaz, Lori, Najdi, Tali, Raeini and Kordi). M.Sc. Thesis. Faculty of Agriculture. Islamic Azad University-Karaj Branch. (In Farsi). 19. Ott, J. (1988–2001). Program Het version 1.8. Ouyang Xu/Xiang , Si Qi/Shun, Huang Sheng/Qiang, Deng Zao/Fu, Liu He /Xiang, He De/Shi, Tan Sheng/Guo and Hu Shu/Guang.(2006). Analysis of microsatellite DNA markers of OarHH35 and BMS2508 in four goat breeds. Chinese Journal of Agricultural Biotechnology (2006), 3: 217/222 Cambridge University Press. PIPER, L. R. Bindon, B. M. and Davis, G. H. (1985). The single gene inheritance of the high litter size of the Booroola Merino. In: Land, R.B., Robinson, D.W. (Eds.), Genetics of Reproduction in Sheep. Butterworths, London, p. 115–125. Qanbari, S., R. Osfoori, M. P. Eskandari Nasab. (2007) Introgression of major genes into elite breeding flock of afshari sheep: a preliminary evaluation of polymorphism content and applicability of marker data. Biotechnology. 6: 513-519 Schibler, S. L., Cribiu, E.P., Oustry/Vaiman, A., Furet, J.P. and Vaiman, D. (2002). Fine mapping suggests that the goat Polled Intersex Syndrome and the human Blepharophimosis Ptosis Epicanthus Syndrome map to a 100/kb homologous region. Genome Res. 10 (2000), pp. 311–318 Stephan,Wildeus.(1998). Reproductive management of the meat goat. www.goatworld.com/articles/pregnancy Wang, J.G., Hou, J.X, Li, Guang., Qin Zhu, G. and Cao, B.Y. (2013). Polymorphism of four microsatellites and their polymerisation effect on litter size in Boer goats. Electronic Journal of Biotechnology. 16: 1-10. Wang, Y., Nana, Z., Zhanbin, W., Qingyi, W., Xiaohui, Z., Junyan, B. and Youzhi, P.(2010). Association of Polymorphism of Microsatellite Markers with Litter Size in Chinese Funiu White Goat. Research Journal of Animal Sciences. 4: 92-98. Xu, Y. X., Chen, S. L., Wen, Q. Y., Shen, Z., Zhang, C. Y., Yao, H. W., Hua, G. H. and Yang , L. G.(2007). Correlation analysis between 9 microsatellite markers and fecundity trait of Boer goat. Yi Chuan.29(11): 1385/92. Zhu, G.Q., Cui, Y.H., Song, Y.X., Wang, J.G. and Cao, B.Y.(2011). Screening of seven microsatellite markers for litter size in Xinong Saanen dairy goat. African Journal of Biotechnology. 10: 8523-8528. Zuzanna,N., Krystyna, M. and Charon. (2001).Identification of fecundity gene (FecB) carriers using microsatellite markers and its effect on sheep weight. Journal of Applied Genetics. 42(1),pp. 49 / 57. | ||
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