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زیرگونه جدیدی از سنجد تلخ (Elaeagnus rhamnoides (L.) A.Nelson) برای ایران بر اساس دادههای مولکولی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 5، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 208-220 اصل مقاله (528.39 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109411 | ||
| نویسندگان | ||
| حمید آهنی* 1؛ جلیلوند حمید2؛ جمیل واعظی3؛ سیّد احسان ساداتی4 | ||
| 1اداره کل منابع طبیعی، استان خراسان رضوی، مشهد، | ||
| 2استاد گروه جنگلداری دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران | ||
| 3دانشیار، گروه زیست شناسی، دانشکده علوم، دانشگاه فردوسی مشهد | ||
| 4دانشیار، مرکز تحقیقات کشاورزی و منابع طبیعی مازندران، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری | ||
| چکیده | ||
| سنجد تلخ (Elaeagnus rhamnoides (L.) A.Nelson) گیاهی دارویی، مقاوم به خشکی، تثبیتکننده ازت و یک گونه پیشآهنگ جنگلی است. در این پژوهش برای اولین بار در ایران، شناسایی مولکولی سنجد تلخ در رویشگاههای مختلف کشور و مقایسه آنها با زیرگونههای شناخته شده در سایر نقاط جهان انجام شد. از پنج رویشگاه در استانهای البرز، مازندران، آذربایجانشرقی و غربی و قزوین نمونهبرداری انجام گردید. از نشانگر هستهای ITS ریبوزومی در مطالعه مولکولی استفاده شد. پس از استخراج DNA و انجام واکنش زنجیرهای پلیمراز، توالیهای بهدست آمده از این مطالعه بههمراه توالیهای بانک ژن مربوط به زیرگونههای سنجد تلخ در سایر نقاط دنیا، در یک ماتریس همردیف شده و مورد تجزیهوتحلیل فیلوژنتیکی Bayesian و شبکه TCS قرار گرفتند. نتایج حاصل از این مطالعه نشان داد که نمونههای جمعآوری شده از استانهای البرز، مازندران، آذربایجانشرقی و قزوین متعلق به زیرگونه قفقازی E. rhamnoides subsp. caucasica هستند، اما نمونه جمعآوری شده از استان آذربایجانغربی با دو تفاوت نوکلئوتیدی بهعنوان یک زیرگونه جدید و با نامE. rhamnoides subsp. azerbaijanica معرفی میشود. | ||
| کلیدواژهها | ||
| تجزیهوتحلیل فیلوژنتیکی؛ سنجد تلخ؛ شناسایی مولکولی؛ گیاه دارویی | ||
| عنوان مقاله [English] | ||
| A new subspecies of sea buckthorns (Elaeagnus rhamnoides (L.) A. Nelson) from Iran based on molecular data | ||
| نویسندگان [English] | ||
| H. Ahani1؛ H. Jalilvand2؛ J. Vaezi3؛ S.E. Sadati4 | ||
| 1Natural Resources Office of Khorasan Razavi, Mashhad, I.R. Iran | ||
| 2Professor, Department of Forestry, natural resources faculty, Sari agricultural sciences and natural resources University, Iran | ||
| 3Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, I.R. Iran | ||
| 4- Assoc. Prof., Agriculture and Natural Resources Research Center of Mazandaran, Agricultural Research, Education and Extension Organization (AREEO), Sari, I.R. Iran | ||
| چکیده [English] | ||
| Sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) is a medicinal, drought tolerant, nitrogen fixing and forest pioneer plant species. In this research, for the first time in Iran, molecular identification of the species distributed in different habitats of the country was carried out comparing to the known subspecies distributed worldwide. Specimens were collected from five habitats located in Alborz, Mazandaran, East and West Azerbaijan, and Qazvin provinces. Nuclear ribosomal ITS marker was used in the molecular study. After DNA extraction and PCR application, sequences obtained in this study were aligned with those obtained from Gene-Bank belonging to all sea buckthorn subspecies. The aligned sequences were analyzed using Bayesian and TCS phylogenetic methods. Results showed that the specimens collected from Alborz, Mazandaran, East Azerbaijan and Qazvin belong to the Caucasian subspecies, E. rhamnoides subsp. caucasica, but the specimen sampled from West Azerbaijan with two mutations is a new subspecies. Therefore, it is introduced as E. rhamnoides subsp. azerbaijanica. | ||
| کلیدواژهها [English] | ||
| Elaeagnus rhamnoides, medicinal plant, molecular identification, Phylogenetic analysis | ||
| مراجع | ||
|
-Altschul, S.F., Gish, W., Miller, E.W. and Lipman, D.J., 1990. Basic local alignment search tool. Journal of Molecular Biology, 215: 403-410. -Bestwick, C.S., Milne, L. and Duthie, S.J., 2007. Kaempferol induced inhibition of HL-60 cell growth results from a heterogeneous response, dominated by cell cycle alterations. Chemico-Biological Interactions, 170: 76-85. -Clement, M., Posada, D. and Crandall, K. 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology, 9: 1657-1660. -Dehghani, M. and Ahmadi, K., 2011. The effect of Sea Buckthorns and Wild Rue extracts on the length of nymphal and puparium of greenhouse whitefly (Trialeurodes vaporariorum). Journal of Herbal Drugs, 2(4): 239-244. -Doyle, J.J. and Doyle, J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19: 11-15. -Fareghi, SH., Farshadfar, E. and Farshadfar, M., 2007. Assessment of some perennial medics (Medicago sativa L.) based on chemical minerals and food substances and their genetic diversity investigation using SDS-PAGE marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(3): 120-196. -Geetha, S. and Asheesh, G., 2011. Review Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.). Journal of Ethnopharmacology, 138: 268–278. -Ghahreman, A., 1993. Cormophytes of Iran. Vol. 2. Markaze Nashre Daneshgahi Press, 843 pp. -Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41:95-98. -Huelsenbeck, J.P. and Ronquist, F., 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics 17: 754-755. -Javanshir, K., 1976. Atlas of Iranian wood plants. National Community of Natural Resources and Human Environment Conservation, 163 pp. -Jeppsson, N., Bartish, I.V. and Persson, H.A., 1999. DNA analysis as a Tool in Sea Buckthorn Breeding, Reprinted from. In: Perspectives on New Crops and New Uses. Janick, J., (eds), ASHS Press, Alexandria, VA, 528 pp. -Jia, D.R., Abbott, R.J., Liu, T.L., Mao, K.S., Bartish, I.V. and Liu, J.Q., 2012. Out of the Qinghai–Tibet Plateau: evidence for the origin and dispersal of Eurasian temperate plants from a phylogeographic study of Hippophae rhamnoides (Elaeagnaceae). New Phytologist, 194: 1123-1133. -Li, D.Z., Gao, L.M., Li,H.T., Wang, H., Ge, X.J., Liu, J.Q., Chen, Z.D., Zhou, S.L., Chen, S.L., Yang, J.B., Fu, C.X., Zeng, C.X., Yan, H.F., Zhu, Y.J., Sun, Y.S., Chen, S.Y., Zhao, L., Wang, K., Yang, T. and Duan, G.W., 2011. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants, Hippophae rhamnoides subsp. gyantsensis voucher JF976624. Proceedings of the National Academy of Sciencs U.S.A., 108 (49): 19641-19646. -Marvi Mohajer, M.R., 2006. Silvics and Forest Training. Tehran University Press, 387 pp. -Mashaekhi, Sh., Shiran, B., Jahanbazi Gozani, H., Hooshmand, S., Soltani, E. and Sorkheh, K., 2010. Genetic biodiversity assessment of Iranian oak in Charmahal-o-Bakhtiari province using AFLP molecular marker. Journal of Forest and Wood Product, 63(1): 77-90. -Michel, T., Destandau, E., Floch, G., Elisabeth Lucchesi, M. and Elfakir, C., 2012. Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed. Food Chemistry, 131: 754-760. -Mohammadi Faresani, T., Etemadi, N. and Seyyed Tabatabaei, BA., 2008. Genetic diversity assessment of scutch grass specimens (Cynodon dactylon) using morphological characters and ISSR molecular markers. Iranian Journal of Horticultural Science and Technology, 9(2): 83-96. -Mozaffarian, VA., 2004. Trees and Shrubs of Iran. Farhange Moa’ser Press. 1003 pp. -Mozaffarian, VA., 2012. Identification of Medicinal and Aromatic Plants of Iran. Farhange Moa’ser press. 1444 pp. -Murray, E., 1984. Eleaegnaceae. In: Rechinger, K.H., (Ed.). Flora Iranica. Vol. 55. Akademische druck- und verlagsanstalt, Graz. -Nelson, A. 1935. 'Elaeagnus rhamnoides L. (A.Nelson)'. American Journal of Botany, 22(7): 682. -Nylander, J.A.A., 2004. MrModeltest v2. Program distributed by the author, Evolutionary Biology Centre, Uppsala University. Available from http://www.abc.se/~nylander. -Page, D.M., 2001. TreeView (Win32) Version 1.6.6. Available from http://taxonomy.zoology.gla.ac.uk/rod -Rambaut, A. and Drummond, A.J., 2007. Tracer, version 1.4. Available from: http:// beast.bio.ed.ac.uk/tracer. Ruan, C., Qin, P., Zheng, J. and He, Z., 2004. Genetic relationships among some cultivars of sea buckthorn from China, Russia and Mongolia based on RAPD analysis. Scientia Horticulturae, 101: 417-426. -Sabeti, H., 1994. Forests, Trees and Shrubs of Iran. Yazd University Press, 1009 pp. -Sadeghi Pourroudsari, H.R., Haeri Rouhani, S.A., Parandin, R., Vosoughi, M., Sepehri, H., Hagi Akhoundi, A. and Khanavi, M., 2006. Study of antifertility effects of Sea Buckthorns (Melia azedarach L.) fruit oil distributed in Iran on desert male rats. Herbal Drugs, 5(18): 23-29. -Seyyed Tabatabaei, B.A., Rahim Malek, M., Talebi Bodaf, M., Yamchi, A., Etemadi, N. and Mobli, M., 2007. Genetic diversity assessment of elm populations (Ulmus spp. L.) of Isfahan using RAPD and ISSR markers. Iranian Journal of Horticultural Science and Technology, 8(4): 213-224. -Shah, A. H., Ahmad, S.D., Khaliq, I., Batool, F., Hassan, L. and Pearce, S.R., 2009. Evaluation of phylogenetic relationship among Seabuckthorn (ssp. turkestanica) wild ecotypes from Pakistan using AFLP Pakistan Journal of Botany, 41(5): 2419-2426. -Sun, K., Chen, X., Ma, R., Li, C., Wang, Q. and Ge, S., 2002. Molecular phylogenetics of Hippophae L. (Elaeagnaceae) based on the internal transcribed spacer (ITS) sequences of nrDNA. Plant Systematics and Evolution, 235: 121-134. -Swofford, D.L., 2002. PAUP*: phylogenetic analysis using parsimony (*and other methods), version 4.0b10. Sinauer Press, Sunderland, Mass. -Tang, X., 2002. Intrinsic change of physical and chemical properties of sea buckthorn (Hippophae rhamnoides L.) and implications for berry maturity and quality. Journal of Horticultural science and Technology, 77: 177-185. -Thompson, J.D., Higgins, D.G. and Gibson, T.J., 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22: 4673-4680. -White, T.J., Bruns, T., Lee, S. and Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. and White,T.J., (eds) PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 315-322. -Zhang, H.Q., Tang, M., Chen, H., Tian, Z.Q., Xue, Y.Q. and Feng, Y., 2010. Communities of arbuscular mycorrhizal fungi and bacteria in the rhizosphere of Caragana korshinkii and Hippophae rhamnoides in Zhifanggou watershed. Plant and Soil, 326: 415-424. -Altschul, S.F., Gish, W., Miller, E.W. and Lipman, D.J., 1990. Basic local alignment search tool. Journal of Molecular Biology, 215: 403-410. -Bestwick, C.S., Milne, L. and Duthie, S.J., 2007. Kaempferol induced inhibition of HL-60 cell growth results from a heterogeneous response, dominated by cell cycle alterations. Chemico-Biological Interactions, 170: 76-85. -Clement, M., Posada, D. and Crandall, K. 2000. TCS: a computer program to estimate gene genealogies. Molecular Ecology, 9: 1657-1660. -Dehghani, M. and Ahmadi, K., 2011. The effect of Sea Buckthorns and Wild Rue extracts on the length of nymphal and puparium of greenhouse whitefly (Trialeurodes vaporariorum). Journal of Herbal Drugs, 2(4): 239-244. -Doyle, J.J. and Doyle, J.L., 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry Bulletin, 19: 11-15. -Fareghi, SH., Farshadfar, E. and Farshadfar, M., 2007. Assessment of some perennial medics (Medicago sativa L.) based on chemical minerals and food substances and their genetic diversity investigation using SDS-PAGE marker. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(3): 120-196. -Geetha, S. and Asheesh, G., 2011. Review Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.). Journal of Ethnopharmacology, 138: 268–278. -Ghahreman, A., 1993. Cormophytes of Iran. Vol. 2. Markaze Nashre Daneshgahi Press, 843 pp. -Hall, T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41:95-98. -Huelsenbeck, J.P. and Ronquist, F., 2001. MrBayes: Bayesian inference of phylogeny. Bioinformatics 17: 754-755. -Javanshir, K., 1976. Atlas of Iranian wood plants. National Community of Natural Resources and Human Environment Conservation, 163 pp. -Jeppsson, N., Bartish, I.V. and Persson, H.A., 1999. DNA analysis as a Tool in Sea Buckthorn Breeding, Reprinted from. In: Perspectives on New Crops and New Uses. Janick, J., (eds), ASHS Press, Alexandria, VA, 528 pp. -Jia, D.R., Abbott, R.J., Liu, T.L., Mao, K.S., Bartish, I.V. and Liu, J.Q., 2012. Out of the Qinghai–Tibet Plateau: evidence for the origin and dispersal of Eurasian temperate plants from a phylogeographic study of Hippophae rhamnoides (Elaeagnaceae). New Phytologist, 194: 1123-1133. -Li, D.Z., Gao, L.M., Li,H.T., Wang, H., Ge, X.J., Liu, J.Q., Chen, Z.D., Zhou, S.L., Chen, S.L., Yang, J.B., Fu, C.X., Zeng, C.X., Yan, H.F., Zhu, Y.J., Sun, Y.S., Chen, S.Y., Zhao, L., Wang, K., Yang, T. and Duan, G.W., 2011. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants, Hippophae rhamnoides subsp. gyantsensis voucher JF976624. Proceedings of the National Academy of Sciencs U.S.A., 108 (49): 19641-19646. -Marvi Mohajer, M.R., 2006. Silvics and Forest Training. Tehran University Press, 387 pp. -Mashaekhi, Sh., Shiran, B., Jahanbazi Gozani, H., Hooshmand, S., Soltani, E. and Sorkheh, K., 2010. Genetic biodiversity assessment of Iranian oak in Charmahal-o-Bakhtiari province using AFLP molecular marker. Journal of Forest and Wood Product, 63(1): 77-90. -Michel, T., Destandau, E., Floch, G., Elisabeth Lucchesi, M. and Elfakir, C., 2012. Antimicrobial, antioxidant and phytochemical investigations of sea buckthorn (Hippophaë rhamnoides L.) leaf, stem, root and seed. Food Chemistry, 131: 754-760. -Mohammadi Faresani, T., Etemadi, N. and Seyyed Tabatabaei, BA., 2008. Genetic diversity assessment of scutch grass specimens (Cynodon dactylon) using morphological characters and ISSR molecular markers. Iranian Journal of Horticultural Science and Technology, 9(2): 83-96. -Mozaffarian, VA., 2004. Trees and Shrubs of Iran. Farhange Moa’ser Press. 1003 pp. -Mozaffarian, VA., 2012. Identification of Medicinal and Aromatic Plants of Iran. Farhange Moa’ser press. 1444 pp. -Murray, E., 1984. Eleaegnaceae. In: Rechinger, K.H., (Ed.). Flora Iranica. Vol. 55. Akademische druck- und verlagsanstalt, Graz. -Nelson, A. 1935. 'Elaeagnus rhamnoides L. (A.Nelson)'. American Journal of Botany, 22(7): 682. -Nylander, J.A.A., 2004. MrModeltest v2. Program distributed by the author, Evolutionary Biology Centre, Uppsala University. Available from http://www.abc.se/~nylander. -Page, D.M., 2001. TreeView (Win32) Version 1.6.6. Available from http://taxonomy.zoology.gla.ac.uk/rod -Rambaut, A. and Drummond, A.J., 2007. Tracer, version 1.4. Available from: http:// beast.bio.ed.ac.uk/tracer. | ||
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