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Enhancement of Tropane Alkaloid Production among Several Clones and Explants Types of Hairy Root of Atropa belladonna L. | ||
| Journal of Medicinal plants and By-Products | ||
| مقاله 6، دوره 1، شماره 1، تیر 2012، صفحه 35-42 اصل مقاله (382.23 K) | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2012.108444 | ||
| نویسندگان | ||
| Ali Eskandari-Samet1؛ Khosro Piri* 1؛ Mehrnaz Kayhanfar1؛ Tahereh Hasanloo2 | ||
| 1Department of Biotechnology - Faculty of Agriculture - Bu-Ali Sina University, Hamadan, Iran | ||
| 2Agricultural BiotechnologyResearch Institute, Karaj, Iran | ||
| چکیده | ||
| Agrobacterium rhizogenes (pRi), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species. In our study four bacterial strains AR15834, A4, 9435 and C318 and three explants types leaf, stems and roots, were examined. Hairy roots were induced from roots, stems and leaf explants. The highest transformation efficiency of 77% was achieved by using strain AR15834. The transgenic status of hairy roots was confirmed by polymerase chain reaction using rolB specific primers for the presence of rolB gene in the genomes of transformed roots. Six clones of hairy roots were established that differed in their morphology. The results indicated that 4–11 fold increasing in root biomass after 28 days compared with non-transformed seedling roots. The concentration of tropane alkaloids hyoscyamine and scopolamine obtained in transformed roots (4 mg/g dry weight) was 3-11 folds more than in non-transformed cultured roots (0.36 mg/g dry weight). | ||
| کلیدواژه‌ها | ||
| hairy root؛ Bacterial strain؛ Explants type؛ Tropane alkaloid | ||
| مراجع | ||
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1. Aoki T, Matsumoto H, Asako Y, Matsunaga Y, Shimomura K. Variation of alkaloid productivity among several clones of hairy root and regenerated plants of Atropa belladonna transformed with Agrobacterium rhizogenes 15834. Plant Cell Rep. 1997;16:282–286.
2. Ayala-Silva T, Bey CA, Dortch G. Agrobacterium rhizogenes mediated transformation of Asimina triloba L. cuttings. Pakistan J Bio Sci. 2007; 10: 132-136.
3. Babaoglu M, Davey MR, Power JB, Sporer F, Wink M. Transformed roots of Lupinus mutabilis: induction, culture and isoflavone biosynthesis. Plant Cell Tiss. Org. Cult. 2004;78:29–36.
4. Banerjee S, Zehra M, Kukreja AK, Kumar S. Hairy roots in medicinal plants. Curr. Res. Med. Arom. Plants 1995;17:348–378.
5. Baumann K, De Paolis A, Costantino P, Gualberti G. The DNA binding site of the Dof protein NtBBF1 is essential for tissue-specific and auxin-regulated expression of the rolB oncogene in plants. Plant Cell 1999;11:323–333.
6. Bonhomme V, Laurain Mattar D, Fliniaux MA. Effects of the rolC gene on hairy root: Induction development and tropane alkaloid production by Atropa belladonna. J. Nat. Prod. 2000;63:1249–1252.
7. Bourgaud F, Bouque V, Guckert A. Production of flavonoids by Psoralea hairy root cultures. Plant Cell Tiss. Org. 1999;56:97-104.
8. Bulgakov VP, Khodakovskaya MV, Labetskaya NV, Tchernoded GK, Zhuravlev YN. The impact of plant rolC oncogene on ginsenoside production by ginseng hairy root cultures. Phytochemistry 1998; 49: 1929–1934.
9. Cai D, Kleine M, Kifle S, Horloff H J, Sandal N N, Marcker K A, Lankhorst R M K , Salentijn E M J, Lange W, Stiekema W J, Wyss V, Grundler FMW, Jung C. Positional cloning of a gene for nematode resistance in sugarbeet. Science. 1997;275: 832-834.
10. Choi HK, Choi YH, Verberne M, Lefeber AWM, Erkelens C, Verpoorte R. Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique. Phytochemistry 2004; 65:857–864.
11. Christey MC, Braun RH. Production of hairy root cultures and transgenic plants by Agrobacterium rhizogenes-mediated transformation. Methods Mol. Biol. 2005;286:47–60.
12. Dhakulkar S, Bhargava S, Ganapathi TR, BapatVA. Induction of hairy roots in Gmelina arborea Roxb. using Agrobacterium rhizogenes. Founder’s Day Special Issue. 2005; pp. 100-106.
13. Doran PM. Properties and applications of hairy-root cultures. In: Okasman-Caldenty, K.-M. and BARZ, W.H., eds. Plant biotechnology and transgenic plants. New York, Mercel Dekker Inc.p. 2002;143-162.
14. Drewes FE, Staden J, van. Initiation of and solasodine production in hairy root cultures of Solanum mauritianum Scop. Plant Growth Reg. 1995;17:27-31.
15. Faiss M, Strnad M, Redig P, Dolezˇal K, Hanusˇ J, Van Onckelen H, Schmu¨ lling T. Chemically induced expression of the rolC-encoded-glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosides in planta. Plant J. 1996;10:33–46.
16. Flocco C, AlvarezL MA, Guilieti A M. Peroxidase production in vitro by Armoracia lapathifolia (horseradish)-transformed root cultures: Effect of elicitation on level and profile of isoenzymes. Biotechnol. Appl. Biochem. 1998;28:33-38.
17. Fu CX, De-Xiu Zhao DX, Xue XF, Jin ZP, Ma FS. Transformation of Saussurea involucrata by Agrobacterium rhizogenes: Hairy root induction and syringin production. Process Biochem. 2005; 40:3789–3794.
18. Giri A, Narasu ML. Transgenic hairy roots: recent trends and applications. Biotechnol. Adv. 2000;18:1-22.
19. Giri A, Ravindra ST, Dhingra V, Narasu ML. Influence of different strains of Agrobacterium rhizogenes on induction of hairy roots and artemisinin production in Artemisia annua. Curr. Sci. 2001;81:378–382.
20. Grant JE, Dommisse EM, Christey MC, Conner AJ. Gene transfer to plants using Agrobacterium, in: D.R. Murray (eds.), Advanced Methods in Plant Breeding and Biotechnology. CAB International, Wallingford. 1991; pp. 50–73.
21. Hartmann T, Witte L, Oprach F, Toppel G. Reinvestigation of the alkaloid composition of Atropa belladonna plants, root cultures and cell suspension cultures. Planta Med. 1986;52:390-395.
22. Hashimoto T, Yamada Y. Hyoscyamine 6â-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures. Plant Physiol. 1986;81: 619-625.
23. Hu ZB, Du M. Hairy root and its application in plant genetic engineering. J. Int. Plant Biol. 2006;48:121-127.
24. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey KM. Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta. 1999;208:545–551.
25. Kagei K, Hemmi S, Shirai H, Hasegawa S, Toyoshima S. Shoyakugaku Zasshi. 1978;32:222-227.
26. Kamada H, Okamura N, Satake M, Harada H, Shimomura K. Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Reports. 1986;5:239-242.
27. Kim Y, Wyslouzil B, Weathers PJ. Secondary methabolism of hairy root cultures in bioreactors. In vitro Dev. Biol. Plant. 2002;38: 1-10.
28. Kursinszki L, Hank H, László I, Szõke É. Simultaneous analysis of hyoscyamine, scopolamine, 6â-hydroxyhyoscyamine and apoatropine in Solanaceous hairy roots by reversed-phase high- performance liquid chromatography. J. Chromatogr. A. 2005;1091:32-39.
29. Lodhi AH, Charlwood BV. Agrobacterium rhizogenesmediated transformation of Rubia peregrina L.: in vitro accumulation of anthraquinones. Plant Cell Tiss. Org. Cult. 1996;46:103–108.
30. Luo C, Peng Z, Pu L. Transformation of medicinal plants mediated by Agrobacterium rhizogenes. Biotechnology. 2004;14:58-61.
31. Maldonado-Mendoza IE, Ayora-Talavera T, Loyola-Vargas VM. Tropane alkaloid production in root cultures of Datura stramonium. In vitroCell Dev. Biol. Plant. 1992;28:67-72.
32. Mano Y, Nabeshima S, Matsui C, Ohkawa H. Production of tropane alkaloids by hairy root cultures of Scopolia japonica. Agric. Biol. Chem. 1986;50:2715-2722.
33. Moyano E, Jouhikainen K, Tammela P, Palazo´ n J, Cusido´ RM, Pinˇ ol MT, Teeri TH, Oksman-Caldentey KM. Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. J. Exp. Bot. 2003;54:203–211.
34. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962;15:473–97.
35. Nilsson O, Olsson O. Getting to the root: the role of the Agrobacterium rhizogenes rol genes in the formation of hairy roots. Physiol Plant. 1997;100:463–473.
36. Nussbaumer P, Kapetanidis I, Christen P. Hairy roots of Datura candida × D. aurea: effect of culture medium composition on growth and alkaloid biosynthesis. Plant Cell Rep. 1998;17;405–409.
37. Oksman-Caldentey KM, Hiltunen R. Transgenic crops for improved pharmaceutical products. Field Crops Res. 1996;45:57-69.
38. Oksman-Caldentey K M, Arroo R. Regulation of tropane alkaloid metabolism in plants and plant cell culture. In: Metabolic engineering of plant secondary metabolism, (eds.), Verpoorte, R. and A.W. Alfermann. Kluwer Academic Publishers, Dordrecht, Netherlands. 2000; p. 253.
39. Palazo´n J, Moyano E, Cusido´ RM, Bonfill M, Oksman-Caldentey K-M, Pin˜ ol MT. Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Sci. 2003;165:1289–1295.
40. Roessner U, Luedemann A, Brust D, Fiehn O, Linke T, Willmitzer L, Fernie A R. Metabolic profiling allows comprehensive phenotyping of genetically or environmentally modified plant systems. Plant Cell. 2001;13:11-29.
41. Saito K, Yamazaki M, Anzai H, Yoneyama K, Murakoshi I. Transgenic herbicide-resistant Atropa belladonna using an Ri binary vector and inheritance oftransgenic trait. Plant Cell Rep. 1992;11:219–224.
42. Sambrook J, Fritrsch EF, ManiatisT. Molecular Cloning: A Laboratory Manual. Cold Spring Laboratory Press, Cold Spring, Harbor, NY. 1989.
43. Sevón N, Oksman-Caldentey KM. Agrobacterium rhizogenes-mediated transformation: root cultures as a source of alkaloids. Planta Med. 2002;68: 859-868.
44. Srivastava S, Sivastava AK. Hairy root culture for mass-production of high-value secondary metabolites. Crit RevBiotechnol. 2007;27: 29-43.
45. Trease GE, Evans WC. Pharmcognosy 12th edition. Eastbourne, UK: Bailliere TindaU (1983).
46. Verma PC, Laiq ur Rahman L, Negi AS, Jain DJ, Khanuja S P S, Banerjee S. Agrobacterium rhizogenes-mediated transformation of Picrorhiza kurroa Royle ex Benth.: establishmen and selection of superior hairy root clone. Plant Biotechnol. Rep. 2007;1:169–174.
47. Zhang L, Ding RX, ChaiYR, Bonfill M, Moyano E, Oksman- Caldentey K-M, Xu TF, Pi Y, Wang ZN, Zhang HM, Kai GY, Liao ZH, Sun XF, Tang KX. Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc. Natl. Acad. Sci. USA. 2004;101:6786–6791.
48. Yamada Y, Hashimoto T, Endo T. Biochemistry of alkaloid production in vitro. In Secondary products from plant tissue culture, Eds., Charlwood, B.V. and M.J.C. Rhodes. Clarendon, Oxford.1990; pp. 201-225. | ||
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