| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,846 |
| تعداد مقالات | 32,300 |
| تعداد مشاهده مقاله | 41,387,167 |
| تعداد دریافت فایل اصل مقاله | 18,562,195 |
جداسازی باکتری‌های دارای خاصیت quorum quenching با قابلیت بیوکنترل Pectobacterium carotovorum subsp. carotovorum | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 3، دوره 4، شماره 2 - شماره پیاپی 8، اسفند 1395، صفحه 25-38 اصل مقاله (265.74 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2017.112889 | ||
| نویسندگان | ||
| محمد رضا عالی منش1؛ پریسا طاهری* 2؛ سعید طریقی3 | ||
| 1گروه بیماری شناسی گیاهی دانشگاه فردوسی مشهد | ||
| 2دانشیار/ گروه گیاه پزشکی دانشکده کشاورزی دانشگاه فردوسی مشهد | ||
| 3گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد | ||
| چکیده | ||
| تعداد 177 جدایه باکتری کونچر به دو روش استفاده از محیط کشت حداقلی حاوی سیگنال آسیل همو سرین لاکتون از نوع 3-oxo-C6-HSL و محیط کشت‌های عمومی از گیاهان تیره‌ی سولاناسه جداسازی شدند. از بین آنها 11 جدایه متعلق به جنس‌های Pseudomonas، Enterobacter،Acinetobacterو Bacillus فعالیت کونچری بالایی داشتند. بهویژه جنس Pseudomonas که دارای بالاترین میزان فعالیت بود. بررسی سازوکار کونچری در این باکتری‌ها نشان داد که آنزیم‌ها و ترکیبات کوچک غیرآنزیمی با فعالیت کونچری در این فرایند نقش دارند. بیشتر این باکتری‌ها فقط دارای فعالیت آنزیمی بودندکه توسط آزمون HPLC تأیید شدند. همچنین برای اولین‌بار جدایه‌ای از باکتری calcoaceticus Acinetobacterکه با روش دوم جداسازی شده بود با توانایی کونچری غیرآنزیمی شناسایی شد. باکتری‌های کونچر عمدتاً باعث کاهش بیوفیلم و کاهش بیماری‌زایی Pectobacterium carotovorum subsp. carotovorumروی سیب‌زمینی و کاکتوس شدند. بنابراین، رابطه‌ی مستقیمی بین افزایش فعالیت کونچری و توانایی بیوکنترلی علیه این بیمارگر وجود داشت، هر چند موارد استثناء نیز مشاهده شد. همچنین، برخی جدایه‌های Pseudomonasبه‌عنوان بهترین عوامل بیوکنترل که براساس فعالیت کونچری علیه بیمارگر مذکور عمل می‌کردند، تشخیص داده شدند. | ||
| کلیدواژه‌ها | ||
| آسیل‌همو‌سرین لاکتون؛ Acinetobacter calcoaceticus؛ بیوفیلم؛ فعالیت کونچری؛ توانایی بیوکنترلی | ||
| عنوان مقاله [English] | ||
| Isolation of the bacteria with quorum quenching activity against Pectobacterium carotovorum subsp. carotovorum | ||
| نویسندگان [English] | ||
| Mohammad Reza Alymanesh1؛ Parissa Taheri2؛ Saeed Tarighi3 | ||
| 2Associate professor/Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad | ||
| چکیده [English] | ||
| One hundred seventhy seven quorum quenching bacteria were isolated from Solanaceae family using two methods of minimal medium containing synthetic acyl homoserine lactone signal 3-oxo-C6-HSL and sole common culture medium. Eleven isolates of bacteria, includingPseudomonas, Enterobacter, Acinetobacter and Bacillus were identified as bacteria with high quorum quenching ability, especially the genus Pseudomonas showed the highest quorum quenching activity. Investigating quorum quenching mechanisms among these bacteria revealed that enzymes and non-enzymatic small compounds with quorum quenching activity were involved in this process. However, most of the bacteria showing enzymatic activity were confirmed by HPLC. Results also showed that one isolate of Acinetobacter calcoaceticus obtained by the second method, had non-enzymatic quorum quenching activity. The quorum quenching bacteria mainly reduced the biofilm and pathogenicity of Pectobacterium carotovorum subsp. carotovorum on potato and cactus. Therefore, there was a direct relationship between increasing quorum quenching activity and biocontrol ability against thispathogen. However, there were some exceptions. In addition, some isolates of Pseudomonas as the best biocontrol agents were identified, which were effective against the pathogen based on quorum quenching. | ||
| کلیدواژه‌ها [English] | ||
| acyl homoserine lactone, Acinetobacter calcoaceticus, biofilm, quorum quenching-activity, biocontrol ability | ||
| مراجع | ||
|
Adonizio, A., Kong, K. F. & Mathee, K. 2008. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrobial Agents and Chemotherapy, 52(1): 198-203. Chan, K.G., Yin, W.-F., Sam, C.K. & Koh, C.L. 2009. A novel medium for the isolation of N-acylhomoserine lactone-degrading bacteria. Journal of Industrial Microbiology & Biotechnology, 36(2): 247-251. Chen, F., Gao, Y., Chen, X., Yu, Z. & Li, X. 2013. Quorum quenching enzymes and their application in degrading signal molecules to block quorum sensing-dependent infection. International Journal of Molecular Sciences, 14(9): 17477-17500. Chatterjee, A., Cui, Y.,Hasegawa, H.,Leigh, N., Dixit, V., Chatterjee, AK. 2005. Comparative analysis of two classes of quorum-sensing signaling systems that control production of extracellular proteins and secondary metabolites in Erwinia carotovora subspecies. Journal of Bacteriology 187: 8026-38. Chong T.M., Koh C-L, Sam C.K., Choo Y.M., Yin W.F., Chan K.G. 2012.Characterization of quorum sensing and quorum quenching soil bacteria isolated from Malaysian tropical montane forest. Sensors, 12(4): 4846-59. Christiaen, S.E., Brackman, G., Nelis, H.J. & Coenye, T. 2011. Isolation and identification of quorum quenching bacteria from environmental samples. Journal of Microbiological Methods, 87(2): 213-219. Chu, Y.Y., Nega, M., Wolfle, M., Plener, L., Grond, S., Jung, K. & Gotz, F. 2013. A new class of quorum quenching molecules from Staphylococcus species affects communication and growth of Gram-negative bacteria. PLOS Pathogens, 9: 1-13. Czajkowski, R. & Jafra, S. 2009. Quenching of acyl-homoserine lactone-dependent quorum sensing by enzymatic disruption of signal molecules. Acta Biochimica Polonica, 56(1): 1-16. D’Angelo Picard C., Faure D, Penot I, Dessaux Y. 2005. Diversity of N-acyl homoserine lactone producing and degrading bacteria in soil and tobacco rhizosphere. Environmental Microbiology, 7(11):1796-808. Dong, Y.-H., Wang, L.-H. & Zhang, L.-H. 2007. Quorum-quenching microbial infections: mechanisms and implications. Philosophical Transactions of the Royal Society B: Biological Sciences, 362(1483): 1201-1211. Dong, Y.H., Xu, J.L., Li, X.Z. & Zhang, L.H. 2000. AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora. Proceedings of the National Academy of Sciences, 97(7): 3526-3531. Dong, Y.H. & Zhang, L.H. 2005. Quorum sensing and quorum-quenching enzymes. Journal of Microbiology, 43(5): 101-109. Dong, Y.H., Zhang, X.F., Xu, J.L. & Zhang, L.H. 2004. Insecticidal Bacillus thuringiensis silences Erwinia carotovora virulence by a new form of microbial antagonism, signal interference. Applied and Environmental Microbiology, 70(2): 954-960. O'Toole G. A, 2011. Microtiter dish biofilm formation assay. Journal of Visualized Experiments (47), 2437. Gould, T.A., Watson, W.T., Choi, K.H., Schweizer, H.P. & Churchill, M.E. 2004. Crystallization of Pseudomonas aeruginosa AHL synthase LasI using-turn crystal engineering. Acta Crystallographica Section D: Biological Crystallography, 60(3): 518-520. Jha, B., Kavita, K., Westphal, J., Hartmann, A. & Schmitt-Kopplin, P. 2013. Quorum sensing inhibition by Asparagopsis taxiformis, a marine macro alga: separation of the compound that interrupts bacterial communication. Marine Drugs, 11(1): 253-265. Kang, BR., Lee, JH., Ko, SJ., Lee, YH., Cha, JS., Cho, BH., Kim, YC. 2004. Degradation of acyl-homoserine lactone molecules by Acinetobacter sp. strain C1010. Canadian Journal of Microbiology, 50(11): 935-41. Kalia, V.C. 2013. Quorum sensing inhibitors: an overview. Biotechnology advances, 31(2): 224-245. LaSarre, B. & Federle, M.J. 2013. Exploiting quorum sensing to confuse bacterial pathogens. Microbiology and Molecular Bbiology Rreviews, 77(1): 73-111. Lau, Y.Y., Sulaiman, J., Chen, J.W., Yin, W.F. & Chan, K.G. 2013. Quorum sensing activity of Enterobacter asburiae isolated from lettuce leaves. Sensors, 13(10): 14189-14199. Mahmoudi, E., Ahmadi, A., Sayed-Tabatabaei, B., Ghobadi, C., Akhavan, A., Hasanzadeh, N., Venturi, V. 2011. A novel AHL-degrading rhizobacterium quenches the virulence of Pectobacterium atrosepticum on potato plant. Journal of Plant Pathology, 93 (3): 587-594. Maisuria, V.B. & Nerurkar, A.S. 2015. Interference of Quorum Sensing by Delftia sp. VM4 Depends on the Activity of a Novel N-Acylhomoserine Lactone-Acylase. PlOS one, 10(9): 138034. Molina, L., Rezzonico, F., Défago, G. & Duffy, B. 2005. Autoinduction in Erwinia amylovora: evidence of an acyl-homoserine lactone signal in the fire blight pathogen. Journal of Bacteriology, 187(9): 3206-3213. Ni, N., Li, M., Wang, J. & Wang, B. 2009. Inhibitors and antagonists of bacterial quorum sensing. Medicinal Research Reviews, 29(1): 65-124. Ochiai, S., Yasumoto, S., Morohoshi, T., Ikeda, T. 2014. AmiE, a Novel N-Acylhomoserine Lactone Acylase Belonging to the Amidase Family, from the Activated-Sludge Isolate Acinetobacter sp. Strain Ooi24. Applied and Environmental Microbiology, 80(22): 6919-25. Qian, G.L., Fan, J.Q., Chen, D.F., Kang, Y.J., Han, B., Hu, B.S. & Liu, F.Q. 2010. Reducing Pectobacterium virulence by expression of an N-acyl homoserine lactonase gene Plpp-aiiA in Lysobacter enzymogenes strain OH11. Biological Control, 52(1): 17-23. Otto, M. 2004. Quorum‐sensing control in Staphylococci–a target for antimicrobial drug therapy. FEMS Microbiology Letters, 241(2): 135-141. Põllumaa, L., Alamäe, T. & Mäe, A. 2012. Quorum sensing and expression of virulence in pectobacteria. Sensors, 12(3): 3327-3349. Rajesh, P., Rai, VR. 2014.Molecular identification of aiiA homologous gene from endophytic Enterobacter species and in silico analysis of putative tertiary structure of AHL-lactonase. Biochemical and Biophysical Research Communications, 443(1): 290-5. Rasmussen, T.B. & Givskov, M. 2006. Quorum sensing inhibitors: a bargain of effects. Microbiology, 152(4): 895-904. Romero, M., Diggle, S.P., Heeb, S., Cámara, M. & Otero, A. 2008. Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase. FEMS Microbiology Letters, 280(1): 73-80. Saha, N, Chaudhary, A.,Singh, S., Singh, D., Walia, S., Das, T. 2015. Plant Pathogenic Microbial Communication Affected by Elevated Temperature in Pectobacterium carotovorum subsp. carotovorum. Current Microbiology, 71: 585-93. Sammer, UF., Völksch B, Möllmann U, Schmidtke M, Spiteller P, Spiteller M, et al. 2009. 2-Amino-3-(oxirane-2, 3-dicarboxamido)-propanoyl-valine, an effective peptide antibiotic from the epiphyte Pantoea agglomerans 48b90. Applied and Environmental Microbiology, 75(24): 7710-7717. Sio, CF., Otten, LG., Cool, RH., Diggle, SP., Braun, PG., Bos, R., et al. 2006. Quorum quenching by an N-acyl-homoserine lactone acylase from Pseudomonas aeruginosa PAO1. Infection and Immunity, 74(3): 1673-82. Teasdale, M.E., Liu, J., Wallace, J., Akhlaghi, F. & Rowley, D.C. 2009. Secondary metabolites produced by the marine bacterium Halobacillus salinus that inhibit quorum sensing-controlled phenotypes in gram-negative bacteria. Applied and Environmental Microbiology, 75(3): 567-572. Thomas, P.W., Stone, E.M., Costello, A.L., Tierney, D.L. & Fast, W. 2005. The quorum-quenching lactonase from Bacillus thuringiensis is a metalloprotein. Biochemistry, 44(20): 7559-7569. Uroz, S., Chhabra, S.R., Cámara, M., Williams, P., Oger, P. & Dessaux, Y. 2005. N-Acylhomoserine lactone quorum-sensing molecules are modified and degraded by Rhodococcus erythropolis W2 by both amidolytic and novel oxidoreductase activities. Microbiology, 151(10): 3313-3322. Uroz, S., Oger, P., Chhabra, S.R., Cámara, M., Williams, P. & Dessaux, Y. 2007. N-acyl homoserine lactones are degraded via an amidolytic activity in Comamonas sp. strain D1. Archives of Microbiology, 187(3): 249-256. Von Bodman, S.B., Bauer, W.D. & Coplin, D.L. 2003. Quorum sensing in plant-pathogenic bacteria. Annual Review of Phytopathology, 41(1): 455-482. Yu SM., Lee YH. 2015.Genes involved in nutrient competition by Pseudomonas putida JBC17 to suppress green mold in postharvest satsuma mandarin. Journal of Basic Microbiology,(55): 898–906. | ||
|
آمار تعداد مشاهده مقاله: 625 تعداد دریافت فایل اصل مقاله: 569 |
||