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تأثیر تنش خشکی و جاسمونیک اسید روی صفات مورفولوژیکی و فیتوشیمیایی نعناع فلفلی (Mentha piperita L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 9، دوره 33، شماره 2 - شماره پیاپی 82، تیر 1396، صفحه 268-280 اصل مقاله (264.18 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2017.106481.1783 | ||
| نویسندگان | ||
| عزیزاله خیری* 1؛ هانیه توری2؛ نجم الدین مرتضوی3 | ||
| 1استادیار، بخش گیاهان دارویی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، ایران | ||
| 2دانش‌آموخته کارشناسی ارشد، بخش گیاهان دارویی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، ایران | ||
| 3استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، ایران | ||
| چکیده | ||
| نعناع فلفلی با نام علمی Mentha piperita L. از خانواده نعناعیان، یکی از گیاهان دارویی پرمصرف است که علاوه‌بر آثار درمانی به‌عنوان طعم‌دهنده در تولید محصولات غذایی و دارویی مختلف بکار می‌رود. کمّیت و کیفیت مواد مؤثره گیاهان دارویی علاوه‌بر ژنوتیپ، تحت تأثیر عوامل محیطی نیز قرار می‌گیرد. از این‌رو به‌منظور بررسی اثرات تنش خشکی و جاسمونیک اسید بر روی کمّیت و کیفیت نعناع فلفلی، آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی در سال 1394 در دانشگاه زنجان اجرا شد. سطوح آبیاری در سه سطح (50، 75 و 100 (شاهد) درصد ظرفیت زراعی) به‌عنوان فاکتور اصلی و غلظت جاسمونیک اسید در سه سطح (صفر، 50 و 100 میلی‌گرم بر لیتر) به‌عنوان فاکتور فرعی در نظر گرفته شد. برای تکثیر از ریزوم‌های گیاه استفاده گردید. نمونه‌ها در مرحله گلدهی جمع‌آوری و پس از خشک شدن در سایه، به روش تقطیر با آب، اسانس گیاه استخراج شد و شناسایی ترکیب‌های اسانس به کمک دستگاه GC و GC/MS انجام گردید. نتایج نشان داد که بیشترین میزان ارتفاع، وزن تر و خشک، کلروفیل کل و محتوای نسبی آب برگ در تیمار شاهد با کاربرد 100 میلی‌گرم بر لیتر جاسمونیک اسید بدست آمد. همچنین بیشترین میزان آنزیم پراکسیداز در تنش خشکی 50% ظرفیت زراعی با کاربرد 100 میلی‌گرم بر لیتر جاسمونیک اسید حاصل شد. همچنین بیشترین درصد اسانس در تنش خشکی 75% ظرفیت زراعی با کاربرد 50 میلی‌گرم بر لیتر جاسمونیک اسید بدست آمد. ترکیب‌های اصلی شناسایی شده اسانس شامل منتول، منتون، 8،1-سینئول، سیکلوهگزانول و متیل استات بودند که بیشترین مقادیر منتول، منتون و 8،1-سینئول در تیمار 100 میلی‌گرم بر لیتر جاسمونیک اسید و بیشترین مقدار سیکلوهگزانول در تیمار 50 میلی‌گرم بر لیتر جاسمونیک اسید بدست آمد. اما با کاربرد جاسمونیک اسید میزان متیل استات از 7.24% در تیمار شاهد به 0.56% در تیمار 100 میلی‌گرم بر لیتر کاهش یافت. | ||
| کلیدواژه‌ها | ||
| الیسیتور؛ کم آبیاری؛ کروماتوگرافی گازی؛ درصد اسانس؛ نعناع فلفلی (Mentha piperita L.) | ||
| عنوان مقاله [English] | ||
| Effects of drought stress and jasmonic acid elicitors on morphological and phytochemical characteristics of peppermint (Mentha piperita L.) | ||
| نویسندگان [English] | ||
| A. Kheiry1؛ H. Tori2؛ N. Mortazavi3 | ||
| 1Medicinal Plants Section, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran, | ||
| 2Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran | ||
| 3Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran | ||
| چکیده [English] | ||
| Peppermint (Mentha piperita L.) from Lamiaceae family is one of the most widely used medicinal plants. In addition to therapeutic effects, it is used as flavoring in the production of various foods and medicinal products. In addition to genotype, the quality and quantity of active ingredients in medicinal plants are influenced by environmental factors. This research was aimed to study the effects of drought stress and jasmonic acid on the quality and quantity of peppermint as a factorial experiment in a completely randomized design at University of Zanjan in 2015. The treatments consisted of three irrigation levels [50%, 75%, and 100% (control) of field capacity] as main factor and three concentrations of jasmonic acid (0, 50, and 100 mg l-1) as sub-factor. Rhizomes of plants were used for plant production. The plant samples were collected at flowering stage and after drying in the shade, the essential oil was extracted with water distillation method by Clevenger apparatus. The essential oil components were identified by GC and GC-MS. The results showed that the highest fresh and dry weight, height, total chlorophyll and relative leaf water content were obtained in control with 100 mg l-1 jasmonic acid. The highest peroxidase enzyme content was obtained at 50% field capacity drought stress with 100 mg l-1 jasmonic acid. The highest essential oil content was obtained at 75% field capacity drought stress with application of 50 mg l-1 jasmonic acid. The main essential oil components were menthol, menthon, 1,8-cineole, cyclohexanol, and methyl acetate. The highest amounts of menthol, menton, and 1,8-cineole were obtained from 100 mg l-1 jasmonic acid and the highest amount of cyclohexanol was obtained from 50 mg l-1 jasmonic acid. However, application of jasmonic acid decreased the amount of methyl acetate from 7.24% in control to 0.56% in 100 mg l-1 of JA treatment. | ||
| کلیدواژه‌ها [English] | ||
| Elicitor, essential oil, less irrigation, Gas chromatography, Mentha piperita L | ||
| مراجع | ||
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