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Hassanloo, Thahereh, Jafarkhani Kermani, Maryam, Malmir Chegini, Mahsa, Sepehrifar, Roshanak, Mohajeri Naraghi, Sepehr, Miri, Seyyed Mehdy. (1394). Optimization of in vitro Propagation of Qare-Qat (Vaccinium arctostaphylus). سامانه مدیریت نشریات علمی, 4(2), 225-231. doi: 10.22092/jmpb.2015.108913
Thahereh Hassanloo; Maryam Jafarkhani Kermani; Mahsa Malmir Chegini; Roshanak Sepehrifar; Sepehr Mohajeri Naraghi; Seyyed Mehdy Miri. "Optimization of in vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)". سامانه مدیریت نشریات علمی, 4, 2, 1394, 225-231. doi: 10.22092/jmpb.2015.108913
Hassanloo, Thahereh, Jafarkhani Kermani, Maryam, Malmir Chegini, Mahsa, Sepehrifar, Roshanak, Mohajeri Naraghi, Sepehr, Miri, Seyyed Mehdy. (1394). 'Optimization of in vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)', سامانه مدیریت نشریات علمی, 4(2), pp. 225-231. doi: 10.22092/jmpb.2015.108913
Hassanloo, Thahereh, Jafarkhani Kermani, Maryam, Malmir Chegini, Mahsa, Sepehrifar, Roshanak, Mohajeri Naraghi, Sepehr, Miri, Seyyed Mehdy. Optimization of in vitro Propagation of Qare-Qat (Vaccinium arctostaphylus). سامانه مدیریت نشریات علمی, 1394; 4(2): 225-231. doi: 10.22092/jmpb.2015.108913

Optimization of in vitro Propagation of Qare-Qat (Vaccinium arctostaphylus)

Journal of Medicinal plants and By-product
مقاله 14، دوره 4، شماره 2، آذر 2015، صفحه 225-231    اصل مقاله (1.59 M)
شناسه دیجیتال (DOI): 10.22092/jmpb.2015.108913
نویسندگان
Thahereh Hassanloo* 1؛ Maryam Jafarkhani Kermani2؛ Mahsa Malmir Chegini3؛ Roshanak Sepehrifar1؛ Sepehr Mohajeri Naraghi1؛ Seyyed Mehdy Miri2
1Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran Karaj, Iran
2Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran Karaj, Iran
3Dpartment of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran
چکیده
In order to optimize the micropropagation of Qare-Qat (Vaccinium arctostaphylus J.J.Sm), samples were collected from two regions of Iran (Asalem and Kelardasht). Anderson (AN) media containing different concentrations of zeatin (0, 0.5, 1, 2 and 4 mg L-1) and different levels of pH (4.5, 5 and 5.5) were compared. The in vitro shoots were transferred on AN media supplemented with zeatin (1 and 2 mg L-1) alone or in combination with Indole-3-butyric acid (IBA) (0.2 and 0.4 mg L-1). To optimize the rooting stage, elongated shoots (1.5- 2cm) were cultured on half strength semi- solid media containing activated charcoal (0 and 7g L-1), or half strength liquid AN medium supplemented with different concentrations of IBA (0, 0.5, 2 and 3 mg L-1) and NAA (0 and 0.5 mg L-1). The results indicated that at the establishment stage, AN media supplemented with 4 mg L-1 zeatin (pH= 5.5) and 1 mg L-1 zeatin (pH= 5) were the best treatment for Asalem and Kelardasht explants, respectively. For the Asalem explants, the highest number of proliferated shoots (4) was observed on AN media consisting of 2 mg L-1 zeatin and 0.4 mg L-1 IBA. Whereas for the Kelardash explants  the highest number of proliferated shoots (3) was observed on AN media consisting of 2 mg L-1 zeatin and 0.2 mg L-1 IBA. The highest percent of root formation (86 and 66%) were observed in half strength AN medium containing 7 g L-1 activated charcoal and 3 mg L-1 IBA and 0.5 mg L-1 NAA in Asalem and Kelardasht explants, respectively. The plantlets were successfully transferred to soil and the survival rate was 85%.
کلیدواژه‌ها
Medicinal plants؛ Micropropagation؛ in vitro؛ growth regulators؛ Vaccinium arctostaphylus
مراجع
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