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بررسی ترکیب‌های شیمیایی اسانس گیاه Pimpinella olivierioides Boiss. & Hausskn. | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 2، دوره 33، شماره 2 - شماره پیاپی 82، تیر 1396، صفحه 185-195 اصل مقاله (216.09 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2017.104986.1686 | ||
| نویسندگان | ||
| فاطمه عسکری* 1؛ شهلا احمدی2 | ||
| 1استادیار، مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 2مربی پژوهشی، مرکز تحقیقات کشاورزی و منابع طبیعی استان لرستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، خرم‌آباد، ایران | ||
| چکیده | ||
| جنس Pimpinella با حدود 180-170 گونه در جهان، یکی از بزرگترین جنس‌های خانواده چتریان می‌باشد. براساس مطالعات فلور ایران، این جنس در فلات ایران، 25 گونه دارد که 22 گونه آن در ایران است و 6 گونه از آنها بومی هستند. گونه Pimpinella olivierioides Boiss. & Hausskn. در منطقه غرب و مرکز پراکنش دارد. هدف از انجام این تحقیق، تعیین ترکیب‌های شیمیایی موجود در اسانس گونه P. olivierioides می‌باشد. اندام‌های گیاه P. olivieroides در مرحله گلدهی در اواخر تیر 1391 از منطقه نهاوند جمع‌آوری و پس از تفکیک در دمای محیط خشک و بعد به ذرات کوچک آسیاب شدند. نمونه ساقه و برگ، ریشه و گل‌آذین به تفکیک با روش تقطیر با آب اسانس‌گیری شدند. برای جداسازی و شناسایی ترکیب‌های اسانس از دستگاه‌های گاز کروماتوگرافی GC و گاز کروماتوگرافی متصل به طیف‌سنج جرمی (GC/MS) استفاده شد. بازده اسانس ساقه و برگ گیاه P. olivieroides (بر پایه وزنی-وزنی خشک شده) 0.09%، ریشه 0.52% و گل‌آذین 0.57% بود. بیشترین درصد ترکیب‌های اسانس ساقه و برگ، ریشه و گل‌آذین به‌ترتیب جرماکرن D (36.5%، 21.9% و 11.1%) و بی‌سیکلوجرماکرن (7.5%، 15.9% و 4.4%) بودند. بتا-بیزابولن (24.9%) فقط در اسانس ریشه و ترانس-پینوکامفن (14.7%) و سیس-پینوکامفن (13.4%) دیگر ترکیب‌های شاخص اسانس ساقه و برگ بودند. نکته جالب توجه در مورد این اسانس‌ها، رنگ آنها بود که به‌ترتیب آبی روشن، آبی کاربنی و سبز چمنی بود. با مقایسه ترکیب‌های اصلی اسانس مشخص می‌شود که تنوع ترکیب‌ها در اسانس اندام‌های مختلف وجود دارد. | ||
| کلیدواژه‌ها | ||
| Pimpinella olivierioides Boiss. & Hausskn؛ Umbelliferae؛ ترکیب‌های شیمیایی اسانس؛ جرماکرن D؛ بتا-بیزابولن | ||
| عنوان مقاله [English] | ||
| Study of chemical composition of the Pimpinella olivierioides Boiss. & Hausskn. essential oils from Iran | ||
| نویسندگان [English] | ||
| F. Askari1؛ Sh. Ahmadi2 | ||
| 1Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| 2Lorestan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Khorramabad, Iran | ||
| چکیده [English] | ||
| Pimpinella,with about 170-180 species in the world, is one of the largest genus of Apiaceae. Based on the flora of Iran, there are 25 species of Pimpinella in Iranian plateau, so that 22 species with six endemic species are found in Iran. P. olivierioides Boiss. & Hausskn. is distributed in West and Central Iran. The aim of this study was to determine chemical composition of P. olivierioides essential oil. The plant parts of P. olivieroides were collected at flowering stage from Nahavand (Hamedan Province), on July 2012. The plant organs including stem and leaf and inflorescence were dried in laboratory and crushed to particles. The essential oils were obtained by hydrodistillation and analyzed by GC and GC/MS. The yield of stem and leaf essential oils (w/w dried weight) was 0.09%, root 0.52% and inflorescence 0.57%. The major components of stem and leaf, root and inflorescence essential oils were Germacrene D (36.5%, 21.9% and 11.1%), Bicyclogermacrene (7.5%, 15.9% and 4.4%), respectively. b-bisabolene (24.9%) was found only in root essential oil. Trans-pinocamphene (14.7%) and cis-pinocamphene (13.4%) were found only in stem and leaf essential oils. The interesting thing about the essential oils was their colors. The color of stem and leaf, root and inflorescence essential oils was light blue, blue, and green grass, respectively. Our results clearly showed that very different compositions were found in the essential oil of P. olivierioides organs. | ||
| کلیدواژه‌ها [English] | ||
| Pimpinella olivierioides Boiss. & Hausskn, Umbelliferae, chemical composition of essential oil, germacrene D, b-Bisabolene | ||
| مراجع | ||
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