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جدا سازی ژن کلیدی تری بتا هیدروکسی استروئید دهیدروژناز (Δ5-3β-hydroxysteroid dehydrogenase) در بیوسنتز کاردنولیدها و بررسی بیان آن تحت تأثیر الیسیتورهای سالیسیلیک اسید و متیل جاسمونات در گیاه دارویی گل انگشتانه ایرانی (Digitalis nervosa L) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 8، دوره 25، شماره 1 - شماره پیاپی 49، خرداد 1396، صفحه 97-110 اصل مقاله (284.34 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2017.109824 | ||
| نویسندگان | ||
| اسعد معروفی* 1؛ وحیده سلیمی2؛ محمد مجدی3 | ||
| 1استادیار، دانشکده کشاورزی، دانشگاه کردستان، سنندج | ||
| 2دانش‌آموخته کارشناسی ارشد، بیوتکنولوژی کشاورزی، دانشگاه کردستان، سنندج | ||
| 3دانشیار، دانشکده کشاورزی، دانشگاه کردستان، سنندج | ||
| چکیده | ||
| گل انگشتانه از منابع مهم ترکیبات دارویی گلیکوزیدهای قلبی مانند دیگوکسین، دیژیتوکسین و لاناتوزیدها میباشند که در نارسایهایی احتقانی قلب کاربرد درمانی دارند. جنس Digitalis دارای چندین گونه با ارزش است و تنها گونه بومی ایران گونه نروسا (Digitalis nervosa) میباشد که تعدادی گلیکوزید قلبی در آن شناسایی شده است. نظر به اهمیت مطالعه و بررسی گیاهان بومی بهمنظور استفاده در صنایع دارویی، این پژوهش بر روی گونه نروسا انجام شد. یکی از ژنهای کلیدی مهم در مسیر بیوسنتز کاردنولیدها در گیاه گل انگشتانه ژن 3β-HSDمیباشد که کدکننده آنزیم 3β-HSD است که در مراحل اولیه بیوسنتز کاردنولیدها نقش کلیدی دارد. هدف این تحقیق توالییابی، بررسیهای فیلوژنتیکی و بیان ژن 3β-HSD در بافت‌های مختلف و تحت تیمار با الیسیتورهای سالیسیلیک اسید و متیل جاسمونات بود. نتایج بهدست آمده آشکار کرد که توالی کدکننده این آنزیم با گونه Digitalis lanata قرابت بسیار نزدیکی دارد. در بررسی بیان ژن 3β-HSD در سطح رونوشت نیز مشخص شد که در بافت برگ دارای میزان بیان چشمگیری است و تحت تیمارهای متیل جاسمونات و سالیسیلیک اسید میزان بیان این ژن افزایش مییابد. نتایج حاصل از این پژوهش میتواند در راستای افزایش کاردنولیدها از طریق مهندسی متابولیک و دست‌ورزی ژنتیکی مفید واقع شود. | ||
| کلیدواژه‌ها | ||
| تری‌بتا هیدروکسی استروئید دهیدروژناز؛ گل انگشتانه؛ گلیکوزیدهای قلبی؛ سالیسیلیک اسید؛ متیل جاسمونات | ||
| عنوان مقاله [English] | ||
| Isolation of Δ5-3β-hydroxysteroid dehydrogenase involved in the biosynthetic pathway of cardenolides and its expression level under the influence of salicylic acid and methyl jasmonate elicitors in foxglove (Digitalis nervosa L) | ||
| نویسندگان [English] | ||
| A. Maroufi1؛ V. Salimi2؛ M. Majdi3 | ||
| 1Assist. Prof., Department of Agronomy and Plant Breeding, University of Kurdistan, Sanandaj, I.R. Iran | ||
| 2M.Sc., Agricultural Biotechnology, University of Kurdistan, Sanandaj, I.R.Iran | ||
| 3Assoc. Prof., Department of Agronomy and Plant Breeding, University of Kurdistan, Sanandaj, I.R.Iran | ||
| چکیده [English] | ||
| Foxglove plants are important sources of medicinal cardiac glycosides compounds such as digoxin and digitoxin, which are used for treatment of congestive heart failure and cardiac arrhythmia. Digitalis has several valuable species but the only species native to Iran is digitalis nervosa. Considering the importance of study on native plants in order to be used in pharmaceutical industry this research was carried out. One of the key genes in the biosynthesis of digitalis cardenolide in plants is 3β-HSD, which encodes the enzyme 3β-HSD with important role in early stage of the cardenolide biosynthesis pathway. Purpose of the present study was to determine nucleotide sequence of 3β-HSD, phylogenetic analyses and its expression under influence of salicylic acid and methyl jasmonate elicitors in different tissues. Results revealed that a sequence encoding the enzyme show very close affinity to Digitalis lanata. In addition expression pattern of 3β-HSD in the transcript level revealed that a significant expression level was detected in leaf tissue, and also under treatment of methyl jasmonate and salicylic acid the expression level raised. Findings of our study could be used to increase cardenolide through metabolic engineering and genetic manipulation. | ||
| کلیدواژه‌ها [English] | ||
| Cardiac glycosides, foxglove, methyl jasmonate, salicylic acid, Δ5-3β-hydroxysteroid dehydrogenase | ||
| مراجع | ||
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