| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 2,784 |
| تعداد مقالات | 31,679 |
| تعداد مشاهده مقاله | 40,110,146 |
| تعداد دریافت فایل اصل مقاله | 17,604,366 |
Sexual Propagation of Persian Shallot (Allium hirtifolium) Through Manual Pollination and In vitro Ovary Germination | ||
| Journal of Medicinal plants and By-Products | ||
| مقاله 8، دوره 2، شماره 1، تیر 2013، صفحه 57-60 اصل مقاله (297.1 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2013.108491 | ||
| نویسندگان | ||
| Ali Jafari-Mofidabadi* 1؛ Iman Jafari2؛ Tayebe Shomali3 | ||
| 1Golestan Research Center of Agriculture and Natural Resources, P.O Box 4915677555 Gorgan, Iran | ||
| 2Seed & Plant Certification and Registration Institute, Karaj, Iran | ||
| 3Mazandran University of Medical Sciences, Faculty of Pharmacy, Sari, Iran | ||
| چکیده | ||
| For sexual reproduction of Allium hirtifolium, collected bulbs were planted in the field. In order to develop ovary embryo and seed setting, control self-pollination and natural open pollination took placed using honey bees under cages. Ovary development and seed setting of A. hirtifolium extraordinary were affected by pollination and there was a significant difference between mean effect of self-pollination and natural open pollination using chi-Square test at α=0.05%. Open pollination using honey bees with average 23 numbers of seeds per flower stalk showed higher seed formation than control self- pollination. Out of 240 mature ovaries (25 and 35 day-old ovary), which have been isolated, 194 ovary were germinated (80.8%) from 25 and 35 days after pollination (DAP). Due to long embryo development in this species, all isolated embryo (less than 25 day-old) failed to develop direct plantlets on either MS hormone free or half-MS medium, for in vitro germination. Analysis of data originated from affect of culture media on embryo germination, indicated that there is no significant differences between culture media on embryo germination using Chi-square test at α=0.05 level. In spite of no differences, MS media showed higher ovary germination (average 54.33%) than half-MS medium (average 41.50%). Successful acclimatized plantlets transferred to green-house and then to the field. | ||
| کلیدواژه‌ها | ||
| Allium hirtifolium؛ Mooseer؛ OVARY؛ Embryo؛ Sexual reproduction؛ Pollination | ||
| مراجع | ||
|
1. Moradi AR. Hybridization of diploid and tetraploid cottons through in vitro embryo culture. M.Sc Thesis. Tarbiat Modares University, 1997.
2. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant.1962;15:473–497.
3. Jafari MA, Modir-Rahmati A. Production of Populus euphratica Oliv. × P. alba L. hybrid poplars through ovary and ovule cultures. Plant Genet Newslett. 2000;122:13-15.
4. Ranga TS. Culture of ovules In: Cell Culture and Somatic Cell Genetics of Plants, Vol. 1: Laboratory Procedures and Their Applications. Ed. I.K. Vasil. Academic Press, New York. 1984, pp. 227–231.
5. Ramming D.W. The use of embryo culture in fruit breeding.HortScience 1990;25:393–398.
6. Pinto ACQ, Rogers SMD, Byrne DH. Growth of immature peach embryos in response to media, ovule support method, and ovule perforation.HortScience 1994;29:1081–1083.
7. Gill MS, Bajaj YPS. Hybridization between diploid (Gossypium arboreum) and tetraploid (Gossypium hirsutum) cotton through ovule culture. Euphytica 1987;36:625–630.
8. Hu C, Wang P. Embryo culture: Technique and application. In: Handbook of Plant Cell Culture Vol. 4. Ed. D.A. Evans, W.R. Sharp, and P.V. Ammirato. Macmillan, New York. 1986, pp. 43–96.
9. Pinto ACQ, Rogers SMD, Byrne DH. Growth of immature peach embryos in response to media, ovule support method, and ovule perforation.HortScience 1994;29:1081–1083.
10. Stewart JM, CL Hsu. Hybridization of diploid and tetraploid cottons through in-ovulo embryo culture. J Heredit. 1978;69:404-408.
11. Stewart JM C.L.Hsu. In-ovulo embryo culture and seeling development of cotton (Gossypium hirsutum L.). J. Planta 1977;2:113-115.
12. Raghavan V. Applied aspects of embryo culture, p. 375–397. In: J. Reinert and Y.P.S. Bajaj (eds.). Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer-Verlag, Berlin 1997.
13. Collins GB, Grosser JW. Culture of embryos. In:Cell Culture and Somatic Cell Genetics of PlantsVol. 1: Laboratory Procedures and Their Applications . Ed. I.K. Vasil. Academic Press, New York. 1984, pp. 241–257.
14. Hu C, Wang P. Embryo culture: Technique and application. In:Handbook of Plant Cell CultureVol. 4. Ed. D.A. Evans, W.R. Sharp, and P.V. Ammirato. Macmillan, New York. 1986, pp. 43–96.
15. Kalagari M, Jafari MA. Tabari M, Hosseini SM.: Intraspecific hybridization of Populus euphratica Oliv. using in vitro technique. J Sci. 2004;15:109-122.
16. Bridgen MP. A review of plant embryo culture .HortScience 1994;29:1243–1246.
17. Yeung EC, Thorpe TA, Jensen CJ. In vitro fertilization and embryo culture, p. 253–271. In: T.A. Thorpe (ed.). Plant tissue culture: Methods and applications in agriculture. Academic, New York, 1981.
18. Jafari MA, Modir-Rahmati A, Tavesoli A. Application of ovary and ovule culture in P. alba L x Populus euphratica OLIV hybridization. Silvae Genet. 1998;47:5-6.
19. Raghavan V. Embryo culture, p. 209–240. In: I.K. Vasil (ed.). Perspectives in plant cell and tissue culture. Intl. Rev. Cytol., Suppl. 11B. Academic, New York .1980.
20. Gamborg OL, Miller RA, Ojima K. Nutrient requirements of suspension cultures of soybean root cells.Exp Cell Res. 1968;50:151–158. | ||
|
آمار تعداد مشاهده مقاله: 464 تعداد دریافت فایل اصل مقاله: 470 |
||