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تجزیه بیان ژنهای بیوسنتزی تیمول و کارواکرول در بافتهای مختلف آویشن باغی (Thymus vulgaris) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 13، دوره 25، شماره 1 - شماره پیاپی 49، خرداد 1396، صفحه 160-171 اصل مقاله (297.91 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.109829 | ||
| نویسندگان | ||
| عاطفه مشهدی ملکزاده1؛ محمد مجدی* 2؛ اسعد معروفی3 | ||
| 1کارشناسی ارشد بیوتکنولوژی کشاورزی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه کردستان، سنندج | ||
| 2نویسنده مسئول مکاتبات، استادیار، دانشکده کشاورزی، دانشگاه کردستان، سنندج | ||
| 3استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه کردستان، سنندج | ||
| چکیده | ||
| آویشن باغی (Thymus vulgaris) یکی از مهمترین گیاهان دارویی است که بیش از 20 نوع ترکیب شیمیایی مهم شناخته شده دارد. ژن 1-دی اکسی دی زایلوز 5-فسفات ردوکتوایزومراز (DXR) بهعنوان نقطه کنترلی مهم در مسیر MEP عمل میکند، زیرا اولین مرحله اصلی و متمایزکننده مسیر MEP میباشد. گاماترپینن جزء کلیدی اسانس در آویشن باغی میباشد، که حدود 30% از اسانس را تشکیل میدهد و بهوسیله گاماترپینن سنتاز تولید میشود. سیتوکرومهای مختلف از P450s شامل CYP71D178 و CYP71D180 است که با واسطه گاماترپینن در بیوسنتز تیمول و کارواکرول دخیل هستند. این پژوهش، با هدف بررسی الگوی بیان ژنهای دخیل در بیوسنتز تیمول و کارواکرول شامل DXR، گاماترپینن سنتاز، CYP71D178وCYP71D180در بافتهای گل و برگ آویشن باغی انجام شد. در این مطالعه الگوی بیان این ژنهادر بافتهای گل و برگ با استفاده از تکنیک RT-PCR نیمهکمیبررسی شد. میزان بیان ژن در بافت گل نسبت به برگ برای ژنهای DXR، گاماترپینن سنتاز، CYP71D178 و CYP71D180 بهترتیب 7/1، 7/2، 9/1 و 2 برابر بیشتر بود و شدت بیان ژنهای مختلف نیز با هم متفاوت بود. میزان بیان این ژنها در کرکهای غدهای بالا بود. بنابراین بیان بالا در بافت گل احتمالاً بهدلیل تراکم بالای کرکهای غدهای میباشد. با توجه به این نتایج میزان اسانس بیشتر در گل نسبت به برگ میتواند ناشی از بیان بیشتر ژنهای بررسی شده در گل نسبت به برگ باشد. | ||
| کلیدواژهها | ||
| DXR؛ آویشن؛ گاماترپینن؛ CYP71D178؛ CYP71D180؛ بیان ژن | ||
| عنوان مقاله [English] | ||
| Expression analysis of biosynthetic genes of thymol and carvacrol in different tissues of thyme (Thymus vulgaris) | ||
| نویسندگان [English] | ||
| A. Mashhady Malekzadeh1؛ M. Majdi2؛ A. Maroufi3 | ||
| 1Sc., Department of Agriculture, College of Agriculture, University of Kurdistan, Sanandaj, I.R. Iran | ||
| 2Assist. Prof., College of Agriculture, University of Kurdistan, Sanandaj, I.R. Iran. | ||
| 3Assist. Prof., College of Agriculture, University of Kurdistan, Sanandaj, I.R. Iran. | ||
| چکیده [English] | ||
| Thymus vulgaris is one of the most important medicinal plants with more than 20 types of known important chemical compositions. A gene named 1-deoxy-d-xylulose 5-phosphate reductoisomerase (DXR) acts as an important control point, as this is the first committed step in the MEP pathway. γ-terpinene is a key component in essential oil of thyme which constituted up to 30% of the essential oil in the species and is biosynthesized via γ-terpinene as a penultimate precursor. Different cytochrome P450 including CYP71D178 and CYP71D182 are involved in the biosynthesis of thymol and carvacrol in thyme and oregano via the γ-terpinene as an intermediate. The aim of the present work was to study the expression patterns of genes involved in thymol and carvacrol biosynthesis including DXR, γ-terpinene synthase, CYP71D178 and CYP71D180 genes in flowers and leaves of thyme. Gene expression analysis of DXR, γ-terpinene synthase, CYP71D178 and CYP71D180 genes were carried out by semi-quantitative RT-PCR technique. The relative gene expression of flowers compared with leaves for DXR, γ-terpinene synthase, CYP71D178 and CYP71D180 genes were 1.7, 2.7, 1.9 and 2 fold higher, respectively and the gene expression intensity varied for each gene. Expression of aforementioned genes in glandular trichomes were high, hence the higher gene expression in flowers might be related to higher density of glandular trichomes. Based on these results, higher content of essential oils in flowers compared to leaves is most likely further related to the higher expression of related genes in flowers rather than leaves. | ||
| کلیدواژهها [English] | ||
| DXR, Thymus vulgaris, γ-terpinene, CYP71D178, CYP71D182, Gene expression | ||
| مراجع | ||
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