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مقایسه مقادیر فنل کل در بافت‌های برگ و میوه ژنوتیپ‌های به و بررسی تأثیر آن در مقاومت به بیماری آتشک | ||
| نهال و بذر | ||
| مقاله 4، دوره 32، شماره 3، آذر 1395، صفحه 331-345 اصل مقاله (248.54 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/spij.2016.113062 | ||
| نویسندگان | ||
| الناز قضاتی1؛ حمید عبداللهی* 2؛ سعید پیری1 | ||
| 1دانشگاه آزاد اسلامی، واحد ابهر، دانشکده کشاورزیو منابع طبیعی، ابهر | ||
| 2پژوهشکده میوه‌های معتدله و سردسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران | ||
| چکیده | ||
| فنل‌ها از متابولیت‌های ثانویه مؤثر در تحمل به تنش‌های محیطی در گیاهان است و بافت‌های برگ و میوه درخت به منبع مهمی از این گروه ترکیبات محسوب می‌شوند. پژوهش اخیر با هدف بررسی میزان و تغییرات فنل در بافت‌های برگ و میوه ژنوتیپ‌های به منطقه اصفهان و ارتباط آن با تحمل به بیماری آتشک انجام شد.برای این منظور، دوازده ژنوتیپ گزینش شدهKVD4،SHA1 ، KVD3، PH2، ET1، NB3، KVD1، KM1، SVS1، SVS2،NB2 و شاهد رقم اصفهان، مورد ارزیابی قرار گرفتند. عصاره‌های اتانولی از نمونه‌های برگ در بهار و تابستان واز میوه‌های رسیده در ابتدای پاییز تهیه و با استفاده از معرف فولین سیکالتیو میزان فنل کل بر حسب معادل اسید گالیک اندازه‌گیری شد. نتایج بیانگر این بود که ژنوتیپ‌های مورد مطالعه از نظر میزان فنل کل برگ دارای تفاوت معنی‌داری بودند. ژنوتیپ KVD4 و رقم اصفهان با مقادیر 9/14و 7/2 میلی‌گرم اسیدگالیک برگرم بافت تازه برگ به ترتیب بیش‌ترین و کم‌ترین میزان فنل کل را داشتند. برگ‌های ژنوتیپ‌های به، افزایش پیوسته‌ای را در مواد فنلی تا انتهای تابستان نشان دادند.برخلاف نمونه‌های برگی، تفاوت معنی‌داری در میزان فنل کل میوه‌ها در ارقام و ژنوتیپ‌های مورد بررسی مشاهده نشد. ارزیابی تأثیر میزان فنل به عنوان یکی از مهم‌ترین آنتی‌اکسیدان‌های غیر‌آنزیمی روی توسعه بیماری آتشک، بیانگر همبستگی منفی شاخص حساسیت واریته‌ای (I.V.S) با میزان فنل کل در برگ‌های ابتدای تابستان در ژنوتیپ‌های به مورد بررسی بود. | ||
| کلیدواژه‌ها | ||
| Cydonia oblonga Mill؛ اسید گالیک؛ Erwinia amylovora؛ فنل؛ آتشک | ||
| عنوان مقاله [English] | ||
| Comparison of Total Phenolic Contents of the Leaves and Fruits of Quince Genotypes and its Effects on the Fire Blight Resistance | ||
| نویسندگان [English] | ||
| E. Ghozati1؛ H. Abdollahi2؛ S. Piri1 | ||
| 1respectively, Faculty of Agriculture and Natural Resources, Abhar Branch, Islamic Azad University, Abhar, Iran. | ||
| 2Temperate Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran. | ||
| چکیده [English] | ||
| Phenols are important secondary metabolites with important role in tolerance to the environmental stresses, and quince leaves and fruits are significant sources of phenolic compounds. The current research was conducted to study the contents and variations of total phenolic compounds in the leaves and fruits tissues of selected quince genotypes from Isfahan and investigate its eventual role in the fire blight resistance in this species.Twelve quinces genotypes, KVD4, SHA1, KVD3, PH2, ET1, NB3, KVD1, KM1, SVS1, SVS2, NB2 and cv. Isfahan as control were evaluated.The ethanol extracts of the leaves and fruits were prepared during spring and summer and total phenol contents were determined by the folin-ciocalteu method, based on equivalent gallic acid. The results showed differences in phenolic compounds in the leaves, of the genotypes, so that genotype KVD4 and cv. Isfahan, with 17.8 and 2.7 mg gallic acid/g fresh weight of the leaves, had the highest and lowest total phenols, respectively. Also a constant increase was observed in the phenol contents of the leaves up to end of the summer. On the other hand, no significant difference was found between the amounts of total phenols in fruits of genotypes. The evaluation of phenol content as major components of non-enzymatic anti-oxidative defense system, against fire blight invasion showed negative correlation of Isv, with phenolic contents of the quince leaves from early summer. | ||
| کلیدواژه‌ها [English] | ||
| Cydonia oblonga, gallic acid, Erwinia amylovora, phenol, fire blight | ||
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