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فعالیت ضد آمیلوئیدی عصاره برگ گیاه مرزه (Satureia hortensis L.) روی فیبری شدن لیزوزیم و تأثیر احتمالی آن در درمان بیماریهای وابسته به آمیلوئید | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 32، شماره 5 - شماره پیاپی 79، دی 1395، صفحه 844-856 اصل مقاله (435.61 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.108082 | ||
| نویسندگان | ||
| حسن رامشینی* 1؛ نیره کوثری2؛ اعظم السادات مقدسی3 | ||
| 1استادیار بیوشیمی، گروه علمی زیست‌شناسی، دانشگاه پیام نور، تهران، ایران | ||
| 2کارشناسی ارشد، دانشگاه علوم پزشکی سبزوار، بیمارستان مبینی، سبزوار، ایران | ||
| 3کارشناسی ارشد، گروه علمی زیست‌شناسی، دانشگاه آزاد اسلامی، واحد دامغان، ایران | ||
| چکیده | ||
| تجمعات آمیلوئیدی از ویژگی‌های تعداد زیادی از بیماریهای تحلیل برنده سیستم عصبی ازجمله آلزایمر است. بنابراین مهار این تجمعات یک راهکار جذاب برای درمان این بیماری است. به‌طوری که با وجود تحقیقات گسترده‌ای که انجام شده است، یک روش درمانی مؤثر که به‌طور مستقیم باعث مهار این تجمعات گردد تاکنون کشف نشده است. یکی از روش‌های درمانی مهم برای جلوگیری از بروز این بیماری‌ها استفاده از گیاهان دارویی (غنی از ترکیب‌های آروماتیک) می‌باشد. در این مطالعه فعالیت مهاری عصاره برگ گیاه مرزه (Satureia hortensis L.) روی روند فیبریلاسیون لیزوزیم سفیده تخم‌مرغ مورد مطالعه قرار گرفته است. لیزوزیم با غلظت 2 میلی‌گرم بر میلی‌لیتر در بافر گلایسین 50 میلی‌مولار با 5/2pH= تهیه و در دمای 57 درجه به‌طوری که مدام هم زده می‌شود تبدیل به فیبر شد. برای اثبات تشکیل فیبرها از تکنیک‌های مختلفی ازجمله سنجش تیوفلاوین T، فلورسانس خارجی و عکس میکروسکوپ نیروی اتمی استفاده شد. تحلیل داده‌ها با استفاده از آزمون آماری تی مستقل و با نرم‌افزار SPSS.16 انجام گردید.در غیاب عصاره مرزهبعد از 48 ساعت انکوباسیون فیبرهای بالغ آمیلوئیدی ظاهر شد. در حضور عصاره در دامنه غلظت 1-25/0 میلی‌گرم بر میلی‌لیتر تشکیل فیبرهای آمیلوئیدی مهار شد. اثر مهاری وابسته به غلظت بود و بهترین غلظت مؤثر 1 میلی‌گرم بر میلی‌لیتر بدست آمد. عصاره روی تمام مراحل تشکیل فیبر شامل فازهای تأخیر، رشد و تعادل تأثیر می‌گذارد. مطالعه سمّیت سلولی به روش MTT در حضور عصاره در مقایسه با عدم حضور آن نشان داد که سمّیت آنها به‌طور معنی‌داری (05/0>P) کاهش می‌یابد. با توجه به نقش مهاری عصاره روی سمّیت سلولی و اثر آن روی تمامی مراحل مختلف تشکیل فیبر می‌توان از آن در آینده به‌عنوان یک مهارکننده احتمالی برای درمان این نوع از بیماری‌ها استفاده کرد. | ||
| کلیدواژه‌ها | ||
| تجمعات آمیلوئیدی؛ عصاره مرزه (Satureia hortensis L.)؛ لیزوزیم سفیده تخم‌مرغ؛ سمّیت سلولی | ||
| عنوان مقاله [English] | ||
| Anti-amyloidogenic activity of Satureja hortensis L. extract on lysozyme fibrillogenesis and its possible influence on amyloidal diseases treatment | ||
| نویسندگان [English] | ||
| H. Ramshini1؛ N. Kovsari2؛ A. Moghaddasi3 | ||
| 1Department of Biology, Payam Noor University, Tehran, Iran | ||
| 2Mobini Hospital, Sabzevar University of Medical Sciences, Sabzevar, Iran | ||
| 3Department of Biology, Azad University, Branch of Damghan, Damghan, Iran | ||
| چکیده [English] | ||
| Amyloid aggregate plaques are the hallmarks of many neurodegenerative disorders including Alzheimer’s disease. Thus, reducing amyloid deposits is an attractive approach to improve the therapeutic arsenal for the diseases. Despite of intensive research carried out, there is no therapeutic agent directed towards inhibition of aggregation formation. One important approach in the development of therapeutics is the use of herbal extracts, which are rich in aromatic small molecules. This study aimed to explore the inhibitory activity of Satureia hortensis extract against the fibril formation of hen egg-white lysozyme. In this experimental study, amyloid fibrillation was formed in hen egg-white lysozyme under acidic conditions at high temperature. Lysozyme (2 mg/mL) was dissolved in glycine buffer 50 mM, pH=2.5 and incubated at 57°C for the specified durations, while being gently stirred. Various techniques including thioflavin T, ANS fluorescence assay, and atomic force microscopy were used to confirm fibrillation. Data were analyzed through SPSS 16 using descriptive statistics as well as independent t-test.In the absence of Satureia hortensis, mature amyloid aggregates became evident after 48 hours of incubation but upon incubation with various extract concentrations (ranging from 0.25 to 1 mg/ml), formation of amyloid fibrils and oligomers was inhibited. The inhibition was dose-dependent and the best concentration was 1 mg/ml. Our results showed that, the extract was effective in all of amyloid formation processes including lag, polymerization, and equilibrium phases. Also, cytotoxicity of oligomers in the presence of the extract significantly (P<0.05) decreased, as indicated by the MTT assay. Based on inhibitory role of the extract on cytotoxicity of amyloid fibers and its inhibitory effect on all processes of amyloid aggregation, it could be used as a novel inhibitor for Alzheimer's disease. | ||
| کلیدواژه‌ها [English] | ||
| Amyloid aggregation, Satureia hortensis extract, hen egg-white lysozyme, Cytotoxicity | ||
| مراجع | ||
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