| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 2,909 |
| تعداد مقالات | 32,812 |
| تعداد مشاهده مقاله | 43,140,872 |
| تعداد دریافت فایل اصل مقاله | 19,712,854 |
Effects of experimental Mesobuthus eupeus scorpion envenomation on chicken | ||
| Archives of Razi Institute | ||
| مقاله 4، دوره 72، شماره 1، خرداد 2017، صفحه 23-31 اصل مقاله (2.61 M) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.107493 | ||
| نویسندگان | ||
| Mohammad Khosravi* 1؛ Mansour Mayahi2؛ Seyedeh Missagh Jalali2؛ Anahita Rezaie1؛ Ahmad Taghavi Moghadam3؛ Zohreh Hosseini4؛ Seyedeh Kolsum Barzegar4؛ Somayeh Azadmanesh4 | ||
| 1Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Khuzestan, Iran | ||
| 2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Khuzestan, Iran | ||
| 3Razi Reference Laboratory of Scorpion Research, Razi Vaccine and Serum Research Institute, Ahvaz, Iran | ||
| 4Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran. | ||
| چکیده | ||
| This study aimed to evaluate the clinical, histopathological and hematological effects of Mesobuthus eupeus venom on chicken organs. Adult chickens were subcutaneously injected with five doses of M. eupeus venom (0.5, 2, 5, 10 and 20 mg/kg; four chickens per each dose). Symptoms were recorded during the experiment and blood samples were collected for hematological analysis. Moreover, a complete necropsy was performed. After macroscopic examination, tissue samples were obtained from the liver, kidneys, heart, lungs, intestines and brain of the chickens three days after venom administration. In intravenous injection, lethal dose of the venom was determined at 15 mg/kg. The first clinical, pathological and hematological symptoms in envenomated chickens were observed at M. eupeus doses of 2, 5 and 0.5 mg/kg, respectively. Hematological examination revealed a reduction in lymphocyte count following experimental envenomation, which returned to the pre-experiment level in almost all the cases. On the other hand, heterophil count was found to increase during the experimental period. In addition, erythrocyte count and hematocrit level were stable at all the intervals. Pathological examination was indicative of severe pulmonary hemorrhage, pulmonary and cerebral edema, tubular necrosis of the kidneys, hemorrhage in kidneys and heart, hyaline thrombus and congestion of the liver. According to the results of this study, poultry are resistant to the toxic effects of M. eupeus venom. | ||
| کلیدواژه‌ها | ||
| Mesobuthus eupeus؛ Venom؛ Chicken؛ Pathology؛ Hematology | ||
| عنوان مقاله [English] | ||
| اثر گزش تجربی عقرب مزوبتوس اپئوس در ماکیان | ||
| نویسندگان [English] | ||
| محمد خسروی1؛ منصور میاحی2؛ سیده میثاق جلالی2؛ آناهیتا رضایی1؛ احمد تقوی مقدم3؛ زهره حسینی4؛ سمیه آزادمنش4؛ | ||
| 1گروه پاتوبیولوژی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 2گروه علوم بالینی، دانشکده دامپزشکی، دانشگاه شهید چمران اهواز، اهواز، ایران | ||
| 3شعبه جنوب غرب موسسه تحقیقات واکسن و سرم سازی رازی، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 4دانشجو، دانشکده دامپزشکی، دانشگاه شهید چمران، اهواز، ایران | ||
| چکیده [English] | ||
| این مطالعه اثرات بالینی، هماتولوژی و پاتولوژی زهر عقرب مزوبتوس اپئوس در ماکیان را بررسی نموده است. پنج دوز از زهر مزوبتوس اپئوس (5/0، 2، 5، 10 و 20 mg/kg) به صورت زیر پوستی به ماکیان بالغ، شامل چهار قطعه در هر گروه تزریق شد. در زمان مطالعه علائم بالینی ثبت شد: نمونه خون جهت بررسی هماتولوژی اخذ شد و کالبد گشایی کامل انجام شد. سه روز پس تزریق سم، ارزیابی ظاهری انجام شد و نمونه های بافتی از کبد، کلیه، قلب، ریه، روده و مغز اخذ شد. میزان کشندگی در تجویز داخل وریدی 15 mg/kg بود. نخستین علائم بالینی، پاتولوژی و هماتولوژی به ترتیب در مقادیر 2، 5 و 5/0 mg/kg مشاهده شد. ارزیابی هماتولوژی پس از گزش تجربی بیانگر کاهش میزان لنفوسیتها پس از گزش تجربی و تقریبا بازگشت به میزان طبیعی در همه گروهها بود، درحالیکه میزان هتروفیلها طی زمان آزمایش افزایش یافته بود. علاوه بر این تعداد گلبولهای سفید و هماتوکریت در همه فواصل نمونه گیری ثابت بود. بررسی پاتولوژیک نمایانگر خونریزی شدید ریوی، ادم ریوی و مغزی، نکروز لوله های کلیوی، خونریزی از کلیه و قلب، ترومبوز هیالینی و احتقان کبد بود. با توجه نتایج ماکیان تقریبا نسبت به اثرات سمی زهر مقاوم می باشند. | ||
| کلیدواژه‌ها [English] | ||
| مزوبتوس اپئوس, زهر, ماکیان, هماتولوژی, پاتولوژی | ||
| مراجع | ||
|
Adiguzel, S., 2010. In vivo and in vitro effects of scorpion venoms in Turkey: a mini-review. Journal of Venomous Animals and Toxins including Tropical Diseases 16, 198-211. Bawaskar, H.S., Bawaskar, P.H., 2012. Scorpion sting: update. J Assoc Physicians India 60, 46-55. Boyer, L.V., Theodorou, A.A., Berg, R.A., Mallie, J., Arizona Envenomation, I., Chavez-Mendez, A., et al., 2009. Antivenom for critically ill children with neurotoxicity from scorpion stings. N Engl J Med 360, 2090-2098. Bucaretchi, F., Baracat, E.C., Nogueira, R.J., Chaves, A., Zambrone, F.A., Fonseca, M.R., et al., 1995. A comparative study of severe scorpion envenomation in children caused by Tityus bahiensis and Tityus serrulatus. Rev Inst Med Trop Sao Paulo 37, 331-336. Chippaux, J.P., 2012. Emerging options for the management of scorpion stings. Drug Des Devel Ther 6, 165-173. Cordeiro, F.F., Sakate, M., Fernandes, V., Cuyumjian, P.R., 2006. Clinical and cardiovascular alterations produced by scorpion envenomation in dogs. Journal of Venomous Animals and Toxins including Tropical Diseases 12, 19-43. Cupo, P., Hering, S.E., 2002. Cardiac troponin I release after severe scorpion envenoming by Tityus serrulatus. Toxicon 40, 823-830. Das, S., Badhe, B., Kusa Kumar, S.K., Manickam, N., Manigandan, G., 2013. Fatal scorpion envenomation: report of two cases. J Indian Acad Forensic Med 35, 404-407. Das, S., Nalini, P., Ananthakrishnan, S., Ananthanarayanan, P.H., Balachander, J., Sethuraman, K.R., et al., 1995. Scorpion envenomation in children in southern India. J Trop Med Hyg 98, 306-308. Dehesa-Dávila, M., Possani, L.D., 1994. Scorpionism and serotherapy in Mexico. Toxicon 32, 1015-1018. Dehghani, R., Khamehchian, T., 2008. Scrotum Injury by Scorpion Sting. J Arthropod-Borne Dis 2, 49–52. Emam, S.J., Khosravi, A.D., Alemohammad, A., 2008. Evalution of Hematological and urine parameters in Hemiscorpius lepturus victims referred to Razi hospital. J Med Sci 8, 306-309. Gueron, M., Margulis, G., Ilia, R., Sofer, S., 1993. The management of scorpion envenomation 1993. Toxicon 31, 1071-1076. Gueron, M., Ovsyshcher, I., 1987. What is the treatment for the cardiovascular manifestations of scorpion envenomation? Toxicon 25, 121-124. Karatas, A., 2003. Mesobuthus eupeus (Koch,1839) (Scorpiones: Buthidae) in Anatolia. Euscorpius 7, 1–7. Karnad, D.R., 1998. Haemodynamic patterns in patients with scorpion envenomation. Heart 79, 485-489. Khamechian, T., Dehghani, R., Vazirianzadeh, B., 2009. Histopathological changes induced in rat organs by the venom of Hemiscorpius lepturus (Scorpionida: Hemiscorpiidae). Bioch Cell Arch 9, 289-296. Khoobdel, M., Zahraei-Salehi, T., Nayeri-Fasaei, B., Khosravi, M., Omidian, Z., Motedayen, M.H., et al., 2013. Purification of the Immunogenic Fractions and Determination of Toxicity in Mesobuthus eupeus (Scorpionida: Buthidae) Venom. J Arthropod Borne Dis 7, 139-146. Kumar, L., Naik, S.K., Agarwal, S.S., Bastia, B.K., 2012. Autopsy diagnosis of a death due to scorpion stinging--a case report. J Forensic Leg Med 19, 494-496. Lowe, G., 2010. Two new Hemiscorpius Peters, 1861 (Scorpiones: Hemiscorpiidae) from Northern Oman. Euscorpius 91, 1-24. Mahadevan, S., 2000. Scorpion sting. Indian Pediatr 37, 504-514. Maheshwari, M., Tanwar, C.P., 2012. Scorpion bite induced myocardial damage and pulmonary edema. Heart Views 13, 16-18. Natu, V.S., Murthy, R.K., Deodhar, K.P., 2006. Efficacy of species specific anti-scorpion venom serum (AScVS) against severe, serious scorpion stings (Mesobuthus tamulus concanesis Pocock)--an experience from rural hospital in western Maharashtra. J Assoc Physicians India 54, 283-287. Nogueira, R.M.B., Sakate, M., Sangiorgio, F., Laposy, C.B., Tostes, R.A., 2007. Experimental envenomation with Crotalus durissus terrificus venom in dogs treated with antiophidic serum - part II: laboratory aspects, electrocardiogram and histopathology. Journal of Venomous Animals and Toxins including Tropical Diseases 13, 811-820. Padilla, A., Govezensky, T., Possani, L.D., Larralde, C., 2003. Experimental envenoming of mice with venom from the scorpion Centruroides limpidus limpidus: differences in mortality and symptoms with and without antibody therapy relating to differences in age, sex and strain of mouse. Toxicon 41, 959-965. Petricevich, V.L., 2010. Scorpion Venom and the Inflammatory Response. Mediators of Inflammation 2010, 16. Pinto, M.C.L., Melo, M.M., Costa, M.E.R., Labarrere, C.R., 2010. Hematological and biochemical profiles of rats submitted to experimental poisoning with Tityus serrulatus venom. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 62, 350-356. Radha, K.M., Zolfagharian, M., Kudalkar, J.D., 1998. Disseminated intravascular Coagulation and disturbances in carbohydrate andfat metabolism in acute myocardities by scorpion (Buthustamulus) venom. Indian J Med Res 87, 318- 325. Radmanesh, M., 1990. Clinical study of Hemiscorpion lepturus in Iran. J Trop Med Hyg 93, 327-332. Ribeiro, E.L., Melo, M.M., Pinto, M.C.L., Labarrère, C.R., Guimarães, P.T.C., Paes, P.R.O., et al., 2009. Hemograma de cães submetidos ao envenenamento experimental por Tityus serrulatus. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 61, 135-143. Sadeghian, H., 2003. Transient ophthalmoplegia following envenomation by the scorpion Mesobuthus eupeus. Neurology 60, 346-347. Sagheb, M.M., Sharifian, M., Moini, M., Sharifian, A.H., 2012. Scorpion bite prevalence and complications: report from a referral centre in southern Iran. Trop Doct 42, 90-91. Taghavi Moghdam, A., Masihipour, B., Bakshandeh, N., Navidpour, S., 2009. Biochemical manifestation of Mesobuthus eupeus envenomation in human. Biochem Cell Arch 9, 83-88. Tuuri, R.E., Reynolds, S., 2011. Scorpion envenomation and antivenom therapy. Pediatr Emerg Care 27, 667-672; quiz 673-665. Upadhyay, R.K., Ahmad, S., 2008. Isolation, purification and characterization of venom toxins from Indian Red Scorpion, Mesobuthus Tamulus. J Cell Tissue Res 8, 1297–1302. Wakenell, P.S., 2010. Hematology of chickens and turkeys. , Wiley-Blackwell, USA. Warrell, D.A., 2012. Venomous animals. Medicine 40, 159-163. Zayerzadeh, E., Koohi, M.K., Mirakabadi, A.Z., Fardipoor, A., Kassaian, S.E., Rabbani, S., et al., 2012. Amelioration of cardio-respiratory perturbations following Mesobuthus eupeus envenomation in anesthetized rabbits with commercial polyvalent F(ab′)2 antivenom. Toxicon 59, 249-256. | ||
|
آمار تعداد مشاهده مقاله: 2,486 تعداد دریافت فایل اصل مقاله: 995 |
||