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زیرگونه جدیدی از سنجد تلخ (Elaeagnus rhamnoides (L.) A.Nelson) برای ایران بر اساس داده‌های مولکولی | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 5، دوره 24، شماره 2 - شماره پیاپی 48، آذر 1395، صفحه 208-220 اصل مقاله (528.39 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2016.109411 | ||
| نویسندگان | ||
| حمید آهنی* 1؛ جلیلوند حمید2؛ جمیل واعظی3؛ سیّد احسان ساداتی4 | ||
| 1اداره کل منابع طبیعی، استان خراسان رضوی، مشهد، | ||
| 2استاد گروه جنگلداری دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ایران | ||
| 3دانشیار، گروه زیست شناسی، دانشکده علوم، دانشگاه فردوسی مشهد | ||
| 4دانشیار، مرکز تحقیقات کشاورزی و منابع طبیعی مازندران، سازمان تحقیقات، آموزش و ترویج کشاورزی، ساری | ||
| چکیده | ||
| سنجد تلخ (Elaeagnus rhamnoides (L.) A.Nelson) گیاهی دارویی، مقاوم به خشکی، تثبیتکننده ازت و یک گونه پیش‌آهنگ جنگلی است. در این پژوهش برای اولین بار در ایران، شناسایی مولکولی سنجد تلخ در رویشگاههای مختلف کشور و مقایسه آنها با زیرگونههای شناخته شده در سایر نقاط جهان انجام شد. از پنج رویشگاه در استانهای البرز، مازندران، آذربایجان‌شرقی و غربی و قزوین نمونهبرداری انجام گردید. از نشانگر هستهای ITS ریبوزومی در مطالعه مولکولی استفاده شد. پس از استخراج DNA و انجام واکنش زنجیرهای پلیمراز، توالیهای بهدست آمده از این مطالعه بههمراه توالیهای بانک ژن مربوط به زیرگونههای سنجد تلخ در سایر نقاط دنیا، در یک ماتریس هم‌ردیف شده و مورد تجزیه‌وتحلیل فیلوژنتیکی Bayesian و شبکه TCS قرار گرفتند. نتایج حاصل از این مطالعه نشان داد که نمونههای جمعآوری شده از استانهای البرز، مازندران، آذربایجان‌شرقی و قزوین متعلق به زیرگونه قفقازی E. rhamnoides subsp. caucasica هستند، اما نمونه جمعآوری شده از استان آذربایجان‌غربی با دو تفاوت نوکلئوتیدی بهعنوان یک زیرگونه جدید و با نامE. rhamnoides subsp. azerbaijanica معرفی میشود. | ||
| کلیدواژه‌ها | ||
| تجزیه‌وتحلیل فیلوژنتیکی؛ سنجد تلخ؛ شناسایی مولکولی؛ گیاه دارویی | ||
| عنوان مقاله [English] | ||
| A new subspecies of sea buckthorns (Elaeagnus rhamnoides (L.) A. Nelson) from Iran based on molecular data | ||
| نویسندگان [English] | ||
| H. Ahani1؛ H. Jalilvand2؛ J. Vaezi3؛ S.E. Sadati4 | ||
| 1Natural Resources Office of Khorasan Razavi, Mashhad, I.R. Iran | ||
| 2Professor, Department of Forestry, natural resources faculty, Sari agricultural sciences and natural resources University, Iran | ||
| 3Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, I.R. Iran | ||
| 4- Assoc. Prof., Agriculture and Natural Resources Research Center of Mazandaran, Agricultural Research, Education and Extension Organization (AREEO), Sari, I.R. Iran | ||
| چکیده [English] | ||
| Sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) is a medicinal, drought tolerant, nitrogen fixing and forest pioneer plant species. In this research, for the first time in Iran, molecular identification of the species distributed in different habitats of the country was carried out comparing to the known subspecies distributed worldwide. Specimens were collected from five habitats located in Alborz, Mazandaran, East and West Azerbaijan, and Qazvin provinces. Nuclear ribosomal ITS marker was used in the molecular study. After DNA extraction and PCR application, sequences obtained in this study were aligned with those obtained from Gene-Bank belonging to all sea buckthorn subspecies. The aligned sequences were analyzed using Bayesian and TCS phylogenetic methods. Results showed that the specimens collected from Alborz, Mazandaran, East Azerbaijan and Qazvin belong to the Caucasian subspecies, E. rhamnoides subsp. caucasica, but the specimen sampled from West Azerbaijan with two mutations is a new subspecies. Therefore, it is introduced as E. rhamnoides subsp. azerbaijanica. | ||
| کلیدواژه‌ها [English] | ||
| Elaeagnus rhamnoides, medicinal plant, molecular identification, Phylogenetic analysis | ||
| مراجع | ||
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Available from http://www.abc.se/~nylander. -Page, D.M., 2001. TreeView (Win32) Version 1.6.6. Available from http://taxonomy.zoology.gla.ac.uk/rod -Rambaut, A. and Drummond, A.J., 2007. Tracer, version 1.4. Available from: http:// beast.bio.ed.ac.uk/tracer. | ||
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