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بررسی آزمایشگاهی اثرات هم افزایی (سینرژیک) مخلوط های آفت کش های آلی، مواد کیلیت کننده فلز و آنتی اکسیدان در برابر قارچ مولد پوسیدگی سفید رنگین کمان | ||
| تحقیقات علوم چوب و کاغذ ایران | ||
| مقاله 11، دوره 32، شماره 3 - شماره پیاپی 60، آبان 1396، صفحه 422-435 اصل مقاله (641.34 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijwpr.2017.114464.1424 | ||
| نویسندگان | ||
| لطیف نظری1؛ سید خلیل حسینی هاشمی* 2 | ||
| 1گروه علوم و صنایع چوب و کاغذ، واحد کرج، دانشگاه آزاد اسلامی واحد کرج، کرج، ایران | ||
| 2دانشیار، گروه علوم و صنایع چوب و کاغذ، واحد کرج، دانشگاه آزاد اسلامی، کرج، ایران | ||
| چکیده | ||
| در این تحقیق اثرات مستقل و متقابل آفت کش های آلی پروپیکونازول، تبوکونازول و کلروتالونیل با کیلیت کننده فلز اتیلن دی آمین تترا استیک اسید (EDTA) و آنتی اکسیدان هیدروکسی تولوئن بوتیل دار (BHT) در غلظت های مختلف 50، 150، 250، 350 و ppm 450 در برابر قارچ مولد پوسیدگی سفید رنگین کمان (Trametes versicolor) مورد آزمون قرار گرفت. در زیر هود لامینار، به میزان 25 میلی لیتر از محیط کشت استریل در داخل پتریدیش ها ریخته شد. سه دیسک آنتی بیوگرام استریل (با موقعیت مثلثی شکل) روی محیط کشت قرار داده شد. به کمک نمونه بردار به میزان 20 میکرولیتر محلول حفاظتی استریل شده بر روی هر یک از دیسک ها به طور جداگانه ریخته شد. سپس به مقدار 5 میلی متر از پلاگ میسیلیوم قارچ در مرکز پتریدیش ها قرار داده شد. پتریدیش ها به مدت یک هفته در داخل انکوباتور قرار داده شدند. روزانه به مدت یک هفته، رشد میسیلیوم قارچ و اثرات بازدارندگی محلول های حفاظتی مختلف در برابر قارچ، مورد اندازه گیری قرار گرفت. نتایج حاصل از این تحقیق نشان داد که آفت کش کلروتالونیل به تنهایی هیچ اثر بازدارندگی بر روی رشد قارچ نداشته است، در حالی که آفت کش های پروپیکونازول و تبوکونازول به تنهایی اثر معنی داری در غلظت های مختلف بر روی رشد قارچ داشته اند. در بررسی اثر هم افزایی (سینرژیک) آفت کش های پروپیکونازول، تبوکونازول و کلروتالونیل با افزودنی های غیر آفت کش EDTA و BHT این طور یافته شده است که بین آفت کش پروپیکونازول و کیلیت کننده فلز EDTA در غلظت بالا مانند ppm 450 اثر هم افزایی وجود داشته است، اما در ترکیب با بقیه محلول ها، اثر هم افزایی یافته نشده است. همچنین، هیچگونه اثر هم افزایی بین آفت کش های تبوکونازول و کلروتالونیل با سایر محلول های حفاظتی یافته نشده است. | ||
| کلیدواژهها | ||
| آفت کش های آلی؛ BHT؛ EDTA؛ بازدارندگی رشد؛ هم افزایی؛ قارچ رنگین کمان | ||
| عنوان مقاله [English] | ||
| Laboratory Study of Synergistic Effects of Mixtures of Organic Biocides, Metal Chelator and Antioxidant Materials against Rainbow White-rot Fungus | ||
| نویسندگان [English] | ||
| Latif Nazari1؛ Seyyed Khalil Hosseinihashemi2 | ||
| 1Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| 2Associate Prof., Department of Wood Science and Paper Technology, Karaj Branch, Islamic Azad University, Karaj, Iran | ||
| چکیده [English] | ||
| In this research, the individual and interaction effects of three organic biocides (propiconazole, tebuconazole, and chlorothalonile) with the metal chelator EDTA and the antioxidant BHT at different concentrations (50, 150, 250, 350, and 450 ppm) against the white-rot fungus, Trametes versicolor was investigated. In under laminar sterile hood, about 25 mL of the media was poured into the Petri plates. Three antibiogram discs (with triangular position) put on the media. By micro-sampler about 20 μL of steriled preservative solutions was poured on every disc, separately. Then, about 5 mm of mycelium plug of intended fungus that was inoculated previously was put in the center of plates. The plates were incubated and daily for one week, the mycelium growth of fungus and inhibition effects of different preservative solutions against fungus was measured. The results of this research demonstrated that chlorothalonil biocide by itself have not any inhibition effect on growth of fungus, whilst propiconazole and tebuconazole biocides by itself have significant effect at different concentrations on growth of fungus. In consider of synergistic effect of propiconazole, tebuconazole, and chlorothalonil biocides with non-biocides additives of EDTA and BHT, it has been found that there is synergistic effect between propiconazole biocide and the metal chelator EDTA at high concentration such as 450 ppm, but in combination with other solutions synergistic effect has not been found. Also, any synergistic effect has not found between tebuconazole and chlorothalonil biocides with other preservative solutions. | ||
| کلیدواژهها [English] | ||
| Organic biocides, EDTA, BHT, growth inhibition, Synergism, TRAMETES VERSICOLOR | ||
| مراجع | ||
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