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القاء کالوس و تولید اسیدهای فنولیک درکشت کالوس مریستم انتهایی و رویان گیاه نوروزک (Salvia leriifolia Benth.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 12، دوره 33، شماره 2 - شماره پیاپی 82، تیر 1396، صفحه 304-313 اصل مقاله (209.18 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2017.105632.1735 | ||
| نویسنده | ||
| معصومه مدرس* | ||
| استادیار، گروه زیست‌شناسی، دانشگاه فرهنگیان، مشهد، ایران | ||
| چکیده | ||
| نوروزک (Salvia leriifolia Benth.) از خانواده نعناعیان، دارای خواص ضددرد، ضدالتهاب، ضددیابت و آنتی‌اکسیدان است. به‌منظور القاء کالوس از مریستم انتهایی و رویان گیاه نوروزک برای تولید اسیدهای فنولیک آزمایشی در قالب طرح کاملاً تصادفی اجرا شد. جداکشت‌های مریستم انتهایی و رویان بر روی محیط کشت MS همراه با ترکیب‌های مختلف از مواد تنظیم‌کننده رشد 2,4-D (صفر، 1، 2 و 3 میلی‌گرم بر لیتر) با KIN (صفر، 3/0 و 1 میلی‌گرم بر لیتر) و نیز BAP (صفر، 0.5، 1، 2 و 3 میلی‌گرم بر لیتر) با NAA (صفر، 0.5، 1 و 1.5 میلی‌گرم بر لیتر) کشت داده شد. پس از چهار هفته درصد تولید کالوس، وزن تر و خشک کالوس‌ها بررسی گردید و تجمع اسیدهای فنولیک با استفاده از تکنیک کروماتوگرافی مایع با کارایی بالا (HPLC) اندازه‌گیری شد. بهترین تیمار برای القاء و رشد کالوس از مریستم انتهایی و رویان 2,4-D، 2 میلی‌گرم بر لیتر با Kin 1 میلی‌گرم بر لیتر بود. میزان تجمع کافئیک اسید در کالوس‌های رویان در این تیمار به‌طور معنی‌داری بیشتر از برگ گیاه بود و میزان رزمارینیک اسید تولید شده قابل مقایسه با برگ بود. بیشترین میزان سالویانولیک اسیدB از عصاره حاصل از کالوس مریستم انتهایی بدست آمد که حدود سه برابر بیشتر از برگ گیاه نوروزک بود. | ||
| کلیدواژه‌ها | ||
| کافئیک اسید؛ رزمارینیک اسید؛ سالویانولیک اسیدB؛ کالوس | ||
| عنوان مقاله [English] | ||
| Callus induction and phenolic acids production in apical meristem and embryo callus culture of Salvia leriifolia Benth. | ||
| نویسندگان [English] | ||
| M. Modarres | ||
| Department of Biology, Farhangian University, Tehran, Iran | ||
| چکیده [English] | ||
| Salvia leriifolia Benth., belonging to Lamiaceae, has antinociceptive, anti-inflammatory, antidiabetic and antioxidant properties. In order to callus induction from apical meristem and embryo of salvia leriifolia to produce phenolic acids, an experiment was carried out in a completely randomized design. The apical meristem and embryo explants were cultured in MS medium supplemented with 2,4-D (0, 1, 2, 3mg/L), KIN (0, 0.3, 1mg/L), BAP (0, 0.5, 1, 2, 3mg/L) and NAA (0, 0.5, 1, 1.5mg/L). The fresh and dry weights were evaluated and accumulation of phenolic acids was measured after four weeks of culture by HPLC. The best treatments for callus induction and growth was at the 2,4-D 2mg/L and KIN 1mg/L. Accumulation of caffeic acid in embryo callus was higher than that of leaves of Salvia leriifolias and concentration of rosmarinic acid was comparable to the leaves. The highest salvianolic acid B concentration was found in callus of apical meristem that was about four times higher than that of leaves. | ||
| کلیدواژه‌ها [English] | ||
| caffeic acid, rosmarinic acid, salvianolic acid B, Callus | ||
| مراجع | ||
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