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جداسازی باکتری‌های دارای خاصیت quorum quenching با قابلیت بیوکنترل Pectobacterium carotovorum subsp. carotovorum | ||
| مهار زیستی در گیاهپزشکی | ||
| مقاله 3، دوره 4، شماره 2 - شماره پیاپی 8، اسفند 1395، صفحه 25-38 اصل مقاله (265.74 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2017.112889 | ||
| نویسندگان | ||
| محمد رضا عالی منش1؛ پریسا طاهری* 2؛ سعید طریقی3 | ||
| 1گروه بیماری شناسی گیاهی دانشگاه فردوسی مشهد | ||
| 2دانشیار/ گروه گیاه پزشکی دانشکده کشاورزی دانشگاه فردوسی مشهد | ||
| 3گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد | ||
| چکیده | ||
| تعداد 177 جدایه باکتری کونچر به دو روش استفاده از محیط کشت حداقلی حاوی سیگنال آسیل همو سرین لاکتون از نوع 3-oxo-C6-HSL و محیط کشت‌های عمومی از گیاهان تیره‌ی سولاناسه جداسازی شدند. از بین آنها 11 جدایه متعلق به جنس‌های Pseudomonas، Enterobacter،Acinetobacterو Bacillus فعالیت کونچری بالایی داشتند. بهویژه جنس Pseudomonas که دارای بالاترین میزان فعالیت بود. بررسی سازوکار کونچری در این باکتری‌ها نشان داد که آنزیم‌ها و ترکیبات کوچک غیرآنزیمی با فعالیت کونچری در این فرایند نقش دارند. بیشتر این باکتری‌ها فقط دارای فعالیت آنزیمی بودندکه توسط آزمون HPLC تأیید شدند. همچنین برای اولین‌بار جدایه‌ای از باکتری calcoaceticus Acinetobacterکه با روش دوم جداسازی شده بود با توانایی کونچری غیرآنزیمی شناسایی شد. باکتری‌های کونچر عمدتاً باعث کاهش بیوفیلم و کاهش بیماری‌زایی Pectobacterium carotovorum subsp. carotovorumروی سیب‌زمینی و کاکتوس شدند. بنابراین، رابطه‌ی مستقیمی بین افزایش فعالیت کونچری و توانایی بیوکنترلی علیه این بیمارگر وجود داشت، هر چند موارد استثناء نیز مشاهده شد. همچنین، برخی جدایه‌های Pseudomonasبه‌عنوان بهترین عوامل بیوکنترل که براساس فعالیت کونچری علیه بیمارگر مذکور عمل می‌کردند، تشخیص داده شدند. | ||
| کلیدواژه‌ها | ||
| آسیل‌همو‌سرین لاکتون؛ Acinetobacter calcoaceticus؛ بیوفیلم؛ فعالیت کونچری؛ توانایی بیوکنترلی | ||
| عنوان مقاله [English] | ||
| Isolation of the bacteria with quorum quenching activity against Pectobacterium carotovorum subsp. carotovorum | ||
| نویسندگان [English] | ||
| Mohammad Reza Alymanesh1؛ Parissa Taheri2؛ Saeed Tarighi3 | ||
| 2Associate professor/Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad | ||
| چکیده [English] | ||
| One hundred seventhy seven quorum quenching bacteria were isolated from Solanaceae family using two methods of minimal medium containing synthetic acyl homoserine lactone signal 3-oxo-C6-HSL and sole common culture medium. Eleven isolates of bacteria, includingPseudomonas, Enterobacter, Acinetobacter and Bacillus were identified as bacteria with high quorum quenching ability, especially the genus Pseudomonas showed the highest quorum quenching activity. Investigating quorum quenching mechanisms among these bacteria revealed that enzymes and non-enzymatic small compounds with quorum quenching activity were involved in this process. However, most of the bacteria showing enzymatic activity were confirmed by HPLC. Results also showed that one isolate of Acinetobacter calcoaceticus obtained by the second method, had non-enzymatic quorum quenching activity. The quorum quenching bacteria mainly reduced the biofilm and pathogenicity of Pectobacterium carotovorum subsp. carotovorum on potato and cactus. Therefore, there was a direct relationship between increasing quorum quenching activity and biocontrol ability against thispathogen. However, there were some exceptions. In addition, some isolates of Pseudomonas as the best biocontrol agents were identified, which were effective against the pathogen based on quorum quenching. | ||
| کلیدواژه‌ها [English] | ||
| acyl homoserine lactone, Acinetobacter calcoaceticus, biofilm, quorum quenching-activity, biocontrol ability | ||
| مراجع | ||
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