To identify races of T. laevis, 20 infected samples of wheat were collected from important wheat grown areas. Seeds of the differential monogenic and oligogenic lines were artificially infected with teliospores of different samples and grown in the field. At harvesting time, percentage of infected spikes was assessed for each line, and races of each sample were determined, using standard method for race identification. According to the results, five isolates being virulent on Bt7 was determined as race L-1, four isolates being virulent on Ba and Bt? as race L-3, two isolates being virulent on Btl and Bt? as race L-4, eight isolates being virulent on Bt2, Bt3 and Bt7 as race L-1 0 and one isolate being virulent on Bt2, Bt3, Bt4, Bt6 and Bt7 as race L-17. Genes Bt2, Bt3 and Bt7 were susceptible to most of the isolates, therefore these genes are not recommended for using in breeding programs, Bt8, Bt9 and Btl3 were susceptible to some isolates. Genes Bt1, Bt4, Bt6 (except one race), Bt5, Bt10, Bt11, Bt12, Bt14 and BtP were resistant to all identified races, these genes are effective for development of resistant cultivars in different parts of the Iran. |