| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,846 |
| تعداد مقالات | 32,300 |
| تعداد مشاهده مقاله | 41,387,189 |
| تعداد دریافت فایل اصل مقاله | 18,562,197 |
Molecular and Microscopic Detection of Theileria spp. among Cattle and Buffaloes in West Azarbaijan, Iran | ||
| Archives of Razi Institute | ||
| مقاله 6، دوره 72، شماره 3، آذر 2017، صفحه 189-195 اصل مقاله (474.6 K) | ||
| نوع مقاله: Short Communication | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2017.111605 | ||
| نویسندگان | ||
| B. Narimani1؛ N. Hoghooghi-Rad* 2؛ P. Shayan3؛ S. Rahbari4 | ||
| 1Department of Pathobiology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Department of Parasitology and Mycology, Faculty of Specialized Veterinary Science, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 3Department of Pathobiology, Faculty of Veterinary Science, Tehran University, Tehran, Iran | ||
| 4Department of Microbiology, Faculty of Specialized Veterinary Science, Branch of Science and Research, Islamic Azad University, Tehran, Iran | ||
| چکیده | ||
| Bovine theileriosis is an important tick-borne disease caused by intraerythrocytic parasites from genus Theileria. This study sought to detect the theileriosis among cattle and buffaloes using molecular and microscopic tests in West Azerbaijan, Iran. For this purpose, 484 blood samples from 193 cattle and 291 buffaloes were collected during March to July 2014. The breed, gender, age, and habitat of these animals were recorded. These animals were native and apparently healthy, living in four different cities of the province. The blood films were stained with Giemsa’s for microscopic examinations. Direct cell semi-nested polymerase chain reaction (PCR) assay was performed to detect T.annulata DNA with Tbs-S/Tbs-A and To-S/Tbs-A primer pairs targeted to 18S ribosomal RNA gene for Theileria spp. and T.orientalis amplification, respectively. The molecular assays revealed that 36 cattle (18.65%) were infected, in which 15 cattle were infected by both T.annulata and T.orientalis. Out of 291 buffaloes, four samples (1.4%) were infected by Theileria genotypes, and two buffaloes (0.7%) were infected only by T.orientalis. The observational results of the gender, age, and habitat of the studied animals were similar to animals of the other parts of Iran. The present study indicated that T.orientalis may be prevalent in native cattle and buffaloes throughout the northern parts of Iran. This study assessed the infection of buffaloes with T.orientalis for the first time. | ||
| کلیدواژه‌ها | ||
| Buffalo؛ Cattle؛ Iran؛ PCR؛ Theileria orientalis | ||
| عنوان مقاله [English] | ||
| تشخیص مولکولی و میکروسکوپی تیلریا اورینتالیس در گاوهای استان آذربایجان غربی، ایران | ||
| چکیده [English] | ||
| تیلریوز گاوی، بیماری کنه برد مهمی است که توسط تک سلولی داخل اریتروسیتی از جنس تیلریا ایجاد میگردد. این بررسی برای تعیین وضعیت جاری عفونت تیلریایی در گاوهای استان آذربایجان غربی ایران توسط روش های مولکولی و میکروسکوپی به کار برده شد. در این تحقیق، 484 نمونه خون کامل از گاو بومی و گاومیش چهار شهر در طی مارس تا جولای 2014 جمع آوری شده است. دامهای مورد مطالعه بر اساس نژاد، جنس، سن، ماه و شهر طبقه بندی شده بودند. تمام گسترشهای نازک خونی از هر دام، توسط رنگ گیمسا برای مطالعه انگل شناسی رنگ آمیزی شدند. تکنیکهای واکنش زنجیرهای پلیمراز و Semi-nested PCR آزمونهای دیگری بودند که برای تشخیص DNA ی تیلریا اورینتالیس، جفت 18S rRNA ویژه به کار گرفته شدند: مجموعه پرایمری Tbs-S/Tbs-A برای تکثیر تیلریایی و سپس مجموعه پرایمری To/Tbs-A برای تکثیر تیلریا اورینتالیس. تکنیک مولکولی، 40 عدد از 484 (26/8 %) نمونه را از لحاظ جنس تیلریا مثبت و 17 عدد از 484 (51/3 %) نمونه را از لحاظ تیلریا اورینتالیس مثبت، آشکار کرد. میزان شیوع تیلریا اورینتالیس در گاوهای بومی 33/8 % و برای نخستین بار در گاومیش ها 68/0 % بوده و بین گروههای گاوبومی و گاومیش اختلاف معنی دار مشاهده گردید. (P<0.05) این تکنیک مولکولی اذعان داشت که تیلریا اورینتالیس در گاو بومی و به خصوص گاومیش در مناطق شمال غربی ایران وجود دارد. | ||
| کلیدواژه‌ها [English] | ||
| تیلریا اورینتالیس, گاومیش, گاو, واکنش زنجیره ای پلیمراز, ایران | ||
| مراجع | ||
|
Ahmed, J.S., Luo, J., Schnittger, L., Seitzer, U., Jongejan, F., Yin, H., 2006. Phylogenetic position of small-ruminant infecting piroplasms. Ann N Y Acad Sci 1081, 498-504.
Akbari, j., Javanbakht, J., Tavassoli, M., Tabatabai, M., Shafiei, R., 2012. Molecular survey of Theileria annulata in cattle by PCR-RFLP method in Iran. Bacteriol Parasitol 3, 1-6.
Aktas, M., Altay, K., Dumanli, N., 2006. A molecular survey of bovine Theileria parasites among apparently healthy cattle and with a note on the distribution of ticks in eastern Turkey. Vet Parasitol 138, 179-185.
Almeria, S., Castellà, J., Ferrer, D., Ortuño, A., Estrada-Peña, A., Gutiérrez, J.F., 2001. Bovine piroplasms in Minorca (Balearic Islands, Spain): a comparison of PCR-based and light microscopy detection. Veterinary Parasitology 99, 249-259.
Altay, K., Aydin, M.F., Dumanli, N., Aktas, M., 2008. Molecular detection of Theileria and Babesia infections in cattle. Vet Parasitol 158, 295-301.
Dumanli, N., Aktas, M., Cetinkaya, B., Cakmak, A., Koroglu, E., Saki, C.E., et al., 2005. Prevalence and distribution of tropical theileriosis in eastern Turkey. Vet Parasitol 127, 9-15.
Garcı́a-Garcı́a, J.C., Montero, C., Redondo, M., Vargas, M., Canales, M., Boue, O., et al., 2000. Control of ticks resistant to immunization with Bm86 in cattle vaccinated with the recombinant antigen Bm95 isolated from the cattle tick, Boophilus microplus. Vaccine 18, 2275-2287.
Ghaemi, P., Hoghooghi-Rad, N., Shayan, P., Eckert, B., 2012. Detection of Theileria orientalis in Iran by semi-nested PCR. Parasitol Res 110, 527-531.
Hashemi-Fesharki, R., 1988. Control of Theileria annulata in Iran. Parasitology Today 4, 36-40.
Hashemi-Fesharki, R., Habibi, G.R., Ahourai, P., 1998. Delayed type hypersensitivity theilerin test in cattle vaccinated against Theileria annulata infection. Veterinary Parasitology 75, 261-263.
Hayashida, K., Hara, Y., Abe, T., Yamasaki, C., Toyoda, A., Kosuge, T., et al., 2012. Comparative genome analysis of three eukaryotic parasites with differing abilities to transform leukocytes reveals key mediators of Theileria-induced leukocyte transformation. MBio 3, e00204-00212.
Hoghooghi-Rad, N., Ghaemi, P., Shayan, P., Eckert, B., Sadr-Shirazi, N., 2011. Detection of native carrier cattle infected with Theileria annulata by semi-nested PCR in Golestan province of Iran. World Appl Sci J 12 317-323.
Mehlhorn, H., 2008. Encyclopedia of Parasitology: A-M, Springer.
Noaman, V., 2013. A molecular study on Theileria and Babesia in cattle from Isfahan province, Central Iran. J Parasit Dis 37, 208-210.
Ota, N., Mizuno, D., Kuboki, N., Igarashi, I., Nakamura, Y., Yamashina, H., et al., 2009. Epidemiological survey of Theileria orientalis infection in grazing cattle in the eastern part of Hokkaido, Japan. J Vet Med Sci 71, 937-944.
Rahbari, S., Nabian, S., Shayan, P., 2007. Primary report on distribution of tick fauna in Iran. Parasitol Res 101 Suppl 2, S175-177.
Rodriguez-Valle, M., Taoufik, A., Valdes, M., Montero, C., Ibrahin, H., Hassan, S.M., et al., 2012. Efficacy of Rhipicephalus (Boophilus) microplus Bm86 against Hyalomma dromedarii and Amblyomma cajennense tick infestations in camels and cattle. Vaccine 30, 3453-3458.
Safarpour Dehkordi, F., Parsaei, P., Saberian, S., Moshkelani, S., Hajshafiei, P., Hoseini, S.R., 2012. Prevalence study of T.annulata by comparison of four diagnostic techniques in South-west Iran. Bulg J Vet 2, 123-130.
Savini, G., Onuma, M., Scaramozzino, P., Kakuda, T., Semproni, G., Langella, V., 1998. First report of Theileria sergenti and T. buffeli/orientalis in cattle in Italy. Ann N Y Acad Sci 849, 404-407.
Shayan, P., Jafari, S., Fattahi, R., Ebrahimzade, E., Amininia, N., Changizi, E., 2016. Identification and characterization of Theileria ovis surface protein (ToSp) resembled TaSp in Theileria annulata. Parasitol Res 115, 1893-1899.
Shayan, P., Rahbari, S., 2005. Simultaneous differentiation between Theileria spp. and Babesia spp. on stained blood smear using PCR. Parasitol Res 97, 281-286.
Uilenberg, G., 1981. Theilerial Species of Domestic Livestock. In: Irvin, A.D., Cunningham, M.P., Young, A.S. (Eds.), Advances in the Control of Theileriosis: Proceedings of an International Conference held at the International Laboratory for Research on Animal Diseases in Nairobi, 9–13th February, 1981, Springer Netherlands, Dordrecht, pp. 4-37.
Uilenberg, G., Hashemi-Fesharki, R., 1984. Theileria orientalis in Iran. Vet Q 6, 1-4.
Willadsen, P., Smith, D.O.N., Cobon, G., McKenna, R.V., 1996. Comparative vaccination of cattle against Boophilus microplus with recombinant antigen Bm86 alone or in combination with recombinant Bm91. Parasite Immunology 8, 241-246.
Yokoyama, N., Ueno, A., Mizuno, D., Kuboki, N., Khukhuu, A., Igarashi, I., et al., 2011. Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa prefectures of Japan. J Vet Med Sci 73, 305-312.
| ||
|
آمار تعداد مشاهده مقاله: 1,706 تعداد دریافت فایل اصل مقاله: 2,842 |
||