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In vitro study of drug-protein interaction using electronic absorption, fluorescence, and circular dichroism spectroscopy | ||
| Archives of Razi Institute | ||
| مقاله 6، دوره 71، شماره 3، آذر 2016، صفحه 183-194 اصل مقاله (449.33 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22034/ari.2016.106972 | ||
| نویسندگان | ||
| F. Attar* 1؛ S. Khavari-Nejad2 | ||
| 1Department of Biology, Faculty of Food Industry & Agriculture, Standard Research Institute, Karaj, Iran | ||
| 2Department of Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Karaj, Iran | ||
| چکیده | ||
| In the near future, design of a new generation of drugs targeting proteins will be required. Considering the complex bond between the drug and protein, the structure and stability of the target protein should be considered. So far, a series of in vitro investigations have been conducted with the aim of predicting drug-biological medium interactions. In these studies, use of spectroscopic methods, such as electronic absorption, fluorescence, and circular dichroism spectroscopy, which are briefly discussed in the present study, has been highlighted. The binding affinity of drug(s) to protein(s) and their binding mechanism(s) can be clearly determined by these methods, which reveal reactions in biological systems at low concentrations under physiological conditions. Ultraviolet-visible spectroscopy can be used as an accessible tool to measure slight changes in protein structure. Moreover, fluorescence spectroscopy provides tertiary structural information. On the other hand, circular dichroism spectroscopy in far-ultraviolet regions (180–260 nm) yields suitable information about different secondary structures of proteins. Conformational changes of proteins due to alterations such as physicochemical conditions, in vitro chemical modifications, and drug binding could impact ultraviolet-visible absorption, circular dichroism, and fluorescence spectra. Therefore, the study of changed spectra could reveal the structure-activity relationship of drug compounds and target proteins. In the present study, a short description of the mentioned methods, along with some related equations which are usually used to analyze and discuss the preliminary data, is presented. Overall, the obtained results could facilitate the development of biological and pharmaceutical potentials of drugs in the future. | ||
| کلیدواژه‌ها | ||
| In vitro study؛ Drug-protein interaction؛ Protein structure؛ Electronic | ||
| عنوان مقاله [English] | ||
| مطالعه آزمایشگاهی میانکنش دارو- پروتئین با استفاده از طیف سنجی جذب الکترونیکی، فلورسانس، و دورنگ نمایی دورانی | ||
| نویسندگان [English] | ||
| فرنوش عطار1؛ سارا خاوری نژاد2 | ||
| 1گروه مولکولار بیولوژی، دانشکده علوم زیستی، دانشگاه خوارزمی، کرج، ایران | ||
| 2بخش زیست شناسی، دانشکده صنایع غذایی و کشاورزی، موسسه تحقیقات استاندارد، کرج، ایران | ||
| چکیده [English] | ||
| علم پزشکی در آینده‌ای نه چندان دور نیاز به طراحی نسل جدیدی از داروهایی که پروتیئن‌ها را مورد هدف قرار می‌دهند، دارد. در کمپلکس شکل گرفته بین دارو و پروتئین، ساختار و پایداری پروتئین هدف باید در نظر گرفته شود. امروزه، بسیاری از مطالعات آزمایشگاهی برای پیش بینی میانکنش‌های بین دارو و محیط های بیولوژیکی انجام شده است. یکی از این مطالعات موثر، استفاده از روش‌های طیف سنجی مانند: جذب الکترونیکی، فلوئورسانس، و دورنگ نمایی دورانی است که به طور خلاصه در این مقاله مروری آورده شده است. با استفاده از روش‌های مذکور که امکان بررسی واکنش‌های سیستم‌های بیولوژیکی را در غلظت کم و در شرایط فیزیولوژیکی فراهم می آورند، تمایل اتصال دارو(ها) به پروتئین(ها) و مکانیسم(های) اتصالی آنها به وضوح مشخص می‌گردد. طیف سنجی مرئی-ماوراء بنفش میتواند به عنوان یک ابزار مهم در اندازه‌گیری تغییرات ظریف در ساختار پروتئین‌ها استفاده شود. طیف سنجی دورنگ نمایی دورانی نیز در منطقه دور ماورای بنفش (180-260 نانومتر) اطلاعات مناسبی را مورد اشکال مختلف ساختار دوم پروتئین و نهایتاً، طیف سنجی فلوئورسانس اطلاعات ساختار سوم پروتئین فراهم می‌کند. از آنجاییکه که تغییرات کنفورماسیون پروتئین‌ها به دلیل هرگونه تغییر از جمله شرایط فیزیکوشیمیایی، مدیفیکاسیون‌ها، و اتصالات دارو، میتواند بر طیف‌های مرئی-ماوراء بنفش، دورنگ نمایی دورانی، و فلوئورسانس تاثیر بگذارد، مطالعه طیف‌های تغییر یافته میتواند رابطه ساختار-فعالیت مواد دارویی و پروتئین‌های هدف را شرح دهد. در بررسی اجمالی حال حاضر، شرح کوتاهی از روش‌های ذکر شده همراه با برخی از معادلات مربوط به آنها که معمولاً در تجزیه و تحلیل داده‌ها بکار می‌روند، آورده شده است. در مجموع، نتایج این مقاله میتواند پایه و اساسی برای توسعه داروهای بالقوه بیولوژیکی در آینده باشد. | ||
| کلیدواژه‌ها [English] | ||
| مطالعه آزمایشگاهی, میانکنش دارو- پروتئین, ساختار پروتئین, طیف سنجی جذب الکترونی, طیف سنجی فلوئورسانس, طیف سنجی دورنگ نمایی دورانی | ||
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آمار تعداد مشاهده مقاله: 1,759 تعداد دریافت فایل اصل مقاله: 743 |
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