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شناسایی و کمّیت‌سنجی برخی ترکیبهای فلاونوئیدی در تعدادی از بیوتیپ‌های مرکبات | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 32، شماره 4 - شماره پیاپی 78، آبان 1395، صفحه 620-633 اصل مقاله (406.5 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.107134 | ||
| نویسندگان | ||
| مائده آهنکوب‌رو* 1؛ جواد فتاحی مقدم2؛ رضا فتوحی قزوینی3 | ||
| 1فارغ‌التحصیل کارشناسی ارشد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 2استادیار، پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری، مؤسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، رامسر، ایران | ||
| 3استاد، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| چکیده | ||
| بیوتیپ‌های طبیعی واقع در کلکسیون ژرم‌پلاسم پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری کشور از ذخایر ژنتیکی باارزش در کشور است که شاید مناسب تازه‌خوری نباشند ولی قابلیت استفاده در صنایع فرآوری را دارند. به این منظور از میوه‌های 16 بیوتیپ با کد‌های 6، 8، 15، 21، 24، 25،26، 29، 30، 41، 43، 45، 48، 51، 52 و 53 از این کلکسیون در دو مرحله، 60 روز پس از 50% شکوفایی گل‌ها (بعد از ریزش خردادماه) و 120 روز بعد از شکوفایی گل‌ها (آغاز بلوغ فیزیولوژی) نمونه‌برداری شد. علاوه‌بر اندازه‌گیری میزان فنل کل و ظرفیت آنتی‌اکسیدانی، مقداری ترکیبهای فلاونوئیدی شامل هسپریدین، نارینجین، کوئرستین و کاتچین نیز اندازه‌گیری شد. نتایج نشان داد که بیشترین مقدار کاتچین و کوئرستین 60 روز پس از 50% شکوفایی گل‌ها و در بیوتیپ 29 مشاهده شد. میزان هسپریدین و نارینجین در بین بیوتیپ‌ها روند متفاوتی داشت و بالاترین مقدار به‌ترتیب در بیوتیپ‌های 43 و 41 در 120 روز بعد از گلدهی مشاهده شد. به‌طور کلی ظرفیت آنتی‌اکسیدانی طی رشد روند کاهشی داشت ولی بیوتیپ‌های 6، 29، 43 و 51 بیشترین ظرفیت آنتی‌اکسیدانی را 60 روز پس از گلدهی داشتند. بالاترین میزان فنل کل در مرحله بلوغ میوه‌ها مشاهده شد. بیوتیپ با شماره 48 بالاترین میزان فنل کل را نسبت به سایر بیوتیپ‌ها نشان داد. به‌طور کلی بیوتیپ‌های 29، 41 و 43 دارای قابلیت بالایی از ترکیبهای فلاونوئیدی هستند که می‌توانند در برنامه‌های اصلاح ژنتیکی رقم و پایه، صنایع غذایی و دارویی مورد استفاده قرار گیرند. | ||
| کلیدواژه‌ها | ||
| ظرفیت آنتی‌اکسیدانی؛ بیوتیپ؛ فلاونوئید‌ها؛ فنل | ||
| عنوان مقاله [English] | ||
| Identifying and assaying the quantity of flavonoid compounds in some citrus biotypes | ||
| نویسندگان [English] | ||
| M. Ahankoub Ro1؛ J. Fattahi Moghadam2؛ R. Foutohi Ghazvini3 | ||
| 1M.Sc. Graduate student, Department of Horticulture Science, Faculty of Agriculture, Guilan University, Rasht, Iran | ||
| 2Horticultural Science Research Institute, Citrus and Subtropical Fruits Research Center, Agricultural Research Education and Extension Organization (AREEO), Ramsar, Iran. | ||
| 3Department of Horticulture Science, Faculty of Agriculture, Guilan University, Rasht, Iran | ||
| چکیده [English] | ||
| The natural biotypes in the germplasm collection of the Citrus and Subtropical Fruits Research Center are a valuable genetic resource in the country that may not be suitable for fresh eating but they can be used in processing industry. Therefore, the fruits of 16 biotypes, coded as 6, 8, 15, 21, 24, 25, 26, 29, 30, 41, 43, 45, 48, 51, 52 and 53, were harvested at two stages, 60 days after 50% of flowering (after June drop) and 120 days after 50% of flowering (beginning of physiological maturity). Some variables were measured such as total phenol and antioxidant capacity, and flavonoid compounds including hesperidin, naringin, quercetin and catechin content. Results showed that the maximum amount of catechin and quersetin was observed at 60 days after 50% of flowering in the biotype 29. The. amount of Hesperidin and Naringin was significantly different between biotypes. The highest amount of hesperidin and naringin was observed in biotypes 43 and 41 at 120 days after flowering, respectively. Generally, antioxidant capacity was reduced during of growth whereas biotypes 6, 29, 43, and 51 had the highest antioxidant capacity at 60 days after flowering. The highest total phenol content was observed at maturity stage of fruits. Biotype 48 showed higher amount of total phenol as compared with other biotypes. Totally, biotypes 29, 41, and 43 have high potential of flavonoid compounds to be used in the genetic improvement programs of cultivar and rootstock, food processing and pharmaceutical industry. | ||
| کلیدواژه‌ها [English] | ||
| antioxidant capacity, Biotype, Flavonoids, phenol | ||
| مراجع | ||
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